Bacteriologic Profile and Antibiogram of Blood Culture Isolates in a Pediatric Care Unit

Abstract

Background / Aims:Septicemia is one of the important causes of mortality and morbidity in neonates and children. Blood culture is the gold standard for the diagnosis. Emergence of multidrug resistant bacterial strains is a major problem in the management of sepsis. Present study was undertaken to identify the common bacterial pathogens associated with pediatric sepsis and to determine their antibiotic susceptibility pattern.Materials and Methods:Blood cultures from 185 suspected cases of sepsis were examined. The growths from the subcultures were identified by conventional biochemical tests. Antibiotic susceptibility testing was performed by modified Kirby-Bauer disk diffusion method and drug resistant strains in primary screening were further processed for extended spectrum beta lactamases (ESBL) and methicillin resistant Staphylococcus aureus (MRSA) status by combination disk method (ESBL) and oxacillin disk diffusion method (MRSA).Results:Out of the 185 cultures obtained from suspected cases, 81 (44%) were culture positive. Fifty-two (35%) of the culture isolates were Gram negative bacilli. Twenty-eight (64%) of the isolates were Gram positive cocci. One case was of mixed infection. The prevalence of MRSA in 41 strains of S. aureus was found to be 29% (12 strains). The overall prevalence of ESBL producers among 28 Gram negative bacterial isolates was found to be 32% (9 strains).Conclusion:This study stresses the need for the continuous screening and surveillance for antibiotic resistance in pediatric care unit.

A Study on the Bacteriological Profile and Antibiogram of Bacteremia in Children Below 10 Years in a Tertiary Care Hospital in Bangalore, India

“…Similar positivity rates were reported by other studies also [13,15]. Higher positivity rates of (43.78%) have been observed by Prabhu K et al, [9].…”

“…Gram positive organisms mainly Staphylococcus aureus have been shown as the most frequently isolated bacteria causing bacteremia by many studies [9,18]. The frequency of infection by various organisms varies from one institution to another institution and even year to year in the same institution [13].…”

“…One milliliter (neonates) and 5 ml (children) blood was collected and inoculated into 10 and 50 ml, respectively, of brain heart infusion broth (1:10 dilution). The culture bottles were incubated at 37ºC aerobically and periodic subcultures were done onto Mac Conkey's agar, blood agar and chocolate agar after overnight incubation on day 3, day 4 and finally on day 7 [9].The growth obtained was identified by conventional biochemical tests and the antibiotic sensitivity testing was performed on Mueller-Hinton agar plates by KirbyBauer disc diffusion method. Zone diameter was measured and interpreted as per the Clinical and Laboratory Standards Institute (CLSI) guidelines [10].…”

Bacteriological Profile and Antimicrobial Resistance of Blood Culture Isolates from a 350 bedded Hospital Lucknow, India

“…Entrobacter spp show lower resistant with Imipenem/Meropenem (8%), salmonella with Gentamycin (2%) and Pseudomanas in Clarithromycin 4%. Among Gram negative bacteria Amoxicillin noted as high resistant drug and Imepenem/ meropenem in some bacteria colistin was seen as a most sensitive drug similar to study of Kavitha Prabhu et al, (2010).…”

Blood Culture of Neonates in Paediatric Department and their Antimicrobial Susceptibility Pattern in and around Nims University, Jaipur, India

“…The incidence of sepsis is increasing all over the world leading to high morbidity and mortality rates (Kavitha et al, 2010). Detection of Bacteremia by blood culture is critical in managing patients with infection, and directs the appropriate selection of antimicrobials.…”