Bacteriological Evaluation of Some Fresh and Frozen fish

Ibrahim, Hemmat; Amin, Reham; Shawkey, Nahela; Mohammed, Hend Elham

doi:10.21608/bvmj.2016.31213

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…The occurrence of Pseudomonas aeruginosa in the examined samples may be attributed to the fact that this specie commonly found in human, animals, plants and are a zoonotic important. Lower results were obtained by Benie et al (2016), Ibrahim et al (2016) and Salem et al (2018).…”

Section: Discussionmentioning

confidence: 61%

Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt

Abd-El-Maogoud

Edris

Mahmoud

et al. 2021

SVU-International Journal of Veterinary Sciences

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The aims of this study were isolation and characterization of Pseudomonas spp. from fish meat collected from Sohag governorate, Egypt. A total 120 fish samples including frozen mackerel, frozen saurus, chilled Mugil cephalus and chilled Tilapia nilotica (30 of each) were collected from different shops and supermarkets in Sohag governorate. Pseudomonas spp. were isolated from 65% of the examined samples. The obtained data revealed that the highest count of Pseudomonas was in chilled Tilapia nilotica. The prevalence of Pseudomonas aeruginosa in frozen mackerel, frozen saurus, chilled Tilapia nilotica and chilled Mugil cephalus was 33.3%, 30%, 23.3% and 26.6% respectively. Furthermore, psychrotrophic count was performed and the results demonstrated that it was the highest in frozen mackerel followed by Tilapia nilotica and the Mugil cephalus showed the lowest count. Furthermore, the occurrence of oprL, phzM and toxA virulence genes was studied in some selected isolates by PCR. The findings showed that all the selected isolates possessed the virulence genes. This work showed contamination of fish samples with Pseudomonas spp., indicating the importance of applying hygienic measures during handling and storage of fish.

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Section: Discussionmentioning

confidence: 61%

Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt

Abd-El-Maogoud

Edris

Mahmoud

et al. 2021

SVU-International Journal of Veterinary Sciences

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“…In addition, the differences in the count among examined products were considered significant at (p<0.05). Aerobic plate count on fish is generally related to food safety risks and can sometimes be used to indicate quality, shelf life, and contamination after heat processing [28]. The findings of some authors [29,30] are very similar.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Bacteriological Quality of Some Fish Products

Elbarbary,

Abd El-aziz,

Maky

et al. 2024

Egyptian Journal of Veterinary Sciences

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F ISH products are considered a nutritionally significant component of the human diet because they contain macronutrients (proteins, fats, and ash) and micronutrients (vitamins and minerals). Accordingly, 200 different commercial fish product samples (25 each of canned solid tuna, canned chunks tuna, canned crumbled tuna, canned anchovy, canned mackerel, canned sardine, herring, and tilapia fillets) of different brands were collected from various local and high supermarkets in Aswan City during 2020-2021. The samples were subjected to microbial quality assessment, and PCR was carried out to detect some virulence genes of fish-borne pathogens to evaluate the control measures for food safety. The results presented that the highest bacterial loads (cfu/g) in the examined fish products were proven in fillets tilapia (1.3×104±0.32) and herring (1.0×104±1.2) while anchovy, mackerel, and crumbled tuna were the highest anaerobic count with means of 7.3×103±1.2, 7×103±1.6 and 6.7×103±1.4, respectively. The prevalence of P. aeruginosa occurs with an incidence of 29%. The lasB gene (50%) has been the most detected virulence gene, followed by exoS (40%). V. parahaemolyticus was present in 7.5%, and the regulator toxin genes (toxR and L.tdh) were found in 33.3% and 22.2%. Additionally, 20% of the samples had A. hydrophila, and the aerA and act genes were found in 60% of the isolates. In addition, C. perfringens was detected in 33% of examined products, and the virulence genes cpa and etx were present and discovered to be a proportion of 20% and 50%, respectively. The current study concluded that the examined fish products possess one or more food poisoning virulence genes indicating unsanitary handling, transport, processing, storage practices, and inappropriate environmental conditions.

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“…According to Henin 63 & Ibrahim et al . 64 , the incidence of Aeromonas spp. in imported frozen fish, fresh catfish and freshwater fish was reported as 15.2%, 11.6% and 9.7%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Microbiological quality assessment of Clarias gariepinus, Bagrus bajad, and Pangasianodon hypophthalmus fillets

El-Gendy,

Amer,

Ibrahim

et al. 2024

Sci Rep

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In this study, 80 catfish fillets were randomly collected from Egyptian local markets and retailers. The samples included 20 African catfish (Clarias gariepinus), 20 bayad (Bagrus bajad), and 40 pangasius catfish (Pangasianodon hypophthalmus) fillets. Pangasianodon hypophthalmus fillet samples were divided into 20 white basa and 20 red basa fillets. We conducted a microbiological analysis of catfish fillet samples, evaluating mesophilic aerobic bacteria, psychrophilic aerobic bacteria, H2S-producing bacteria, Staphylococcus spp., Enterobacteriaceae, Coliforms, and fecal Coliform counts. Additionally, we identified the existence of Salmonella spp., Vibrio spp., Yersinia spp., Escherichia spp., Aeromonas spp., and Pseudomonas spp. in the catfish fillet samples. In our study, the psychrophilic bacterial counts in Bagrus bajad (5.21 log CFU/g) were found to be higher compared to the counts in Clarias gariepinus (4.31 log CFU/g) and Pangasianodon hypophthalmus (3.89–4.7 log CFU/g). The fecal Coliform in Clarias gariepinus fillets was significantly higher than in other catfish fillets. We isolated Escherichia coli, Escherichia fergusonii, Aeromonas hydrophila, and Pseudomonas luteola from the catfish fillets, while no Salmonella spp., Vibrio spp., or Yersinia spp. were detected. These isolates were identified using 16S rRNA sequencing and phylogenetic analysis. Furthermore, ten Escherichia spp. were serologically identified, revealing that O26 and O78 were the most commonly occurring serotypes. This study highlights the microbiological analysis conducted on catfish fillets and concludes that the fillet samples from these catfish were of superior quality and deemed acceptable for human consumption.

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Bacteriological Evaluation of Some Fresh and Frozen fish

Cited by 6 publications

References 7 publications

Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt

Occurrence and characterization of Pseudomonas species isolated from Fish Marketed in Sohag Governorate, Egypt

Assessment of the Bacteriological Quality of Some Fish Products

Microbiological quality assessment of Clarias gariepinus, Bagrus bajad, and Pangasianodon hypophthalmus fillets

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