Total of hundred random samples of frozen chicken products represented by breast, thigh, nuggets and burger (25 of each) were collected from various supermarkets located in Menoufia government. To study the incidence of psychotropic bacteria with special reference to Pseudomonas species. The study revealed that mean values of Psychotropic count were 8.17×10 3 ± 1.42×10 3 , 1.95×10 4 ± 2.06×10 4 , 3.63×10 4 ± 0.89×10 4 and 7.58×10 4 ± 1.16×10 4 respectively while the mean value of Pseudomonas counts were 3.51×10 3 ± 0.76×10 3 , 6.29×10 3 ± 1.12×10 3 ,8.44×10 3 ± 1.85×10 3 , 1.71×10 4 ± 0.36×10 4 respectively for examined frozen breast, thigh, nuggets and burger. It was obvious that 166 isolates were identified as P.

A B S T R A C TThis study was conducted to evaluate the efficacy of three decontaminators in reducing the level of contamination in chicken carcasses which had been artificially contaminated. A grand total of forty random samples of raw chicken carcasses were purchased directly from local markets. Chicken samples were then dipped in cultures of the tested microorganisms (Salmonella spp., Staphylococcus aureus, E.coli O157:H7 and L. monoctogens) (10 samples for each microorganism). Then dipped into containers containing distilled water (control) , chlorine 30 ppm, 50 ppm and 70 ppm, trisodium phosphate 3%, 5% an 8% and lactic acid 0.75%, 1.25% and 2% , where the reduction percentage of Salmonella were 26.02 %, 26.57 %, 29.16 %, 34.88% , 36.78 %, 38.01 %, 35.42 %, 40.87 % and 54.50 %, respectively, compared to control. while the reduction of Listeria monocytogenes was 16.64 %, 20.60% , 30.43% , 21.74 %, 21.74 %, 25.14 %, 22.87 %, 30.24 % and 39.89 % respectively . Moreover , the reduction percentages of S. aureus was 14.29 % , 15.70 %, 19.58% , 8.11 %, 10.58 %, 27.69 %, 17.28 %, 21.34 % and 27.87 % , respectively . Finally, the reduction percentage of E.coli O157:H7 was 9.56 %, 16.61 %, 20.47 %, 17.45%, 21.48 %, 27.85 %, 18.12%, 19.97% and 29.70 %, respectively.

A total of 90 random samples of Frozen American, Brazilian and Indian meat samples (30 of each) were collected from different retail shops and supermarkets in EL-Menofeya Governorate at different production dates. The collected samples were examined bacteriologically and by using PCR tecnique for detection of Pseudomonas species, especially

Staphylococcus aureus (Staph. aureus)is Gram-positive bacterium commonly associated with food poisoning diseases. This pathogen is responsible for food-borne illnesses outbreaks associated with the consumption of food especially meat products. The aims of this study were to evaluate the ability of Staph. aureus recovered from food contact surfaces in meat processing environment of Egypt for biofilm formation and to test their susceptibility to the commercially used sanitizers including Quaternary ammonium compounds (QACs, 200ppm), Sodium hypochlorite (200ppm) and Iodine (2.5%). The ability of isolates for biofilm formation and their sensitivity to sanitizers were investigated in 96-well flat bottom microtiter plates. Twelve strains were obtained from 120 food contact surfaces (FCS) using standard microbiological techniques. All isolated strains 100% (12/12) manifested high ability to form biofilm, which was classified as a strong type. Further, the application of QACs, Sodium hypochlorite and iodine generated a reduction of (76.77%), (71.38%) and (15.84%) in the biofilm formation, respectively. Conclusively, all sanitizers were not 100% efficient in removing the biofilm formed by isolated Staph. aureus on polypropylene. Therefore, vigilant food safety practices need to be implemented in the meat processing environment, at meat processing plant and food services establishments, especially for FCS to prevent foodborne infections and intoxications due to S. aureus contamination from biofilms. BiofilmCross-contamination Food processing environment Sanitizers.

Sixty random samples of raw thigh & breast, half cooked chicken shawerma & shiesh tawook, fully cooked chicken shawerma and fully cooked chicken shiesh tawook (10 of each) were collected from different markets in Gharbia governorates and analyzed for biogenic amines by HPLC. The obtained results indicated that the mean values of histamine, cadaverine and putrescine (mg/100g) were 10.