Prevalence and molecular characterization of Pseudomonas species in frozen imported meat

Ibrahim, Hemmat; Hassan, Mohamed; El-Roos, Nahla A. bou; Elsalam, Mohga Abd

doi:10.21608/bvmj.2016.31301

Cited by 5 publications

(5 citation statements)

References 3 publications

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“…in chicken: P. libanensis , P. extremorientalis , P. antarctica , P. veronii , P. synxantha , P. marginalis , P. cedrina , P. koreensis , P. brenneri , and P. trivialis . The presence of P. orientalis has also been found in meat by other authors [ 50 ]. We also isolated other Pseudomonas spp., including P. rhodesiae , P. azotoformans , and P. kilorensis .…”

Section: Discussionsupporting

confidence: 61%

Microbiological Quality and Safety of Fresh Turkey Meat at Retail Level, Including the Presence of ESBL-Producing Enterobacteriaceae and Methicillin-Resistant S. aureus

Martínez‐Laorden

Arraiz‐Fernandez

González‐Fandos

2023

Foods

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The aim of this work was to study the microbiological safety and quality of marketed fresh turkey meat, with special emphasis on methicillin-resistant S. aureus, ESBL-producing E. coli, and K. pneumoniae. A total of 51 fresh turkey meat samples were collected at retail level in Spain. Mesophile, Pseudomonas spp., enterococci, Enterobacteriaceae, and staphylococci counts were 5.10 ± 1.36, 3.17 ± 0.87, 2.03 ± 0.58, 3.18 ± 1.00, and 2.52 ± 0.96 log CFU/g, respectively. Neither Campylobacter spp. nor Clostridium perfringens was detected in any sample. ESBL-producing K. pneumoniae and E. coli were detected in 22 (43.14%), and three (5.88%) samples, respectively, all of which were multi-resistant. Resistance to antimicrobials of category A (monobactams, and glycilcyclines) and category B (cephalosporins of third or fourth generation, polymixins, and quinolones), according to the European Medicine Agency classification, was found among the Enterobacteriaceae isolates. S. aureus and methicillin-resistant S. aureus were detected in nine (17.65%) and four samples (7.84%), respectively. Resistance to antimicrobials of category A (mupirocin, linezolid, rifampicin, and vancomycin) and category B (cephalosporins of third- or fourth generation) was found among S. aureus, coagulase-negative staphylococci, and M. caseolyticus isolates.

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Section: Discussionsupporting

confidence: 61%

Microbiological Quality and Safety of Fresh Turkey Meat at Retail Level, Including the Presence of ESBL-Producing Enterobacteriaceae and Methicillin-Resistant S. aureus

Martínez‐Laorden

Arraiz‐Fernandez

González‐Fandos

2023

Foods

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“…( 2016 ) reported that 97.90% of beef samples were positive for P. aeruginosa strains. Other surveys were conducted on chicken meat (Mahato et al., 2020 ), camel meat (Osman et al., 2019 ), cattle meat (Odoi et al., 2021 ), frozen meat (Ibrahim et al., 2016 ) and meat products (Sofy et al., 2017 ) samples showed that the P. aeruginosa prevalence had a range between 3.00 to 80.00%. High adaptation of P. aeruginosa strain in different temperatures (4–42°C) and water activities (72–97%) (Gu et al., 2016 ) may be another reason for its high prevalence among the examined samples.…”

Section: Discussionmentioning

confidence: 99%

Assessment of virulence factors and antimicrobial resistance among the Pseudomonas aeruginosa strains isolated from animal meat and carcass samples

Poursina

Ahmadi

Fazeli

et al. 2022

Veterinary Medicine & Sci

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Background Pseudomonas aeruginosa bacteria are emerging causes of food spoilage and foodborne diseases. Raw meat of animal species may consider a reservoir of P. aeruginosa strains. Objectives The present survey was done to assess the prevalence, antibiotic resistance properties and distribution of virulence factors among the P. aeruginosa strains isolated from raw meat and carcass surface swab samples of animal species. Methods Five hundred and fifty raw meat and carcass surface swab samples were collected from cattle and sheep species referred to as slaughterhouses. P. aeruginosa bacteria were identified using culture and biochemical tests. The pattern of antibiotic resistance was determined by disk diffusion. The distribution of virulence and antibiotic resistance genes was determined using polymerase chain reaction. Results Forty‐seven of 550 (8.54%) examined samples were contaminated with P. aeruginosa . The prevalence of P. aeruginosa in raw meat and carcass surface swab samples were 6.57 and 12%, respectively. P. aeruginosa isolates showed the maximum resistance rate toward penicillin (87.23%), ampicillin (85.10%), tetracycline (85.10%), gentamicin (65.95%) and trimethoprim (57.44%). The most commonly detected antibiotic resistance genes were BlaCTX‐M (53.19%), blaDHA (42.55%) and blaTEM (27.65%). The most commonly detected virulence factors was ExoS (42.55%), algD (31.91%), lasA (31.91%), plcH (31.91%) and exoU (25.53%). Conclusions Meat and carcass surface swab samples may be sources of resistant and virulent P. aeruginosa , which pose a hygienic threat in their consumption. However, further investigations are required to identify additional epidemiological features of P. aeruginosa in meat and carcass surface samples.

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“…fluorescence in imported frozen meat was 64.44%, 46.67% and 73.33%, respectively. Abd Elaal (2017) and Ibrahim et al (2016) those reported that the incidence of Ps. putida in imported frozen meat was (8.34%) and (8.34%) respectively.…”

Section: Disucssionmentioning

confidence: 97%

“…The incidence of Pseudomonas species isolated from the examined samples was 70%, 80% in frozen cubic meat and minced meat, respectively. Ibrahim, et al, (2016) those explained that the incidence of Ps. fluorescence in imported frozen meat was 64.44%, 46.67% and 73.33%, respectively.…”

Section: Disucssionmentioning

confidence: 99%

Phenotypic and genotypic characterization of pseudomonas species isolated from frozen meat.

Khalafallah

El-Tawab

Nada

et al. 2020

Benha Veterinary Medical Journal

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One hundred samples of imported Brazilian frozen meat (50 frozen cubic meat and 50 minced meat) were collected from different supermarkets in El-Menoufia Governorate, Egypt to be examined bacteriologically for detection of Pseudomonas species. The incidence of Pseudomonas species were (35/50) 70% in frozen cubic meat. While, the incidence of such organism in the examined frozen minced meat samples was 80% (40/50). Psychrotrophic bacterial count in the examined frozen cubic meat ranged from 6 x 10 2 to 1.9 x 10 5 with mean value 2.24 x 10 4 cfu/g. In addition, Psychrotrophic bacterial count in frozen minced meat ranged from 7 x 10 2 to 9 x 10 5 with mean value 1.7 x 10 5 cfu/g. The incidence of identified Pseudomonas species (number and percentages) detected in the examined samples of frozen meat represented by Ps. aeruginosa, Ps. fluorescence, Ps. diminuta, Ps putida and Ps. fragi were 15(30%), 40(80%), 8(16%), 5(10%) and 4(8%), respectively. Regarding the minced meat samples the incidence of identified Ps. aeruginosa, Ps. fluorescence, Ps. diminuta, Ps. putida, Ps. fragi were 20(40%), 45(90%), 5(10%), 7(14%) and 8(16%), respectively. The Pseudomonas species were resistant to Oxacillin. They were sensitive to Gentamycin except Ps. fluorescence. PCR is rapid and reliable tool for identification of different bacterial species.

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Prevalence and molecular characterization of Pseudomonas species in frozen imported meat

Cited by 5 publications

References 3 publications

Microbiological Quality and Safety of Fresh Turkey Meat at Retail Level, Including the Presence of ESBL-Producing Enterobacteriaceae and Methicillin-Resistant S. aureus

Microbiological Quality and Safety of Fresh Turkey Meat at Retail Level, Including the Presence of ESBL-Producing Enterobacteriaceae and Methicillin-Resistant S. aureus

Assessment of virulence factors and antimicrobial resistance among the Pseudomonas aeruginosa strains isolated from animal meat and carcass samples

Phenotypic and genotypic characterization of pseudomonas species isolated from frozen meat.

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