Abstract:Urinary tract infections (UTIs) are counted among the most common infections in humans. In spite of the availability and use of the antimicrobial drugs, UTIs caused by bacteria have been showing increasing trends. Patients diagnosed clinically as UTI during the study period (January 2015 to December 2015) in a Shri Vasantrao Naik Government Medical College and Hospital, Yavatmal (Maharashtra) were included in the study. Urine sample of these patients were tested for Culture and antibiotic susceptibility testing. A total of 472 urine samples were included in the study of that 368 from female patients and 104 from male patients. Out of 472 urine samples urine culture was positive in 200(42.37%) whereas in 272 urine culture was negative. Most common etiological organisms of UTI isolated were Escherichia coli 64(32%) followed by Klebsiella species 61(30.5%), Staphylococcus aureus 26(13%), Coagulase negative staphylococci (CoNS) 11(5.5), Pseudomonas aeruginosa 11(5.5%) Proteus species 7(3.5%), Citrobacter species 4(2%), Enterococci species 4(2%), Acinetobacter species 2(1%), Streptococcus pyogenes 1(0.5%). Most effective antibiotics were imipenem, nitrofurantoin, amikacin and piperacillin-tazobactum. For prevention of UTIs, implementation of strict infection control guidelines, effective hand washing and judicious use of antimicrobials is mandatory to prevent the emergence of drug resistance among uropathogens.