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Ventilator associated pneumonia (VAP) is a common and serious healthcare associated infection (HAI). VAP is inflammation of lung parenchyma caused by infectious agents that develops in a patient who is on a ventilator occurring 48-72 hrs or more after tracheal intubation and mechanical ventilation (MV). The risk of VAP is highest early in the course of hospital stay, and is estimated to be 3%/day during the first 5 days of ventilation, 2%/day during days 5-10 of ventilation and 1%/day after this. Aim of this study was find out the Microbial profile and antibiogram of ventilator associated pneumonia at tertiary care hospital U.P. This study was conducted in the Department of Microbiology, Rama Medical College, Hospital & Research Centre, Kanpur India from January 2016 to December 2016. A total of 100 Endotracheal aspirated samples were collected in a sterile & labelled clean dry container from the clinically suspected cases of VAP patients. Out of 100 patients 31 patients shown growth of the bacteria & considered as Ventilator Associated Pneumonia patients. And 69 patients have not shown any kind of growth. Acinetobacter baumanni, Pseudomonas aeruginosa, Enterobacter & Klebsiella Pneumoniae were the most common pathogens from both early & late onset VAP. Proper monitoring and strict implementation of infection control practices is very essential to reduce VAP in hospital setting. Also the short term use of invasive devices and judicious use of antibiotics are important in preventing VAP caused by these MDR pathogens.