Bacteriology of the burn wound at the bai jerbai wadia Hospital for children, Mumbai, India-A 13-year study, Part I-Bacteriological profile

Srinivasan, Shankar; Vartak, Arvind; Patil, Aakanksha; Saldanha, Jovita

doi:10.4103/0970-0358.59284

Cited by 14 publications

(5 citation statements)

References 12 publications

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“…A study carried out by Navendu et al in 2006 showed that 35.75% of Pseudomonas aeruginosa among infected burns patients, which is almost similar to present study [8]. A study carried out by Heggers et al in USA in 1998 showed that 51% of the burns patients are having infection of Pseudomonas aeruginosa, which has higher rate of infection as compare to present study [9].…”

Section: Discussionsupporting

confidence: 75%

Incidence of Multidrug Resistant Pseudomonas Aeruginosa Isolated From Burn Patients Tertiary Care Hospital, Jamnagar, Gujarat, India.

Kamaria¹,

Aring²,

Sinha³

2016

IOSR

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Section: Discussionsupporting

confidence: 75%

Incidence of Multidrug Resistant Pseudomonas Aeruginosa Isolated From Burn Patients Tertiary Care Hospital, Jamnagar, Gujarat, India.

Kamaria¹,

Aring²,

Sinha³

2016

IOSR

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“…Some previous studies have reported S. aureus as the predominant isolate from wounds 33,34. On the other hand, Srinivasan et al35 and Forson et al36 detected K. pneumoniae and P. aeruginosa as the predominant organisms, respectively. This difference, in predominance, may have resulted from variation in the applied treatment practices.…”

Section: Discussionmentioning

confidence: 91%

<p>The increasing threat of silver-resistance in clinical isolates from wounds and burns</p>

Hosny

Rasmy

Aboul-Magd

et al. 2019

IDR

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Purpose The widespread use of silver-containing compounds has led to emergence of silver-resistant bacteria. Few studies are available on the detectability of plasmid-mediated silver-resistance in developing countries. Therefore, we aimed to detect silver-resistance in isolates from wounds and burns, and to genetically characterize plasmid-mediated silver-resistance genes ( sil genes). Methods One hundred and fifty clinical isolates were obtained from burns and wounds. They were identified using the suitable Analytical Profile Index and MicroScan identification systems. Their antimicrobial susceptibility was tested by the disk diffusion and broth microdilution methods. Their silver nitrate (AgNO 3 ) minimum inhibitory concentration (MIC) was determined using the broth macrodilution method. The presence of different sil genes on plasmids extracted from silver-resistant isolates and the replicon types of the extracted plasmids were investigated using polymerase chain reaction (PCR). The ability of these plasmids to impart silver-resistance was tested by transformation. Results All except two isolates were multidrug-resistant. Nineteen silver-resistant bacterial isolates (12.6%) were detected; with AgNO 3 MIC ≥512 µg/mL. They were identified as Klebsiella pneumoniae (n=7), Staphylococcus aureus (n=4), Escherichia coli (n=2), Enterobacter cloacae (n=2), Pseudomonas aeruginosa (n=2) and Acinetobacter baumannii (n=2). PCR revealed the presence of different sil genes on the extracted plasmids. Plasmid transformation resulted in the transfer of silver-resistance to the resulting transformants. The extracted plasmids had different replicon types. Conclusion Plasmid-mediated silver-resistance was detected for the first time, in clinical P. aeruginosa, A. baumannii and S. aureus isolates; in addition to its detection in K. pneumoniae, E. coli and Enterobacter cloacae . Therefore, strict monitoring on the use of silver compounds in medical settings is required; with implementation of an approved standardized method for silver-resistance detection.

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“…cephalothin, carbeniciilin, ceftazidime (100%), and cephalexin (98%) respectively [7]. According to the study of Saha et al, it is most sensitive to beta lactams -imipenem (98.72%), followed by aztreonam (33.44%) and ceftazidime (38.32%) [8].…”

Section: Resultsmentioning

confidence: 99%

Incidence of Multidrug Resistant Pseudomonas Aeruginosa Isolated from Burn Patients and Environment of Teaching Institution

Biswal

Arora

Kasana

et al. 2014

JCDR

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Context: Pseudomonas aeruginosa is an important pathogen which causes nosocomial infections in immunocompromised patients, especially in hospitalized burn patients. In recent times, it has emerged as a widespread Multi Drug Resistant (MDR) pathogen which requires antibiotic susceptibility testing on a regular as well as a periodic basis. Aim of the study:The present study was undertaken to determine the antibiogram of P. aeruginosa which was isolated from inpatients and environmental sources, and to type the strains, based on their antibiogram patterns. Settings and Design:A prospective study was undertaken with 525 samples (blood and wound swabs) which were taken from 60 patients who were admitted to Vardhman Mahavir Medical College and Safdarjang hospital with burn injuries and with 101 samples which were obtained from environmental sources viz. surgical instruments, dressings, suction devices, sinks, antiseptic solutions, etc. Materials and Methods:The strains were cultured and identified by standard microbiological techniques and Kirby-Bauer disc diffusion antibiotic susceptibility testing was done for each. Statistical analysis:Chi-squire tests were done and p-values of less than 0.05 were considered to be significant. Results:Fifty six strains and two strains, respectively, of P. aeruginosa were isolated from inpatients and environmental samples (one strain from sink and one strain from door wall, among the two) respectively. In total, 58 (81%) P. aeruginosa strains were found to be resistant to aminoglycosides, 41-70% were resistant to beta-lactams -piperacillin, ceftazidime, and aztreonam, 34.5% were resistant to piperacillin-tazobactam, 12.06% were resistant to ciprofloxacin and 13-19% were resistant to carbapenems. All strains were sensitive to colistin. P. aeruginosa was resistant to three of the four 'in-use' drugs i.e. piperacillin+tazobactam, imipenem, ceftazidime, and gentamicin, which was taken as MDR, which depicted MDR percentage as 36.2 (21/58). Conclusion:Strategies of optimal prescribing, including control of antibiotic usage, coupled with periodic studies on MDR P. aeruginosa infections in burn patients, appear to be leading priorities which help in improving therapeutic gains in such patients.

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Bacteriology of the burn wound at the bai jerbai wadia Hospital for children, Mumbai, India-A 13-year study, Part I-Bacteriological profile

Cited by 14 publications

References 12 publications

Incidence of Multidrug Resistant Pseudomonas Aeruginosa Isolated From Burn Patients Tertiary Care Hospital, Jamnagar, Gujarat, India.

Incidence of Multidrug Resistant Pseudomonas Aeruginosa Isolated From Burn Patients Tertiary Care Hospital, Jamnagar, Gujarat, India.

<p>The increasing threat of silver-resistance in clinical isolates from wounds and burns</p>

Incidence of Multidrug Resistant Pseudomonas Aeruginosa Isolated from Burn Patients and Environment of Teaching Institution

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