Bacteriophage Reactions and Speciation of Phytopathogenic Xanthomonads

Stolp, H.; Starr, Mortimer P.

doi:10.1111/j.1439-0434.1964.tb03451.x

Cited by 46 publications

(17 citation statements)

References 24 publications

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“…After incubation at 28-308C for 4-5 days, colonies tentatively identified as Xanthomonas spp. based on morphology are subcultured on Yeast Dextrose Calcium Carbonate Agar (YDCA) (Stolp & Starr, 1964). Identification is confirmed by classical bacteriological tests (morphology of the colonies on YDCA, hydrolysis of amylose in BS or BSCAA-agar, hydrolysis of esculin and casein, growth at 358C, acidity from glucose, arabinose and mannose) (Schaad, Jones, & Lacy, 2002) and inoculation on susceptible Brassica seedlings (Roberts & Koenraadt, 2003).…”

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Detection and identification of the crucifer pathogen, Xanthomonas campestris pv. campestris, by PCR amplification of the conserved Hrp/type III secretion system gene hrcC

et al. 2007

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The development of a rapid detection method for Xanthomonas campestris pv. campestris (Xcc) in crucifer seeds and plants is essential for high-throughput certification purposes. Here we describe a diagnostic protocol for the identification/ detection of Xcc by PCR amplification of fragments from the pathogenicity-associated gene hrcC. Under stringent conditions of amplification, a PCR product of 519 bp from hrcC was obtained from a collection of 46 isolates of Xcc, with the exception of two isolates from radish. No amplicons were obtained from 39 pure cultures of the phytopathogenic bacteria Xanthomonas campestris pv. cerealicola, X. campestris pv. juglandis, X. campestris pv. pelargonii, X. campestris pv. vitians, X. arboricola pv. pruni, X. axonopodis pv. phaseoli, X. axonopodis pv. vesicatoria, X. vesicatoria, Pseudomonas syringae pv. phaseolicola, P. syringae pv. syringae, P. syringae pv. tomato, P. fluorescens, P. marginalis, Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum. In addition, PCR reactions were negative for fifty unidentified environmental isolates M. Zaccardelli (&) Á F. Campanile Á A. Spasiano C.R.A-Istituto Sperimentale per le Colture Industriali, SS 18 n8 204,

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“…Xanthomonas spp. were routinely grown on YDCA (Stolp & Starr, 1964); Pseudomonas spp. were grown on King's Medium B Agar (King, Ward, & Raney, 1954); Erwinia spp., Pectobacterium spp.…”

mentioning

confidence: 99%

Detection and identification of the crucifer pathogen, Xanthomonas campestris pv. campestris, by PCR amplification of the conserved Hrp/type III secretion system gene hrcC

et al. 2007

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“…The single extant comparative study -at anything close to a comprehensive level-of the bacteriophage relationships in the genus Xanthomonas is that reported by Stolp and Starr (1964). It might be useful to summarize these findings here, with particular emphasis on their possible applications to solving the taxonomic enigmas in this bacterial genus.…”

Section: Bacteriophage Relationships In Xanthomonasmentioning

confidence: 96%

“…The notion that there are many sub generic taxa in Xanthomonas is supported by the somewhat ambiguous phytopathogenic specialization, by DNA-DNA segmental homology (Murata and Starr, 1973), by phage typing data (Dye, Starr, and Stolp, 1964;Stolp and Starr, 1964), by serology (Elrod and Braun, 1947a,b,c), by electrophoretic mobility of proteins (EI-Sharkawy and Huisingh, 1971a,b), and by ribosome immunology (Schaad, 1976(Schaad, , 1978. On the other hand, the notion that there are many sub generic taxa in Xanthomonas seems to be denied by both fragmentary and fairly extensive numerical and other phenetic taxonomy (Colwell and Liston, 1961;Colwell, Moffett, and Sutton, 1968;Dye, 1962;, by the probe into rRNA-DNA hybridization (Palleroni et aI., 1973), and by the earlier DNA-DNA homology data De Ley et aI., 1966;Friedman and De Ley, 1965).…”

Section: Characterization and Identificationmentioning

confidence: 98%

The Genus Xanthomonas

Starr¹

1981

The Prokaryotes

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“…YDC agar medium (Stolp & Starr, 1964): yeast extract 10.0 g; dextrose (glucose) 20.0 g; calcium carbonate (light powder) 20.0 g; agar 15.0 g; distilled water to 1.0 L YPG agar medium (Lelliott & Stead, 1987): yeast extract 5.0 g; bacteriological peptone 5.0 g; glucose 10.0 g; agar 20.0 g; distilled water to 1 L; (pH 6.5-7.0).…”

Section: Appendix I Mediamentioning

confidence: 99%