Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model

Iwano, Hidetomo; Inoue, Yusuke; Takasago, Takuji; Kobayashi, Hironori; Furusawa, Takaaki; Taniguchi, Kotomi; Fujiki, Jumpei; Yokota, Hiroshi; Usui, Masaru; Tanji, Yasunori; Hagiwara, Kaoru; Higuchi, Hidetoshi; Tsutsumi, Yutaka

doi:10.3390/biology7010008

Cited by 60 publications

(62 citation statements)

References 32 publications

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“…Other studies, which investigated the use of phages in animal experiments already showed promising results [22,46,47]. Capparelli et al [23] showed the activity of S. aureus bacteriophages against local and systemic S. aureus infections as well as intracellular and methicillin resistant strains of S. aureus (MRSA) in mice.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Bacteriophages Against Staphylococcus aureus Isolates from Bovine Mastitis

Titze¹,

Lehnherr²,

Lehnherr³

et al. 2020

Pharmaceuticals

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The lytic efficacy of bacteriophages against Staphylococcus aureus isolates from bovine milk was investigated in vitro, regarding possible applications in the therapy of udder inflammation caused by bacterial infections (mastitis). The host range of sequenced, lytic bacteriophages was determined against a collection of 92 Staphylococcus (S.) aureus isolates. The isolates originated from quarter foremilk samples of clinical and subclinical mastitis cases. A spot test and a subsequent plaque assay were used to determine the phage host range. According to their host range, propagation and storage properties, three phages, STA1.ST29, EB1.ST11, and EB1.ST27, were selected for preparing a bacteriophage mixture (1:1:1), which was examined for its lytic activity against S. aureus in pasteurized and raw milk. It was found that almost two thirds of the isolates could be lysed by at least one of the tested phages. The bacteriophage mixture was able to reduce the S. aureus germ density in pasteurized milk and its reduction ability was maintained in raw milk, with only a moderate decrease compared to the results in pasteurized milk. The significant reduction ability of the phage mixture in raw milk promotes further in vivo investigation.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Bacteriophages Against Staphylococcus aureus Isolates from Bovine Mastitis

Titze¹,

Lehnherr²,

Lehnherr³

et al. 2020

Pharmaceuticals

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“…It was postulated that the latter two methods were better in circulating the phages throughout the whole of the mammary glands. However, in all three phage administrations, S. aureus proliferation was reduced (Iwano et al, ). In a bovine mastitis study conducted by Gill et al (), a 16.7% cure rate of S. aureus mastitis was reported when using phage K (in contrast to 0% with phage‐free controls), but it is possible that a higher cure rate would have been achieved had multiple phages been used.…”

Section: Veterinary Applications Of Phages In Dairy Productionmentioning

confidence: 99%

The use of bacteriophages to control and detect pathogens in the dairy industry

O’Sullivan

Bolton

McAuliffe

et al. 2019

Int J of Dairy Tech

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Dairy products are susceptible to bacterial contamination during both primary production and the processing stages. Pasteurisation and high hydrostatic pressure are used to eliminate bacteria from milk; however, some heat‐tolerant pathogens and bacterial spores may survive these processes. Bacteriophages (phages) are viruses that infect and lyse specific bacteria. These or their encoded proteins may be exploited for pathogen biocontrol in milk products and for mastitis treatment during primary production. The host specificity of phages and their proteins may also be exploited for pathogen detection. Various examples of phage‐mediated pathogen biocontrol and detection in the dairy context are discussed in this review.

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“…Phage therapy has attracted a great deal of attention as a strategy against MRSA infections and to provide a new solution against the threat of MDR infections (19). This approach uses bacterial viruses (phages) that can specifically attack pathogenic bacteria and kill them (20). Phage therapy has the potential of being highly specific merely against a species or even a strain responsible for infection (8,21,22).…”

Section: Introductionmentioning

confidence: 99%

Characterizing a Lytic Bacteriophage Infecting Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Burn Patients

Hallajzadeh

Mojtahedi

Amirmozafari

et al. 2020

Arch Clin Infect Dis

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant threat to human health. It is a multidrug-resistant (MDR) pathogen capable of causing a variety of diseases. Also, MRSA is one of the most important nosocomial pathogens in burn infection. As a treatment strategy against MRSA infections, phage therapy has the potential of becoming an alternative remedy. Objectives: The present study aimed to isolate and characterize a lytic bacteriophage from hospital sewage to be effective against burn wound-infecting MRSA isolates. Methods: Staphylococcus aureus strains were isolated from hospitalized burn patients. The strains were confirmed as MRSA by the Kirby-Bauer disk diffusion method using penicillin, methicillin, and oxacillin, as well as the PCR assay for the mecA gene. The phage was isolated from the hospital sewage and tittered by the double layer agar (DLA) method. The spot test was used for host range determination. The latent period and burst size were estimated from a one-step growth curve. The phage morphology was observed by electron microscopy. The nature of the nucleic acid of the isolated bacteriophages was confirmed by Rnase A, Dnase I, and six restriction enzymes. Results: The titer, latent period, and burst size of the isolated phage were determined to be 1 × 10 9 PFU/mL, 20 min, and 190 PFU per infected cell, respectively. It displayed a broad host range for MRSA bacteria by the spot test (27 out of 30 isolates). Electron microscopy observation demonstrated that the phage belonged to the Myoviridea family. Digestion profiles of Rnase A, Dnase I, and six restriction enzymes in 1% agarose gel electrophoresis showed that the genome of the isolated phage was a double-stranded DNA with a size of <~23 kbp. Conclusions: The isolated phage (MH-1) was active against a wide range of MRSA strains recovered from burn patients. Its specificity and remarkable lytic effects on MRSA strains emphasized that it could be a suitable candidate for use in prophylaxis and treatment of these clinical infections.

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Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model

Cited by 60 publications

References 32 publications

Efficacy of Bacteriophages Against Staphylococcus aureus Isolates from Bovine Mastitis

Efficacy of Bacteriophages Against Staphylococcus aureus Isolates from Bovine Mastitis

The use of bacteriophages to control and detect pathogens in the dairy industry

Characterizing a Lytic Bacteriophage Infecting Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Burn Patients

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