2015
DOI: 10.1186/s12985-015-0395-0
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Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla

Abstract: BackgroundThe study of bacteriophages continues to generate key information about microbial interactions in the environment. Many phenotypic characteristics of bacteriophages cannot be examined by sequencing alone, further highlighting the necessity for isolation and examination of phages from environmental samples. While much of our current knowledge base has been generated by the study of marine phages, freshwater viruses are understudied in comparison. Our group has previously conducted metagenomics-based s… Show more

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Cited by 79 publications
(80 citation statements)
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“…Such population-genetic assessment of phage evolution, coupled with the ecological implications of genome heterogeneity, will inform how to define nodes in future iterations of the ecological network developed here. Even though we are hesitant to speculate on phage host ranges at low taxonomic levels in our dataset, the data does agree with previous reports of instances of broad phage host range [60,75]. Additionally, visualization of our dataset interactions using the heat map approach previously used in other host range studies, suggests a trend toward modular and nested tropism, but we do not have the strain-level resolution that powered those previous experimental studies.…”
Section: Discussionsupporting
confidence: 75%
“…Such population-genetic assessment of phage evolution, coupled with the ecological implications of genome heterogeneity, will inform how to define nodes in future iterations of the ecological network developed here. Even though we are hesitant to speculate on phage host ranges at low taxonomic levels in our dataset, the data does agree with previous reports of instances of broad phage host range [60,75]. Additionally, visualization of our dataset interactions using the heat map approach previously used in other host range studies, suggests a trend toward modular and nested tropism, but we do not have the strain-level resolution that powered those previous experimental studies.…”
Section: Discussionsupporting
confidence: 75%
“…Host prediction results on crAssphage also highlight how the \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$d_2^*$\end{document} method can be useful for hypothesis generation. It is generally recognized that viruses rarely infect hosts from multiple phyla, but a few cases have been reported (53). The \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$d_2^*$\end{document} method predicted two possible host phyla for crAssphage, Bacteroidetes and Fusobacteria, which could be tested directly if and when crAssphage viruses are isolated.…”
Section: Discussionmentioning
confidence: 99%
“…The previously defined Enterobacteriaceae authentic phage clusters very rarely include phages that are known to infect hosts from other bacterial taxonomic families; we have found only two exceptions. Stenotrophomonas (a member of the Xanthomonadaceae bacterial family) phage IME15 is remarkable in its inclusion within the Enterobacteriaceae T7-like phage cluster (lytic cluster 5 in Grose and Casjens, 2014), and recently several phages that belong to the EMCL-117-like cluster (lytic cluster 27) have been reported to be broad spectrum, infecting both E. coli in the Enterobacteriaceae family and Pseudomonas aeruginosa in the Pseudomonadaceae family (Malki et al , 2015). …”
Section: Introductionmentioning
confidence: 99%