Bacteriophages with potential to inactivate Salmonella Typhimurium: Use of single phage suspensions and phage cocktails

Pereira, Carla; Moreirinha, Catarina; Lewicka, Magdalena; Almeida, Paulo; Clemente, Carla; Cunha, Ângela; Delgadillo, Ivonne; Romalde, Jesús L.; Nunes, María Leonor; Almeida, Adelaide

doi:10.1016/j.virusres.2016.04.020

Cited by 99 publications

(79 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Compared to literature values, the latent period for LPSEYT is shorter than other myoviruses, such as Salmonella phage vB_SnwM_CGG4-2 (about 58 min), Vibrio harveyi phage VhKM4 (about 60 min), and Stenotrophomonas maltophilia phage ϕSMA5 (about 80 min) [39][40][41]. At the same time, its burst size was greater than VhKM4 (about 52 PFU/cell), phSE-1 (about 28 PFU/mL), and phSE-2 (about 53 PFU/mL) [28,40], which is likely to be a reflection of high lytic activity. The effects of pH and temperature on the stability of LPSEYT were evaluated in this study.…”

Section: Morphology and Biological Characteristics Of Lpseytmentioning

confidence: 91%

See 1 more Smart Citation

Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods

et al. 2020

View full text Add to dashboard Cite

Salmonella is a leading cause of foodborne diseases, and in recent years, many isolates have exhibited a high level of antibiotic resistance, which has led to huge pressures on public health. Phages are a promising strategy to control food-borne pathogens. In this study, one of our environmental phage isolates, LPSEYT, was to be able to restrict the growth of zoonotic Salmonella enterica in vitro over a range of multiplicity of infections. Phage LPSEYT exhibited wide-ranging pH and thermal stability and rapid reproductive activity with a short latent period and a large burst size. Phage LPSEYT demonstrated potential efficiency as a biological control agent against Salmonella in a variety of food matrices, including milk and lettuce. Morphological observation, comparative genomic, and phylogenetic analysis revealed that LPSEYT does not belong to any of the currently identified genera within the Myoviridae family, and we suggest that LPSEYT represents a new genus, the LPSEYTvirus. This study contributes a phage database, develops beneficial phage resources, and sheds light on the potential application value of phages LPSEYT on food safety.

show abstract

Section: Morphology and Biological Characteristics Of Lpseytmentioning

confidence: 91%

“…Spot test was carried out according to reported methodology [28]. Overnight cultures of bacteria were mixed with TSB medium supplemented with 0.7% agar and then poured onto a pre-set TSA bottom layer to form bacterial lawn.…”

Section: Phage Spot Testmentioning

confidence: 99%

Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Putative BIMs were isolated according to methods described by Pereira et al [53]. Briefly, 5 µL of a phage SI1 suspension (~10 9 PFU/mL) were spotted onto bacterial lawns consisting of 300 µL of stationary phase cultures of S. Agona mixed with 4 mL 0.7% TSA overlaid onto 1.5% TSA in Petri plates.…”

Section: Isolation Of Bims Of S Agona Fsl S5-517mentioning

confidence: 99%

Bacteriophage-Insensitive Mutants of Antimicrobial-Resistant Salmonella Enterica are Altered in their Tetracycline Resistance and Virulence in Caco-2 Intestinal Cells

Fong

Rheault

et al. 2020

IJMS

View full text Add to dashboard Cite

Bacteriophages have shown promise as therapeutic alternatives to antibiotics for the control of infectious bacteria, including the human pathogen Salmonella. However, the development of effective phage-based applications requires the elucidation of key interactions between phages and target hosts, particularly since host resistance to phage is inevitable. Little is known about the alteration of host phenotypes following the development of resistance to phage. The aim of this study is to evaluate the antibiotic susceptibility and virulence of a Salmonella isolate following the development of resistance to bacteriophage SI1. We observed enhanced susceptibility to tetracycline and decreased invasion capacity in a differentiated Caco-2 intestinal cell line. Whole genome sequence analysis revealed an array of mutations, most notably, truncations in vgrG1_2, a core gene involved in Type VI secretion and mutations in the lipopolysaccharide, thereby indicating the plausible attachment site of phage SI1. These findings shed light on understanding the underlying mechanism for phage immunity within the host. Importantly, we reveal an associated genetic cost to the bacterial host with developing resistance to phages. Taken together, these results will aid in advancing strategies to delay or eliminate the development of host resistance when designing informed phage-based antimicrobials.

show abstract

“…Phages have been used for decades to effectively treat bacterial infectious diseases, including wound and gastrointestinal infections (Sulakvelidze et al, 2001; Sulakvelidze and Morris, 2001; Sulakvelidze and Kutter, 2005; Sulakvelidze, 2011) as well as to reduce food borne illnesses, including those caused by V. parahaemolyticus or Salmonella (Tan et al, 2014; Letchumanan et al, 2016; Pereira et al, 2016a,b). Commercial phages are now available for treating bacterial diseases in humans, animals (including aquaculture) and agricultural crops (Housby and Mann, 2009; Ly-Chatain, 2014).…”

Section: Preventive Treatments: Present and Future Prospectsmentioning

confidence: 99%

New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

2017

View full text Add to dashboard Cite

Hatcheries constitute nowadays the only viable solution to support the husbandry of bivalve molluscs due to the depletion and/or overexploitation of their natural beds. Hatchery activities include the broodstock conditioning and spawning, rearing larvae and spat, and the production of microalgae to feed all stages of the production cycle. However, outbreaks of disease continue to be the main bottleneck for successful larval and spat production, most of them caused by different representatives of the genus Vibrio. Therefore, attention must be paid on preventive and management measures that allow the control of such undesirable bacterial populations. The present review provides an updated picture of the recently characterized Vibrio species associated with disease of bivalve molluscs during early stages of development, including the controversial taxonomic affiliation of some of them and relevant advances in the knowledge of their virulence determinants. The problematic use of antibiotics, as well as its eco-friendly alternatives are also critically discussed.

show abstract

Bacteriophages with potential to inactivate Salmonella Typhimurium: Use of single phage suspensions and phage cocktails

Cited by 99 publications

References 64 publications

Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods

Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods

Bacteriophage-Insensitive Mutants of Antimicrobial-Resistant Salmonella Enterica are Altered in their Tetracycline Resistance and Virulence in Caco-2 Intestinal Cells

New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

Contact Info

Product

Resources

About