Bacteriostatic activity of aquatic extract of black peel pomegranate and silver nanoparticles biosynthesized by using the extract

Khorrami, Sadegh; Kamali, F; Zarrabi, Ali

doi:10.1016/j.bcab.2020.101620

Cited by 33 publications

(19 citation statements)

References 32 publications

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“…One AU is defined as the reciprocal of the highest dilution resulting in a distinct growth inhibition on the indicator strain (Banerjee, Dora, & Chowdhury, 2013). Besides, the antibacterial activity of the bacteriocin taken from the 10‐hr and 20‐hr cultures (early log phase and early stationary phase, respectively) was compared by the well diffusion method (Khorrami, Kamali, & Zarrabi, 2020).

A U {ml}^{‐ 1} = r e c i p r o c a l o f h i g h e s t t w o f o l d d i l u t i o n \times 100

…”

Section: Methodsmentioning

confidence: 99%

Bacteriostatic activity and partial characterization of the bacteriocin produced by L. plantarum sp. isolated from traditional sourdough

Zangeneh

Khorrami

Khaleghi

2020

Food Science & Nutrition

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Although rapid advances occurred in food processing technologies, preservation of nourishments for a long-term period has always been one of the most challenging issues. Meanwhile, the demand for healthy, ready food is increasing in the modern world, putting the industry under pressure to meet these requirements and being profitable too. On the other hand, the bacterial resistance to antibiotics and dwindling ready foods' quality have raised many concerns. To solve these problems, many experts have recently focused on biological agents, such as plant extracts, animal-derived enzymes, organic acids, and probiotic bacterial strains (Del Nobile, Lucera, Costa, & Conte, 2012). Among these strategies, probiotics are in a great position due to their positive influences on the function of the human gastrointestinal tract as well as their economic features. That is why during the last decades, a large number of academic investigations have been being conducted in this area. The antimicrobial activity of probiotics is considered as an effective means to eliminate or inhibit pathogens (Dicks & Botes, 2010). Some probiotics through secreting antibacterial compounds, such

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A U {ml}^{‐ 1} = r e c i p r o c a l o f h i g h e s t t w o f o l d d i l u t i o n \times 100

…”

Section: Methodsmentioning

confidence: 99%

Bacteriostatic activity and partial characterization of the bacteriocin produced by L. plantarum sp. isolated from traditional sourdough

Zangeneh

Khorrami

Khaleghi

2020

Food Science & Nutrition

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“…The complete mechanism of Ag NPs and bacterial inhibition was still unknown. But, it can enter into the metabolic pathway of cancer cells and inhibit the nucleus of the cells completely ( Khorrami et al, 2020 ). Recent reports are clearly stated that the plant mediated Ag NPs synthesis was excellent method and inhibit the cancer cells at very lowest concentration.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesized silver nanoparticles using Caulerpa taxifolia against A549 lung cancer cell line through cytotoxicity effect/morphological damage

Zhang

Ramachandran

Mothana

et al. 2020

Saudi Journal of Biological Sciences

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The Caulerpa taxifolia is excellent marine green algae, which produced enormous bioactive compounds with more biological activities. Also, it is an excellent source for synthesis of Ag NPs with increased bioactivity against various infections. In our study, the marine algae marine algae Caulerpa taxifolia mediated Ag NPs was synthesized effectively. The synthesized Ag NPs was characterized well using UV-spectrometer and X-ray powder diffraction (XRD) and confirmed as synthesized particle was Ag NPs. The available structure of the Ag NPs was morphologically identified by scanning electron microscope (SEM), and exact minimum size, polydispersive spherical shape of the entire Ag NPs structure was confirmed by Transmission electron microscope (TEM). Further, the anti-cancer efficiency of biosynthesized Ag NPs against A549 lung cancer cells was found at 40 µg/mL concentration by cytotoxicity experiment. In addition, the phase contrast images of the result were supported the Ag NPs, which damaged the A549 morphologically clearly. Finally, florescence microscopic images were effectively proved the anti-cancerous effect against A549 lung cancer cells due to the condensed morphology of increased death cells. All the confirmed in-vitro results were clearly stated that the Caulerpa taxifolia mediated Ag NPs has superior anti-cancer agent against A549 lung cancer cells.

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“…The biologically synthesized nanoparticles were active against both gram-positive and gram-negative bacteria. Such promising results could be related to both silver nanoparticles and the extract components that cover them (Alyousef et al 2019 ; Khorrami et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…The antibacterial activity of the extract against the isolated MRSA was evaluated by the well-diffusion method (Khorrami et al 2020 ). First, the wells with 6 mm diameter were embedded on Muller-Hinton agar medium.…”

Section: Methodsmentioning

confidence: 99%

Down-regulation of biofilm-associated genes in mecA-positive methicillin-resistant S. aureus treated with M. communis extract and its antibacterial activity

Khaleghi

Khorrami

2021

AMB Expr

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Considering the prevalence of resistance to antibiotics, the discovery of effective agents against resistant pathogens is of extreme urgency. Herein, 26 mecA-positive methicillin-resistant S. aureus (MRSA) isolated from clinical samples were identified, and their resistance to 11 antibiotics was investigated. Next, the antibacterial and anti-biofilm activity of the ethanolic extract of M. communis on these strains was evaluated. Furthermore, the effect of this extract on the expression of biofilm-associated genes, icaA, icaD, bap, sarA, and agr, was studied. According to the results, all isolated strains were multidrug-resistant and showed resistance to oxacillin and tetracycline. Also, 96.15 and 88.46 % of them were resistant to gentamicin and erythromycin. However, the extract could effectively combat the strains. The minimum inhibitory concentration (MIC) against different strains ranged from 1.56 to 25 mg/ml and the minimum bactericidal concentration (MBC) was between 3.125 and 50 mg/ml. Even though most MRSA (67 %) strongly produced biofilm, the sub-MIC concentration of the extract destroyed the pre-formed biofilm and affected the bacterial cells inside the biofilm. It could also inhibit biofilm development by significantly decreasing the expression of icaA, icaD, sarA and bap genes involved in biofilm formation and development. In conclusion, the extract inhibits biofilm formation, ruins pre-formed biofilm, and kills cells living inside the biofilm. Furthermore, it down-regulates the expression of necessary genes and nips the biofilm formation in the bud.

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Bacteriostatic activity of aquatic extract of black peel pomegranate and silver nanoparticles biosynthesized by using the extract

Cited by 33 publications

References 32 publications

Bacteriostatic activity and partial characterization of the bacteriocin produced by L. plantarum sp. isolated from traditional sourdough

Bacteriostatic activity and partial characterization of the bacteriocin produced by L. plantarum sp. isolated from traditional sourdough

Biosynthesized silver nanoparticles using Caulerpa taxifolia against A549 lung cancer cell line through cytotoxicity effect/morphological damage

Down-regulation of biofilm-associated genes in mecA-positive methicillin-resistant S. aureus treated with M. communis extract and its antibacterial activity

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