Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

Granziero, Luisa; Nelboeck, Peter; Bedoucha, Marc; Lanzavecchia, Antonio; Reid, Hugh Harrington

doi:10.1016/s0022-1759(97)00018-5

Cited by 17 publications

(12 citation statements)

References 17 publications

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“…This will accelerate the screening and generation of recombinant viruses. Third, recombinant bacmids can be generated at high frequency (∼10 7 c.f.u./μg) virtually free of background (12), which yields high virus diversity more efficiently than traditional homologous recombination methods (34) or methods based on conventional restriction enzyme cloning (35). Fourth, because no restriction enzyme digestions are required for cloning, the yield of full-length clones is improved since the inserts will not be cut from internal restriction sites.…”

Section: Discussionmentioning

confidence: 99%

A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo

Laitinen

Airenne²,

Hytönen³

et al. 2005

Nucleic Acids Research

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We have constructed a novel tetra-promoter vector (pBVboostFG) system that enables screening of gene/cDNA libraries for functional genomic studies. The vector enables an all-in-one strategy for gene expression in mammalian, bacterial and insect cells and is also suitable for direct use in vivo. Virus preparation is based on an improved mini Tn7 transpositional system allowing easy and fast production of recombinant baculoviruses with high diversity and negligible background. Cloning of the desired DNA fragments or libraries is based on the recombination system of bacteriophage lambda. As an example of the utility of the vector, genes or cDNAs of 18 different proteins were cloned into pBVboostFG and expressed in different hosts. As a proof-of-principle of using the vector for library screening, a chromophoric Thr65-Tyr-Gly67-stretch of enhanced green fluorescent protein was destroyed and subsequently restored by novel PCR strategy and library screening. The pBVboostFG enables screening of genome-wide libraries, thus making it an efficient new platform technology for functional genomics.

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Section: Discussionmentioning

confidence: 99%

A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo

Laitinen

Airenne²,

Hytönen³

et al. 2005

Nucleic Acids Research

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“…In 1997, Granziero et al (1997) aimed to develop a rapid method for generating baculovirus-based cDNA expression libraries for screening cell surface molecules, for which antibodies are available beforehand and whose expression pattern is restricted to particular cell types. The first proof of principle was gained by cloning a cDNA pool, reverse transcribed from human placenta, into the baculovirus genome and sorting the virus-infected insect cells by flow cytometry using monoclonal antibodies of an unknown specificity as probes.…”

Section: Generation Of Display Librariesmentioning

confidence: 99%

Baculovirus Display: A Multifunctional Technology for Gene Delivery and Eukaryotic Library Development

Mäkelä

Oker‐Blom

2006

Advances in Virus Research

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For over a decade, phage display has proven to be of immense value, allowing selection of a large variety of genes with novel functions from diverse libraries. However, the folding and modification requirements of complex proteins place a severe constraint on the type of protein that can be successfully displayed using this strategy, a restriction that could be resolved by similarly engineering a eukaryotic virus for display purposes. The quite recently established eukaryotic molecular biology tool, the baculovirus display vector system (BDVS), allows combination of genotype with phenotype and thereby enables presentation of eukaryotic proteins on the viral envelope or capsid. Data have shown that the baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), is a versatile tool for eukaryotic virus display. Insertion of heterologous peptides and/or proteins into the viral surface by utilizing the major envelope glycoprotein gp64, or foreign membrane-derived counterparts, allows incorporation of the sequence of interest onto the surface of infected cells and virus particles. A number of strategies are being investigated in order to further develop the display capabilities of AcMNPV and improve the complexity of a library that may be accommodated. Numerous expression vectors for various approaches of surface display have already been developed. Further improvement of both insertion and selection strategies toward development of a refined tool for use in the creation of useful eukaryotic libraries is, however, needed. Here, the status of baculovirus display with respect to alteration of virus tropism, antigen presentation, transgene expression in mammalian cells, and development of eukaryotic libraries will be reviewed.

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“…(2) The number of primary clones from cDNA library can be generally > 10 6 . Besides this, the cDNA library can be cloned using standard procedures and does not involve the in vivo homologous recombinantion process, which is the necessary case for DNA-viruses, such as vaccinia-, baculo-, or adenovirus [Wong et al, 1994;Granziero et al, 1997;He et al, 1998]. (3) The alphaviruses have broad range of host cells including insect, mammalian, or primary cell cultures.…”

Section: Eukaryotic Systemsmentioning

confidence: 99%

Applications of display technologies to proteomic analyses

2001

J. Cell. Biochem.

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With the rapid accumulation of genetic information, development of general experimental approach suitable for large scale annotation and pro®ling of the whole proteome have become one of the major challenges in postgenomic era. Biomolecular display technologies, which allow expressing of a large pool of modularly coded biomolecules, are extremely useful for accessing and analyzing protein diversity and interaction pro®le on a large scale. Recent advances in protein display technologies and their applications to proteomic analyses have been discussed.

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Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

Cited by 17 publications

References 17 publications

A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo

A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo

Baculovirus Display: A Multifunctional Technology for Gene Delivery and Eukaryotic Library Development

Applications of display technologies to proteomic analyses

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