Baculovirus Expression of Chimeric Hepatitis B Virus Core Particles with Hepatitis E Virus Epitopes and Their Use in a Hepatitis E Immunoassay

Touzé, Antoine; Enogat, Nathalie; Buisson, Y.; Coursaget, Pierre

doi:10.1128/jcm.37.2.438-441.1999

Cited by 24 publications

(8 citation statements)

References 13 publications

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“…VLPs closely fit an ideal immunogen. One promising application for the production of non-pathogenic VLPs is to act as most effective immunogens to induce protective immunity and to develop immunogen-based assays [23][24][25]. Another possible application is to define the possible packaging signal of SARS CoV.…”

Section: The S Protein Was Located At the Periphery Of Vlpsmentioning

confidence: 99%

Assembly of human severe acute respiratory syndrome coronavirus-like particles

Lin

Liu

et al. 2004

Biochemical and Biophysical Research Communications

114

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Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.

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Section: The S Protein Was Located At the Periphery Of Vlpsmentioning

confidence: 99%

Assembly of human severe acute respiratory syndrome coronavirus-like particles

Lin

Liu

et al. 2004

Biochemical and Biophysical Research Communications

114

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“…Complexes obtained by direct interaction (see below) were analyzed by electrophoresis on 1% agarose gel followed by ethidium bromide staining. Hepatitis B core particles obtained by expression in insect cells of the Core gene deleted of its 39 C-terminal amino acids (HBcv) was used as control [23].…”

Section: Determination Of Dna Binding Activitymentioning

confidence: 99%

Gene transfer using human papillomavirus pseudovirions varies according to virus genotype and requires cell surface heparan sulfate

Combita

2001

FEMS Microbiology Letters

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Artificial viruses consisting of DNA plasmid packaged in vitro into virus-like particles (VLPs) are new vehicles for gene transfer. We therefore investigated the ability of nine human papillomavirus (HPV) VLPs to interact with heterologous DNA and transfer genes. HPV 16,18, 31, 33, 39, 45, 58, 59, and 68 VLPs were able to bind heterologous DNA and to transfer genes into Cos-7 cells. Inhibition of gene transfer by preincubation of the pseudovirions with heparin confirmed that heparan sulfate on the cell surface plays a role as cell receptor for HPVs. As HPV neutralizing antibodies are mainly type-specific, gene transfer with different HPV pseudovirions offers the possibility of their sequential use in vivo for a greater efficacy. ß

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“…Thus HBcAg has opened the path to the development of more potent therapeutic and preventive vaccines against HBV infection. In addition, HBcAg has become a favourite choice as immunogen carrier for various foreign epitopes [7,8] owing to its ability to self‐assemble in homologous and heterologous expression systems [9].…”

Section: Introductionmentioning

confidence: 99%

The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous‐flow bead milling

Tan

Kamaruddin

et al. 2008

Biotech and App Biochem

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HBcAg (hepatitis B core antigen) is a nanoplex bioproduct that has a great potential in the development of therapeutic drugs and vaccines. In the present study, a continuous-flow bead milling for the disruption of Escherichia coli was optimized and a direct recovery protocol to isolate the recombinant HBcAg from the unclarified E. coli disruptate was developed. The optimal condition for continuous-flow bead milling for the release of HBcAg from E. coli was achieved at a feed flow rate of 15 litres/h, biomass concentration of 10% [ww/v (wet weight/vol.)] and impeller tip speed of 14 m/s. The sucrose-density-gradient analysis showed that the particulate form of the HBcAg released by this optimal condition is still preserved. In the direct purification of HBcAg from the unclarified disruptate, the AE-EBAC (anion-exchange expanded-bed adsorption chromatography) technique was employed. A 54% adsorption and 50.7% recovery of HBcAg were achieved in this direct recovery process. The purity of HBcAg recovered was 49.8%, which corresponds to a purification factor of 2.0. ELISA showed that the HBcAg recovered is functionally active.

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Baculovirus Expression of Chimeric Hepatitis B Virus Core Particles with Hepatitis E Virus Epitopes and Their Use in a Hepatitis E Immunoassay

Cited by 24 publications

References 13 publications

Assembly of human severe acute respiratory syndrome coronavirus-like particles

Assembly of human severe acute respiratory syndrome coronavirus-like particles

Gene transfer using human papillomavirus pseudovirions varies according to virus genotype and requires cell surface heparan sulfate

The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous‐flow bead milling

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