BADH-NAD+-K+ Complex Interaction Studies Reveal a New Possible Mechanism between Potassium and Glutamic 254 at the Coenzyme Binding Site

A comparative study of two aldehyde dehydrogenases from Sphingobium sp.: the substrate spectrum and catalytic mechanism

“…A possible mechanism was proposed whereby high K + concentrations could influence the chemical environment in the catalytic center, leading to increased affinity for NAD + . 24,25 Initially, addition of 500 mM KCl was attempted. The activity of SpALDH2 remained unchanged while the activity of SpALDH1 increased by approximately 5-fold, indicating that SpALDH1 is K + -activated.…”