BAF complexes and the glucocorticoid receptor in breast cancers

Dietrich, Nicholas; Hoffman, Jackson A.; Archer, Trevor

doi:10.1016/j.coemr.2020.07.001

Cited by 8 publications

(5 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synthetic glucocorticoids such as dexamethasone (Dex) are widely prescribed to treat a variety of human diseases, including auto-immune disorders, asthma, cancer, and COVID-19. In human cells, glucocorticoid exposure elicits a rapid and robust transcriptional response mediated by the glucocorticoid receptor (GR) (Dietrich et al, 2020;Miranda et al, 2013;Weikum et al, 2017). For instance, in a variety of cancer cell lines, Dex treatment triggers GR binding at tens of thousands of GR binding sites (GBSs) throughout the genome and alters the expression of hundreds to thousands of genes (Hoffman et al, 2018(Hoffman et al, , 2020Mcdowell et al, 2018;Thomas-Chollier et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Multimodal regulatory elements within a hormone-specific super enhancer control a heterogeneous transcriptional response

et al. 2022

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Multimodal regulatory elements within a hormone-specific super enhancer control a heterogeneous transcriptional response

et al. 2022

Self Cite

View full text Add to dashboard Cite

“…PR is a well-established breast cancer biomarker in evaluating prognosis and response to treatment. GR plays key roles in breast cancer metastasis and response to treatment [ 38 ]. The Cancer Genome Atlas Program (TCGA) analysis reveals high frequency of BRG1 overexpression, but low frequency of BRG1 mutation, in invasive breast cancer [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

USP22 overexpression fails to augment tumor formation in MMTV-ERBB2 mice but loss of function impacts MMTV promoter activity

Kuang,

Salinger,

Benavides

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

The Ubiquitin Specific Peptidase 22 (USP22), a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) histone modifying complex, is overexpressed in multiple human cancers, but how USP22 impacts tumorigenesis is not clear. We reported previously that Usp22 loss in mice impacts execution of several signaling pathways driven by growth factor receptors such as erythroblastic oncogene B b2 (ERBB2). To determine whether changes in USP22 expression affects ERBB2-driven tumorigenesis, we introduced conditional overexpression or deletion alleles of Usp22 into mice bearing the Mouse mammary tumor virus-Neu-Ires-Cre (MMTV-NIC) transgene, which drives both rat ERBB2/NEU expression and Cre recombinase activity from the MMTV promoter resulting in mammary tumor formation. We found that USP22 overexpression in mammary glands did not further enhance primary tumorigenesis in MMTV-NIC female mice, but increased lung metastases were observed. However, deletion of Usp22 significantly decreased tumor burden and increased survival of MMTV-NIC mice. These effects were associated with markedly decreased levels of both Erbb2 mRNA and protein, indicating Usp22 loss impacts MMTV promoter activity. Usp22 loss had no impact on ERBB2 expression in other settings, including MCF10A cells bearing a Cytomegalovirus (CMV)—driven ERBB2 transgene or in human epidermal growth factor receptor 2 (HER2)+ human SKBR3 and HCC1953 cells. Decreased activity of the MMTV promoter in MMTV-NIC mice correlated with decreased expression of known regulatory factors, including the glucocorticoid receptor (GR), the progesterone receptor (PR), and the chromatin remodeling factor Brahma-related gene-1 (BRG1). Together our findings indicate that increased expression of USP22 does not augment the activity of an activated ERBB2/NEU transgene but impacts of Usp22 loss on tumorigenesis cannot be assessed in this model due to unexpected effects on MMTV-driven Erbb2/Neu expression.

show abstract

“…BAF and PBAF complexes, each with unique subunits which dictate their precise function, are recruited to TRβ binding sites within promoters and likely contribute directly to the changes in chromatin accessibility and recruitment of other transcription factors to alter target gene expression. Both BAF and PBAF complexes have been implicated previously in hormone-dependent gene regulation ( 42 , 43 ), however the distinct functional role of each when they are recruited by a nuclear receptor to the same location remains unclear. BAF complexes are preferentially recruited by TRβ to binding sites that occur in distal regulatory regions.…”

Section: Discussionmentioning

confidence: 99%

Thyroid hormone dependent transcriptional programming by TRβ requires SWI/SNF chromatin remodelers

Gillis

Boyd

Tomczak

et al. 2022

Nucleic Acids Research

View full text Add to dashboard Cite

Transcriptional regulation in response to thyroid hormone (3,5,3′-triiodo-l-thyronine, T3) is a dynamic and cell-type specific process that maintains cellular homeostasis and identity in all tissues. However, our understanding of the mechanisms of thyroid hormone receptor (TR) actions at the molecular level are actively being refined. We used an integrated genomics approach to profile and characterize the cistrome of TRβ, map changes in chromatin accessibility, and capture the transcriptomic changes in response to T3 in normal human thyroid cells. There are significant shifts in TRβ genomic occupancy in response to T3, which are associated with differential chromatin accessibility, and differential recruitment of SWI/SNF chromatin remodelers. We further demonstrate selective recruitment of BAF and PBAF SWI/SNF complexes to TRβ binding sites, revealing novel differential functions in regulating chromatin accessibility and gene expression. Our findings highlight three distinct modes of TRβ interaction with chromatin and coordination of coregulator activity.

show abstract

BAF complexes and the glucocorticoid receptor in breast cancers

Cited by 8 publications

References 60 publications

Multimodal regulatory elements within a hormone-specific super enhancer control a heterogeneous transcriptional response

Multimodal regulatory elements within a hormone-specific super enhancer control a heterogeneous transcriptional response

USP22 overexpression fails to augment tumor formation in MMTV-ERBB2 mice but loss of function impacts MMTV promoter activity

Thyroid hormone dependent transcriptional programming by TRβ requires SWI/SNF chromatin remodelers

Contact Info

Product

Resources

About