Baicalein protect pancreatic injury in rats with severe acute pancreatitis by inhibiting pro-inflammatory cytokines expression

Li, Jun; Wu, Yongtao; Zhang, Shu; Zhang, Jian; Ji, Fanpu; Bo, Wangjun; Guo, Xiaoyan; Li, Zongfang

doi:10.1016/j.bbrc.2015.09.094

Cited by 21 publications

(20 citation statements)

References 24 publications

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“…Previous studied showed that baicalein, an active compound isolated from S. baicalensis Georgi, exert anti‐inflammatory, and anti‐tumor effects on CP and PDAC. Baicalein was shown to inhibit the inflammatory response of acinar cells and macrophages in vivo and in vitro (Li et al, ; Zhou et al, ). Here we report results from in vitro experiments aimed at characterizing the effects of baicalein on ADM in pancreatic acinar cells induced by rTNFα and macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…Baicalein (5, 6, 7‐trihydroxy‐2‐phenyl‐4H‐1‐benzopyran‐4‐one), one of its derived product, is a flavonoid with a defined chemical structure, reported to possess excellent anti‐inflammatory, and anti‐tumor activities (Gao, Snyder, Smith, & Chen, ). Baicalein could alleviate experimental pancreatitis by inhibiting the expression of pancreatic enzyme and cytokines via multiple pathways (Li et al, , b). To the best of our knowledge, there are only a few previous reports on the effects of baicalein on pancreatic ADM mainly focused on its effect on apoptosis and proliferation (Cathcart et al, ; Chen et al, ; Guo et al, ; He et al, ; Lian et al, ; Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Baicalein inhibits acinar‐to‐ductal metaplasia of pancreatic acinal cell AR42J via improving the inflammatory microenvironment

Luo

Bai

et al. 2018

Journal Cellular Physiology

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Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive cancers. Recent research has demonstrated that chronic pancreatitis (CP) is associated with an increased risk of PDAC, partly due to acinar-to-ductal metaplasia (ADM). Baicalein has been shown to exert anti-inflammatory and anti-tumor effects for CP or PDAC, respectively. The aim of our study was to investigate the effect of baicalein, and the putative underlying mechanism, on inflammatory cytokines-induced ADM of rat pancreatic acinar cell line AR42J. To investigate ADM and baicalein effects in vitro, AR42J were treated with recombinant rat Tumor Necrosis Factor alpha (rTNFα) with or without baicalein for 5 days. Results showed that rTNFα-induced AR42J cells switched their phenotype from dominantly amylase-positive acinar cells to dominantly cytokeratin 19-positive ductal cells. Moreover, expression of the transcripts for TNFα or Hes-1, a Notch target, was up-regulated in these cells. Interestingly, baicalein reduced the population of ADM as well as cytokines gene expression but not Hes-1. Baicalein inhibited NF-κB activation induced by rTNFα in AR42J, but no effect on Notch 1activation. Moreover, baicalein suppressed the secretion of TNFα and Nitric Oxide (NO) in macrophages stimulated with LPS and further inhibited ADM of conditional medium-treated AR42J cells. Baicalein also suppressed the inflammatory response of LPS-activated macrophages, thereby inhibited ADM of AR42J by altering their microenvironment. Taken together, our study indicates that baicalein reduces rTNFα-induced ADM of AR42J cells by inhibiting NF-κB activation. It also sheds new light on Chinese material medica therapy of pancreatitis and thereby prevention of PDAC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Baicalein inhibits acinar‐to‐ductal metaplasia of pancreatic acinal cell AR42J via improving the inflammatory microenvironment

Luo

Bai

et al. 2018

Journal Cellular Physiology

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“…The inflammatory processes were not part of this study and we could not confirm an anti-inflammatory effect after s.c. injection of baicalein as it was shown after experimental traumatic brain injury [67]. Therefore, it is advisable to use an alternative route of administration, such as orally [50, 68] or intravenously [69]. …”

Section: Discussionmentioning

confidence: 99%

Effect of the Lipoxygenase Inhibitor Baicalein on Muscles in Ovariectomized Rats

Saul

Kling

Kosinsky

et al. 2016

Journal of Nutrition and Metabolism

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Sarcopenia, a loss of muscle mass accompanying osteoporosis, leads to falls and fall-related injuries. Baicalein, as a phytochemical agent, has an antioxidative and anti-inflammatory effect in muscle. In this study, sixty-one female Sprague Dawley rats were divided into five groups: four groups were ovariectomized (OVX) and one control group was nonovariectomized (NON-OVX). Eight weeks after ovariectomy, three disparate concentrations (1 mg/kg body weight (BW), 10 mg/kg BW, and 100 mg/kg BW) of baicalein were applied subcutaneously daily in three OVX groups. Mm. soleus, gastrocnemius, and longissimus were extracted; their diameter, area, relation to body, and muscle weights as well as number of capillaries per fibre were recorded. In Mm. soleus and gastrocnemius, the baicalein effect (increasing number of capillaries per fibre) was proportional to the dose applied. The fibre diameters and area under baicalein treatment were significantly greater compared to OVX and NON-OVX groups. In M. longissimus, we observed a shift to type IIa fibres. Serum creatine kinase levels were significantly lower in highest baicalein concentration group. We conclude that baicalein can stimulate angiogenesis, though not fibre type-specific, in skeletal muscle and reduce the estrogen-related loss of fibre diameter and area in the skeletal muscle in rats. Therefore, a protective effect of baicalein on muscle cells can be assumed.

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“…However, excessive inflammatory reactions result in severe acute pancreatitis (SAP), with a high mortality and morbidity [13]. Although the pathogenesis of SAP has not yet been clarified, inflammatory mediators such as IL-1 β , IL-6, IL-8, IL-10, tumor necrosis factor-alpha (TNF)- α , platelet-activating factor, and monocyte chemotactic protein-1 (MCP-1) are considered vital for SAP development [38]. In vitro, glibenclamide significantly reduced IL-1 β release in lipopolysaccharide- (LPS-) induced peritoneal cells [13].…”

Section: Anti-inflammatory Roles Of Glibenclamidementioning

confidence: 99%

A Protective Role of Glibenclamide in Inflammation-Associated Injury

Zhang

Lin

Zhang

et al. 2017

Mediators of Inflammation

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Glibenclamide is the most widely used sulfonylurea drug for the treatment of type 2 diabetes mellitus (DM). Recent studies have suggested that glibenclamide reduced adverse neuroinflammation and improved behavioral outcomes following central nervous system (CNS) injury. We reviewed glibenclamide's anti-inflammatory effects: abundant evidences have shown that glibenclamide exerted an anti-inflammatory effect in respiratory, digestive, urological, cardiological, and CNS diseases, as well as in ischemia-reperfusion injury. Glibenclamide might block KATP channel, Sur1-Trpm4 channel, and NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasome activation, decrease the production of proinflammatory mediators (TNF-α, IL-1β, and reactive oxygen species), and suppress the accumulation of inflammatory cells. Glibenclamide's anti-inflammation warrants further investigation.

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Baicalein protect pancreatic injury in rats with severe acute pancreatitis by inhibiting pro-inflammatory cytokines expression

Abstract: Baicalein demonstrated a well curative capability on rats with SAP. The mechanism may be alleviateing pancreatic injury and inhibiting pro-inflammatory cytokines expression.

Cited by 21 publications

References 24 publications

Baicalein inhibits acinar‐to‐ductal metaplasia of pancreatic acinal cell AR42J via improving the inflammatory microenvironment

Baicalein inhibits acinar‐to‐ductal metaplasia of pancreatic acinal cell AR42J via improving the inflammatory microenvironment

Effect of the Lipoxygenase Inhibitor Baicalein on Muscles in Ovariectomized Rats

A Protective Role of Glibenclamide in Inflammation-Associated Injury

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