Baicalin increases developmental competence of mouse embryos &lt;i&gt;in vitro&lt;/i&gt; by inhibiting cellular apoptosis and modulating &lt;i&gt;HSP70&lt;/i&gt; and DNMT expression

Qi, Xiaonan; Li, Huatao; Xia, Cong; Wang, Xin; Jiang, Zhongling; Cao, Rongfeng; Tian, Wenru

doi:10.1262/jrd.2016-047

Cited by 14 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When compared with in vivo conditions, the mouse embryo quality and blastocyst development rate are significantly decreased when cultured in vitro. The addition of Baicalin significantly improved the development ability of mouse embryos in vitro, which was associated with the effects of inhibiting apoptosis and reducing cellular stress by Baicalin [ 32 ]. Guo et al (2019) found that Baicalin could inhibit the production of ROS, reduce oocyte apoptosis, and improve the maturity of porcine oocytes and subsequent embryonic development in vitro [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Baicalin improves the functions of granulosa cells and the ovary in aged mice through the mTOR signaling pathway

Fan

Bai

et al. 2022

J Ovarian Res

View full text Add to dashboard Cite

Background The mammalian follicle is the basic functional unit of the ovary, and its normal development is required to obtaining oocytes capable of fertilization. As women get older or decline in ovarian function due to certain pathological factors, the growth and development of follicles becomes abnormal, which ultimately leads to infertility and other related female diseases. Kuntai capsules are currently used in clinical practice to improve ovarian function, and they contain the natural compound Baicalin, which is a natural compound with important biological activities. At present, the role and mechanism of Baicalin in the development of ovarian follicles is unclear. Methods Human primary granulosa cells collected from follicular fluid, and then cultured and treated with Baicalin or its normal control, assessed for viability, subjected to RT-PCR, western blotting, flow cytometry, and hormone analyses. The estrus cycle and oocytes of CD-1 mice were studied after Baicalin administration and compared with controls. Ovaries were collected from the mice and subjected to hematoxylin-eosin staining and immunohistochemistry analysis. Results We showed that Baicalin had a dose-dependent effect on granulosa cells cultured in vitro. A low concentration of Baicalin (for example, 10 μM) helped to maintain the viability of granulosa cells; however, at a concentration exceeding 50 μM, it exerted a toxic effect. A low concentration significantly improved the viability of granulosa cells and inhibited cell apoptosis, which may be related to the resultant upregulation of Bcl-2 expression and downregulation of Bax and Caspase 3. By constructing a hydrogen peroxide-induced cell oxidative stress damage model, we found that Baicalin reversed the cell damage caused by hydrogen peroxide. In addition, Baicalin increased the secretion of estradiol and progesterone by upregulating P450arom and stAR. The results of the in vivo experiment showed that the intragastric administration of Baicalin to aged mice improved the estrous cycle and oocyte quality. Furthermore, we observed that Baicalin enhanced the viability of granulosa cells through the mTOR pathway, which in turn improve ovarian function. Conclusion These results indicate that Baicalin could improve the viability of ovarian granulosa cells and the secretion of steroid hormones and thus could help to improve degenerating ovarian function and delay ovarian aging.

show abstract

Section: Discussionmentioning

confidence: 99%

Baicalin improves the functions of granulosa cells and the ovary in aged mice through the mTOR signaling pathway

Fan

Bai

et al. 2022

J Ovarian Res

View full text Add to dashboard Cite

show abstract

“…Furthremore, baicalin exhibited embryo-protection (Qi et al. 2016), weight losing (Yun and Jung 2014), sleep–wake regulation (Chang et al. 2011), anti-allergic (Kim et al.…”

Section: Pharmacological Activities Of Extracts and Major Compoundsmentioning

confidence: 99%

A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis ofScutellaria baicalensis

Wang

Kuang

et al. 2018

Pharmaceutical Biology

295

178

View full text Add to dashboard Cite

Context:Scutellaria baicalensis Georgi (Lamiaceae) is a popular medicinal plant. Its roots are used as the famous traditional Chinese medicine Huang-Qin, which is recorded in Chinese Pharmacopoeia, European Pharmacopoeia, and British Pharmacopoeia.Objective: This review comprehensively summarizes research progress in phytochemistry, pharmacology, and flavonoid biosynthesis of S. baicalensis.Methods: English and Chinese literature from 1973 to March 2018 was collected from databases including Web of Science, SciFinder, PubMed, Elsevier, Baidu Scholar (Chinese), and CNKI (Chinese). Scutellaria baicalensis, chemical constituents, phytochemistry, biological activities, and biosynthesis were used as the key words.Results: A total of 126 small molecules (1–126) and 6 polysaccharides have been isolated from S. baicalensis. The small molecules can be classified into four structural types, namely, free flavonoids, flavonoid glycosides, phenylethanoid glycosides, and other small molecules. Extracts of S. baicalensis and its major chemical constituents have been reported to possess anti-viral, anti-tumor, anti-bacterial, antioxidant, anti-inflammatory, hepatoprotective, and neuroprotective activities. Key steps in the biosynthetic pathways of Scutellaria flavonoids have also been summarized.Conclusions: This article could be helpful for researchers who are interested in the chemical constituents, bioactivities, biosynthesis, and clinical applications of S. baicalensis.

show abstract

“…It can also improve lung function 6 and fertility 7 .The most common mechanism of Baicalin being against pathogenesis is its protective effect by reducing the production of oxidative stress 8 . Interestingly, Qi et al 9 reported that Baicalin could enhance the developmental competence of mouse embryos through suppressing cellular apoptosis and HSP70 expression, and activating DNA methylation. However, whether or not Baicalin can prevent malformation of the cardiovascular system caused by environmental hazards at an early stage remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Baicalin administration attenuates hyperglycemia-induced malformation of cardiovascular system

et al. 2018

View full text Add to dashboard Cite

In this study, the effects of Baicalin on the hyperglycemia-induced cardiovascular malformation during embryo development were investigated. Using early chick embryos, an optimal concentration of Baicalin (6 μM) was identified which could prevent hyperglycemia-induced cardiovascular malformation of embryos. Hyperglycemia-enhanced cell apoptosis was reduced in embryos and HUVECs in the presence of Baicalin. Hyperglycemia-induced excessive ROS production was inhibited when Baicalin was administered. Analyses of SOD, GSH-Px, MQAE and GABAA suggested Baicalin plays an antioxidant role in chick embryos possibly through suppression of outwardly rectifying Cl(−) in the high-glucose microenvironment. In addition, hyperglycemia-enhanced autophagy fell in the presence of Baicalin, through affecting the ubiquitin of p62 and accelerating autophagy flux. Both Baicalin and Vitamin C could decrease apoptosis, but CQ did not, suggesting autophagy to be a protective function on the cell survival. In mice, Baicalin reduced the elevated blood glucose level caused by streptozotocin (STZ). Taken together, these data suggest that hyperglycemia-induced embryonic cardiovascular malformation can be attenuated by Baicalin administration through suppressing the excessive production of ROS and autophagy. Baicalin could be a potential candidate drug for women suffering from gestational diabetes mellitus.

show abstract

Baicalin increases developmental competence of mouse embryos <i>in vitro</i> by inhibiting cellular apoptosis and modulating <i>HSP70</i> and DNMT expression

Cited by 14 publications

References 44 publications

Baicalin improves the functions of granulosa cells and the ovary in aged mice through the mTOR signaling pathway

Baicalin improves the functions of granulosa cells and the ovary in aged mice through the mTOR signaling pathway

A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis ofScutellaria baicalensis

Baicalin administration attenuates hyperglycemia-induced malformation of cardiovascular system

Contact Info

Product

Resources

About