Baicalin Inhibits Haemophilus Parasuis-Induced High-Mobility Group Box 1 Release during Inflammation

Fu, Shulin; Liu, Huashan; Xiao, Chen; Qiu, Yinsheng; Yuan, Chun; Liu, Yu; Wu, Zhongyuan; Li-wei, Guo; Hou, Yongqing; Hu, Chien‐An Andy

doi:10.3390/ijms19051307

Cited by 12 publications

(8 citation statements)

References 61 publications

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“…Baicalin is used clinically in humans and animals because of its antimicrobial, anti-inflammatory, antitumor and antioxidant properties [ 21 , 22 , 23 , 24 ]. Our previously work demonstrated that baicalin has anti–inflammatory effects in G. parasuis -challenged piglets in vivo and in vitro by suppressing inflammatory cytokines and HMGB1 via NF-κB and NLRP3 signaling and reversing apoptosis by altering PKC–MAPK signaling cells [ 25 , 26 , 27 , 28 ]. Baicalin is prominent in the literature on protection of tight junction in epithelial and endothelial cells [ 17 , 29 , 30 ], although high doses of baicalin can reduce tight junction integrity by partly targeting the first PDZ domain of ZO-1 [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Zhang

et al. 2021

Molecules

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Glaesserella parasuis (G. parasuis) causes inflammation and damage to piglets. Whether polyserositis caused by G. parasuis is due to tight junctions damage and the protective effect of baicalin on it have not been examined. Therefore, this study aims to investigate the effects of baicalin on peritoneal tight junctions of piglets challenged with G. parasuis and its underlying molecular mechanisms. Piglets were challenged with G. parasuis and treated with or without baicalin. RT-PCR was performed to examine the expression of peritoneal tight junctions genes. Immunofluorescence was carried out to detect the distribution patterns of tight junctions proteins. Western blot assays were carried out to determine the involved signaling pathways. Our data showed that G. parasuis infection can down-regulate the tight junctions expression and disrupt the distribution of tight junctions proteins. Baicalin can alleviate the down-regulation of tight junctions mRNA in peritoneum, prevent the abnormalities and maintain the continuous organization of tight junctions. Our results provide novel evidence to support that baicalin has the capacity to protect peritoneal tight junctions from G. parasuis-induced inflammation. The protective mechanisms of baicalin could be associated with inhibition of the activation of PKC and MLCK/MLC signaling pathway. Taken together, these data demonstrated that baicalin is a promising natural agent for the prevention and treatment of G. parasuis infection.

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Section: Introductionmentioning

confidence: 99%

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Zhang

et al. 2021

Molecules

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“…HMGB1 also participates in systemic fibrotic diseases through the RAGE/MAPK and NF-κB signalling pathways [ 42 ]. In a previous study, we showed that G. parasuis stimulated the release of HMGB1 in piglet PBMNs [ 22 ] and induced the activation of the RAGE and MAPK signalling pathways in porcine aortic vascular endothelial cells [ 20 ]. Therefore, we investigated whether HMGB1 is released and the MAPK signalling pathway is activated in piglets after challenge with G. parasuis .…”

Section: Discussionmentioning

confidence: 99%

“…Baicalin reduced apoptosis triggered by G. parasuis via RAGE, MAPK, and AP-1 in PAVECs [ 20 ]. Baicalin also inhibited PKC-MAPK signalling pathway activation [ 21 ] and attenuated high-mobility group box 1 (HMGB1) secretion [ 22 ] in PMNPs stimulated by G. parasuis . In addition, baicalin could modulate long non-coding RNA and mRNA expression in PAVECs infected by G. parasuis [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

The effects of baicalin on piglets challenged with Glaesserella parasuis

Yin

Zuo

et al. 2020

Vet Res

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Glaesserella parasuis ( G. parasuis ) causes porcine vascular inflammation and damage. Baicalin is reported to have antioxidant and anti-inflammatory functions. However, whether baicalin protects piglets against G. parasuis challenge and the potential protective mechanism have not been investigated. Therefore, in this study, we comprehensively examined the protective efficacy of baicalin in piglets challenged with G. parasuis and the possible protective mechanism. Our results show that baicalin attenuated the release of the inflammation-related cytokines interleukin (IL) 1β, IL6, IL8, IL10, and tumour necrosis factor α (TNF-α) and reduced high mobility group box 1 (HMGB1) production and cell apoptosis in piglets infected with G. parasuis . Baicalin also inhibited the activation of the mitogen-activated protein kinase (MAPK) signalling pathway and protected piglets against G. parasuis challenge. Taken together, our data suggest that baicalin could protect piglets from G. parasuis by reducing HMGB1 release, attenuating cell apoptosis, and inhibiting MAPK signalling activation, thereby alleviating the inflammatory response induced by the bacteria. Our results suggest that baicalin has utility as a novel therapeutic drug to control G. parasuis infection.

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“…We have previously demonstrated that the anti‐inflammatory effects of baicalin are mediated through the suppression of the NLRP3 inflammasome pathway in a lipopolysaccharide (LPS)‐challenged piglet mononuclear phagocyte model (Ye et al, ). Baicalin also suppresses the secretion of the inflammatory cytokines and HMGB1 via NF‐κB and NLRP3 signaling in Haemophilus parasuis (HPS)‐stimulated piglet cells (Fu, Liu, Chen, et al, ; Fu, Liu, Xu, et al, ; Fu et al, ). Furthermore, baicalin can reverse apoptosis initiated by HPS by altering PKC–MAPK signaling (Ye et al, ).…”

Section: Pharmacokinetic Parameters Of Baicalin Following Single Intrmentioning

confidence: 99%

Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets

Liu

Zhao

et al. 2019

Vet Pharm & Therapeutics

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The purpose of this study was to determine the pharmacokinetics of baicalin after intravenous and intramuscular administration of sodium baicalin at 50 mg/kg to piglets. Plasma baicalin levels were determined by high‐performance liquid chromatography. The plasma concentration–time data of baicalin for both administration routes were best described by two‐compartmental open model. The area under the plasma concentration–time curve and the elimination half‐lives were 77.47 ± 6.14 µg/ml × h and 1.73 ± 0.16 hr for intravenous and 64.85 ± 5.67 µg/ml × h and 2.42 ± 0.15 hr for intramuscular administration, respectively. The apparent volume of distribution and body clearance were 1.63 ± 0.23 L/kg and 2.74 ± 0.30 L h−1 kg−1 for intravenous and 0.51 ± 0.10 L/kg and 0.78 ± 0.08 L h−1 kg−1 for intramuscular routes, respectively. An intramuscular injection of sodium baicalin in piglets resulted in rapid and complete absorption, with a mean maximal plasma concentration of 77.28 ± 7.40 µg/ml at 0.17 hr and a high absolute bioavailability of 83.73 ± 5.53%.

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Baicalin Inhibits Haemophilus Parasuis-Induced High-Mobility Group Box 1 Release during Inflammation

Cited by 12 publications

References 61 publications

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

The effects of baicalin on piglets challenged with Glaesserella parasuis

Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets

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