Bak is a key molecule in apoptosis induced by methanol extracts of Codonopsis lanceolata and Tricholoma matsutake in HSC-2 human oral cancer cells

Shin, Ji‐Ae; Kim, Jun Sung; Hong, In–Sun; Cho, Sung‐Dae

doi:10.3892/ol.2012.898

Cited by 18 publications

(6 citation statements)

References 29 publications

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“…Several types of Codonopsis extracts have shown anticancer activities. Notably a methanolic extract from C. lanceolata was found to inhibit cancer proliferation and to induce apoptosis of HSC-2 human oral cancer cells in vitro, with an increased expression of the protein Bak [ 46 ]. Also, a n-butanol fraction of C. lanceolata was found to dose-dependently inhibit the growth of HT-29 human colon cancer cells, via the production of intracellular reactive oxygen species (ROS) and polyamine depletion, leading to the induction of cell apoptosis [ 47 ].…”

Section: Anticancer Activity Of Codonopsis Extractsmentioning

confidence: 99%

Anticancer Properties of Lobetyolin, an Essential Component of Radix Codonopsis (Dangshen)

Bailly

2020

Nat. Prod. Bioprospect.

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Lobetyolin (LBT) is a polyacetylene glycoside found in diverse medicinal plants but mainly isolated from the roots of Codonopsis pilosula, known as Radix Codonopsis or Dangshen. Twelve traditional Chinese medicinal preparations containing Radix Codonopsis were identified; they are generally used to tonify spleen and lung Qi and occasionally to treat cancer. Here we have reviewed the anticancer properties of Codonopsis extracts, LBT and structural analogs. Lobetyolin and lobetyolinin are the mono- and bis-glucosylated forms of the polyacetylenic compound lobetyol. Lobetyol and LBT have shown activities against several types of cancer (notably gastric cancer) and we examined the molecular basis of their activity. A down-regulation of glutamine metabolism by LBT has been evidenced, contributing to drug-induced apoptosis and tumor growth inhibition. LBT markedly reduces both mRNA and protein expression of the amino acid transporter Alanine-Serine-Cysteine Transporter 2 (ASCT2). Other potential targets are proposed here, based on the structural analogy with other anticancer compounds. LBT and related polyacetylene glycosides should be further considered as potential anticancer agents, but more work is needed to evaluate their efficacy, toxicity, and risk–benefit ratio.

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Section: Anticancer Activity Of Codonopsis Extractsmentioning

confidence: 99%

Anticancer Properties of Lobetyolin, an Essential Component of Radix Codonopsis (Dangshen)

Bailly

2020

Nat. Prod. Bioprospect.

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“…Prior to this investigation, several studies confirmed that steaming and the fermentation process significantly improved the cognitive-enhancement and neuroprotective effects of C. lanceolata [ 7 , 20 , 21 ]. In addition, recent studies have indicated that the extract of C. lanceolata exerted inhibition activities on HSC-2 (Hematopoietic Stem Cell-2) human oral cancer cells and HT-29 human colon cancer cell [ 22 , 23 ]. However, the anti-tumor efficacy in vivo of steamed C .…”

Section: Introductionmentioning

confidence: 99%

Anti-Tumor Effect of Steamed Codonopsis lanceolata in H22 Tumor-Bearing Mice and Its Possible Mechanism

et al. 2015

Nutrients

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Although previous studies confirmed that steaming and the fermentation process could significantly improve the cognitive-enhancement and neuroprotective effects of Codonopsis lanceolata, the anti-tumor efficacy of steamed C. lanceolata (SCL) and what mechanisms are involved remain largely unknown. The present study was designed to evaluate the anti-tumor effect in vivo of SCL in H22 tumor-bearing mice. The results clearly indicated that SCL could not only inhibit the tumor growth, but also prolong the survival time of H22 tumor-bearing mice. Besides, the serum levels of cytokines, such as interferon gamma (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-2 (IL-2), were enhanced by SCL administration. The observations of Hoechst 33258 staining demonstrated that SCL was able to induce tumor cell apoptosis. Finally, immunohistochemical analysis revealed that SCL treatment significantly increased Bax expression and decreased Bcl-2 and vascular endothelial growth factor (VEGF) expression of H22 tumor tissues in a dose-dependent manner. Moreover, LC/MS analysis of SCL indicated that it mainly contained lobetyolin and six saponins. Taken all together, the findings in the present study clearly demonstrated that SCL inhibited the H22 tumor growth in vivo at least partly via improving the immune functions, inducing apoptosis and inhibiting angiogenesis.

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“…The current treatments for oral cancer are inadequate and a significant proportion of oral cancer patients may develop local invasion and metastases (11). Numerous studies have been performed to find novel agents (especial from natural plant) which can trigger programmed cell death (apoptosis) in tumor cells and hopefully to provide a new therapeutic approach for anticancer design (12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Tetrandrine induces cell death in SAS human oral cancer cells through caspase activation-dependent apoptosis and LC3-I and LC3-II activation-dependent autophagy

Huang¹,

Lien

Meng

et al. 2013

International Journal of Oncology

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Abstract. Numerous studies have demonstrated that autophagy is associated with cancer development. Thus, agents to induce autophagy could be employed in some cases for the treatment of cancer. Our results showed that tetrandrine significantly decreased the viability of SAS cells in a concentration-and time-dependent manner. Tetrandrine induced nuclear condensation, demonstrated by DAPI staining. The early events in apoptosis analysed by Annexin V/PI staining indicated that the percentage of cells staining positive for Annexin V was slightly increased in SAS cells with tetrandrine treatment but was much lower following bafilomycin A1 pre-treatment. Tetrandrine caused AVO and MDC induction in SAS cells in a concentration-dependent manner by fluorescence microscopy. Tetrandrine also caused LC-3 expression in SAS cells in a time-dependent manner. Our results show that tetrandrine treatment induced the levels of cleaved caspase-3 in a concentration-and time-dependent manner. Tetrandrine treatment induced the levels of LC-3 II, Atg-5, beclin-1, p-S6, p-ULK, p-mTOR, p-Akt (S473) and raptor. Tetrandrine decreased cell viability, but bafilomycin A1, 3-MA, chloroquine and NAC protected tetrandrine-treated SAS cells against decrease of cell viability. Atg-5, beclin-1 siRNA decreased tetrandrineinduced cleaved caspase-3 and cleaved PARP in SAS cells and protected tetrandrine-treated SAS cells against decrease in cell viability. Chloroquine, NAC and bafilomycin A1 also decreased tetrandrine-induced cleaved caspase-3 and cleaved PARP in SAS cells. Our results indicate the tetrandrine induces apoptosis and autophagy of SAS human cancer cells via caspase-dependent and LC3-I and LC3-II-dependent pathways.

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Bak is a key molecule in apoptosis induced by methanol extracts of Codonopsis lanceolata and Tricholoma matsutake in HSC-2 human oral cancer cells

Cited by 18 publications

References 29 publications

Anticancer Properties of Lobetyolin, an Essential Component of Radix Codonopsis (Dangshen)

Anticancer Properties of Lobetyolin, an Essential Component of Radix Codonopsis (Dangshen)

Anti-Tumor Effect of Steamed Codonopsis lanceolata in H22 Tumor-Bearing Mice and Its Possible Mechanism

Tetrandrine induces cell death in SAS human oral cancer cells through caspase activation-dependent apoptosis and LC3-I and LC3-II activation-dependent autophagy

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