Bakteri Simbion Karang Lunak Sinularia sp. sebagai Agen Antibakteri

Widyaningsih, Sekar; Sa’adah, Nor

doi:10.14710/jkt.v21i1.2454

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…At dilutions 10-6, 10-7, 10-8, 1 ml was taken using a micropipette and inoculated into a petri dish with marine agar media using the spread plate method [20]. The purification of the symbiont bacteria was carried out using the streak plate method with the help of round loops [21]. The pure bacterial culture was transferred to the agar slanting media for marine agar in a test tube as culture stock and working culture.…”

Section: Isolation Of Soft Coral Symbiont Bacteriamentioning

confidence: 99%

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Wulandari,

Amatullah,

Lunggani

et al. 2024

BIO Web Conf.

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Soft corals can produce bioactive compounds that act as antibacterials. Sinularia sp. is a soft coral that can produce antibacterial compounds. Symbion bacteria are bacteria that can produce bioactive compounds that tend to be the same as their host. The use of symbiont bacteria can be an alternative to exploit the potential of Sinularia sp. without harming marine life. This study aims to determine the antibacterial potential of the symbiont bacteria obtained from the soft coral Sinularia sp. in inhibiting the growth of Propionibacterium acnes and Staphylococcus epidermidis. The antibacterial activity test was carried out by the agar diffusion method using disc paper. Antibacterial activity was indicated by the formation of a clear zone around the disc paper. The results showed that LA7 isolate had the best antibacterial activity with an average clear zone size of 14.6 mm against Staphylococcus epidermidis and 16.6 mm against Propionibacterium acnes. LA7 isolates identified species based on the 16S rRNA gene with primers 27F and 1492R. Based on molecular species support, it was found that LA7 isolate is Bacillus aerius with a homology level of 98.17%.

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Section: Isolation Of Soft Coral Symbiont Bacteriamentioning

confidence: 99%

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Wulandari,

Amatullah,

Lunggani

et al. 2024

BIO Web Conf.

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“…Ketersediaan spons yang banyak pada perairan kendit utamanya adalah Ptilocaulis marquezii dapat dimanfaatkan ekstrak maupun bakteri simbionnya sebagai agen antibakteri, berdasarkan Widyaningsih & Sa'adah (2018), metabolit sekunder yang dimiliki organisme inangnya dapat disintesis oleh metabolit sekunder. Berdasarkan pernyataan tersebut bakteri yang bersimbiosis dengan Ptilocaulis marquezii dapat dijadikan sebagai sumber alternatif baru sebagai antibakteri dan juga ekstrak yang berasal dari Ptilocaulis marquezii seperti Aplysina sp.…”

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Uji Ekstrak Spons Laut Jenis Ptilocaulis marquezii dari Perairan Kendit sebagai Potensi Antibakteri Escherichia coli

et al. 2023

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Guna mengetahui potensi bioaktivitas senyawa yang terkandung serta mengetahui uji ekstrak spons Ptilocaulis marquezii terhadap pertumbuhan bakteri Eschercia coli. Penelitian dilakukan secara eksperimen berupa uji ekstrak berupa pembuatan ekstrak spons Ptilocaulis marquezii dengan maserasi serta evaporasi dengan rotary vacuum evaporator pada suhu 40°C hingga ekstrak terbentuk. Dilakukan pengujian dengan metode Difusi cakram atau Kirby-Bauer test. Pengujian fitokimia guna mengetahui kandungan senyawa aktif. Hasil yang diperoleh didapatkan ekstrak dengan konsentrasi 40% yang memiliki kemampuan sedang. Pada uji fitokimia mengandung alkoloid dan triptenoid. Ekstrak spons Ptilocaulis marquezii pada konsentrasi 40% dengan zona hambat sebesar 9.5 mm dalam kategori sedang dan pada konsentrasi lainnya yaitu 10%, 20%, 60% dan 80% berkategori lemah. Senyawa alkaloid menghambat pertumbuhan Escherichia Coli dengan merusak susunan peptidoglycan. Pada senyawa triterpenoid merusak membran plasma pada bakteri Escherichia coli. Knowing the potential bioactivity of the compounds contained and knowing the test of the extract of the Ptilocaulis marquezii sponge of Eschercia coli bacteria. The research was carried out experimentally in the form of extract testing in the form of making Ptilocaulis marquezii sponge extract by maceration and evaporation with a rotary vacuum evaporator at a temperature of 40°C until the extract was formed. The test was carried out using the disc diffusion method or the Kirby-Bauer test. Phytochemical testing to determine the content of active compounds. The results obtained were extracts with a concentration of 40% which had moderate abilities. The phytochemical test contains alkaloids and triptenoids. Ptilocaulis marquezii sponge extract at a concentration of 40% with an inhibition zone of 9.5 mm in the medium category and at other concentrations of 10%, 20%, 60% and 80% in the weak category. Alkaloids inhibit the growth of Escherichia Coli by destroying the peptidoglycan structure. The triterpenoid compounds damage the plasma membrane of Escherichia coli bacteria.

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Symbiont bacteria cultures from the red algae Eucheuma spinosum, isolation of bioactive proteins and their anticancer potential test

Ramadan

Ahmad

Marinda

et al. 2019

J. Phys.: Conf. Ser.

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Red algae Eucheuma spinosum is one of marine organisms which have the potential bioactive protein. This research aimed to determine the protein concentration of red algae Eucheuma spinosum and to discover its potential as an anticancer agent. Protein was isolated from bacterial symbiont of red algae Eucheuma spinosum by buffer A with pH value 8.3. Protein crude extract was fractionated by adding ammonium sulphate with a saturation level of 0-20%, 20-40%, 40-60%, and 60-80%. The result was dialyzed using cellophane membrane. Lowry method with Bovine Serum Albumin (BSA) was used as the standard to determine the protein level. An anticancer preliminary test was conducted using Brine Shrimp Lethality Test (BSLT) method. The result showed that the protein concentration from crude extract of red algae Eucheuma spinosum was 33.325 mg/mL. The highest concentration that was obtained at fraction 0-20% is 32.145 mg/mL. The result of toxicity test using Brine Shrimp Lethality Test (BSLT) method at protein fraction of 20-40% has a very low LC50 value at <1000 µg/mL. Red algae protein fraction is potential to be developed as anticancer agent.

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Bakteri Simbion Karang Lunak Sinularia sp. sebagai Agen Antibakteri

Cited by 4 publications

References 4 publications

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Uji Ekstrak Spons Laut Jenis Ptilocaulis marquezii dari Perairan Kendit sebagai Potensi Antibakteri Escherichia coli

Symbiont bacteria cultures from the red algae Eucheuma spinosum, isolation of bioactive proteins and their anticancer potential test

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