Bakterien in Gallenblasenwand und Gallensteinen ?Indikation zur Cholecystektomie

Hancke, E.; Nusche, A.; Marklein, G.

doi:10.1007/bf01263214

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…First, aspirated bile samples contain floating bacterial species but cannot detect sessile bacterial populations adhering to the mucosa or those residing in the biofilm. However, since a study demonstrated that bacteria in the bile fluid almost always coincided with the presence of bacteria on the bile duct wall [ 31 ], bacteria species floating in bile may sufficiently reflect the total microbial environment of biliary tract. Nevertheless, additional bile duct tissue biopsies can be expected to reveal more accurate microbial communities in the CBD.…”

Section: Discussionmentioning

confidence: 99%

Analysis on Microbial Profiles & Components of Bile in Patients with Recurrent CBD Stones after Endoscopic CBD Stone Removal: A Preliminary Study

Choe

Lee

Hyun

et al. 2021

JCM

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Background/Aim: Common bile duct (CBD) stone recurrence after endoscopic treatment is a major concern as a late complication. Biliary bacterial factors and biochemical factors determine the path of gallstone formation. The aim of this preliminary study was to investigate the microbial profile and components of bile in patients with and without recurrent CBD stones after endoscopic CBD stone removal. Methods: Among patients who had undergone an initial endoscopic procedure for the removal of CBD stones and were followed up for >2 years, 11 patients who experienced at least two CBD stone recurrences, six months after endoscopic retrograde cholangiopancreatography (ERCP), were categorized into the recurrence group. Nine patients without CBD recurrence events were matched. Results: Polymicrobial infections are generally seen in all patients who have biliary sphincteroplasty. Microbial richness, measured by the numbers of operational taxonomic units (OTUs), was reduced in the recurrence group. The microbial evenness was also significantly lower than in the non-recurrence group. The overall microbial communities in the recurrence group deviated from the non-recurrence group. Infection with bacteria exhibiting β-glucuronidase activity was more frequent in the recurrence group, but there was no statistical significance. In an analysis of the bile components, the bile acid concentration was higher in the non-recurrence group than in the recurrence group. However, the other metabolites were not significantly different. Conclusions: Microbiota dysbiosis and altered bacterial community assembly in bile duct and decreased bile acid in bile juice were associated with recurrence of bile duct stone.

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Section: Discussionmentioning

confidence: 99%

Analysis on Microbial Profiles & Components of Bile in Patients with Recurrent CBD Stones after Endoscopic CBD Stone Removal: A Preliminary Study

Choe

Lee

Hyun

et al. 2021

JCM

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“…In a study of E Hancke, 19 after cholecystectomy the bacterial content of the bile, gallbladder wall and gallstones was studied in 40 patients. Bacteria could be found in 9 cases in gallbladder wall, in 8 cases in the gallstones and in 3 cases in the bile.…”

Section: Discussionmentioning

confidence: 99%

The Incidence of Live Bacteria in Bile, Gallbladder Inner Wall and Gallstones in Gallstone Disease- A Search

Mandal¹,

Dheeraj²,

Das³

et al. 2017

jemds

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BACKGROUNDGallstone disease and associated cholecystectomy has taken enormous portion of all surgical procedures in the world, as it is the most frequent surgical procedure practiced in any part of the world. The myth of fat, fertile, female of forty has long been busted as also the story of "Typhoid Mary." As gallstones are now seen in all age groups, in both sexes and in all corners of the globe, most frequently asked question confronted by the surgeons in the clinic from the patients is how these stones occur? Various theories of stone formation have been postulated, but the role of bacteria in gallstone formation is still mysterious.In this study, we are trying to find out the incidence of live bacteria in bile, gallbladder inner wall and gallstones in gallstone disease.

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Time dependent changes in the concentration and type of bacterial sequences found in cholesterol gallstones

et al. 1998

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The role of bacteria in gallstone formation could not be conclusively evaluated until bacterial presence or absence in a stone was consistently shown. Cultural bacteriologic investigations at the time of cholecystectomy, however, led to the assumption that cholesterol gallstones were free of bacteria. In this study, we used a culture independent, molecular genetic approach to detect, quantify, and identify bacteria in cholesterol gallstones from 100 patients at the time of cholecystectomy and 6 months following. Bacterial growth was recorded in the culture in 9 of 100 gallstones; bacterial DNA, however, was detected in 82 of 91 sterile gallstones. High concentrations corresponding to between 10 6 to 10 7 bacteria/g were detected in 11 stones and low concentrations of 10 5 bacteria/g were detected in 71 sterile stones. The infection in stones with a positive bacterial culture was characterized by the predominance of single bacterial sequence(s) of the bacteria cultured. A similar predominance, indicating a recent infection, was found in sterile gallstones with low DNA concentrations. A high diversity of non-repeating bacterial sequences, possibly arising from previous overlapping infections, was found in sterile gallstones with high concentrations of bacterial DNA. After 6 months concentrations of bacterial DNA fell significantly in all groups of gallstones. As bacterial DNA is quickly destroyed upon storage, but is nevertheless readily found in most gallstones at the time of cholecystectomy, there must be a mechanism by which it is replenished. One such mechanism is the frequently reoccurring, possibly self-terminating infection and another one is the permanent colonization of the gallstone with bacteria at low concentrations. Both can promote cholecystolithiasis. (HEPATOLOGY 1998;27:662-665.)Our knowledge about the cholesterol gallstone comes primarily from the investigation of its composition at the time of cholecystectomy and from brief model experiments with saturated bile. Gallstone formation, however, takes years, and the vast majority of events that occur in the time between cholesterol crystal nucleation and their consolidation into a gallstone is not accessible to a direct investigation. Gallstone formation is, therefore, incompletely understood. Because cholesterol gallstones are more than 70% cholesterol and because bacteria are not regularly cultured at the time of cholecystectomy, the role of bacteria in the pathogenesis of cholesterol gallstones has been questioned. 1 Bacteria, however, are a good explanation for the initiation of nucleation. 2 Bacterial polymers are ideal substances for both binding loose cholesterol crystals to a massive gallstone and for building a matrix in which cholesterol later crystallizes. 3 Unfortunately, there is no convincing model to prove either of these hypotheses and the data about the occurrence of bacteria in bile, 4,6-7 the billiary tree, 5,8 and in the gallstones 8 are inconsistent.Bacteriologic findings depend on bacterial vitality and culturability. As bacteri...

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Bakterien in Gallenblasenwand und Gallensteinen ?Indikation zur Cholecystektomie

Cited by 5 publications

References 8 publications

Analysis on Microbial Profiles & Components of Bile in Patients with Recurrent CBD Stones after Endoscopic CBD Stone Removal: A Preliminary Study

Analysis on Microbial Profiles & Components of Bile in Patients with Recurrent CBD Stones after Endoscopic CBD Stone Removal: A Preliminary Study

The Incidence of Live Bacteria in Bile, Gallbladder Inner Wall and Gallstones in Gallstone Disease- A Search

Time dependent changes in the concentration and type of bacterial sequences found in cholesterol gallstones

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