BALB/cAn B cells and T cells have distinct susceptibilities to cytotoxic effects of snake venom

Lewis, G M; Minton, Sherman A.; Slack, J H

doi:10.1016/0041-0101(90)90072-f

Cited by 5 publications

(1 citation statement)

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“…Serine protease and metalloprotease play a major role in the coagulopathy which involves the prothrombin activation (Nicolaes and Dahlback, 2002) and leading to the formation of thrombin and fibrin clots (Mizuno et al, 2001), respectively. L-amino acid oxidase has strong effect against some type of tissue which leads to necrotic and apoptotic cell death (Lewis et al, 1990;Gopalakrishnakone et al, 1995;Du and Clemetson, 2002). C-type lectins show an anti-coagulant activity that contribute to the total coagulopathic activity of venom (Morita, 2005).…”

Section: Chapter II Literature Reviewmentioning

confidence: 99%

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom

Sittishevapark

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The eastern Russell's viper, Daboia siamensis, is one of the important venomous snakes in Thailand. Its venom possesses hematotoxin causing pathological alterations to circulatory and renal systems. Although antivenom serum�is used for standard medical treatment, its cost per dose, ineffectiveness for some symptoms, and potential to develop allergic reactions in patients has called attention to an alternative remedy including the medicinal herb. To find effective herbs, appropriate screening assays are needed. This study aims to develop in vitro and in vivo screening assays and use for screening Thai herbs with anti-hematotoxic activity against D. siamensis venom. For in vitro assay, five assays corresponding to 5 biological activities of venom were developed. Three assays for phospholipase, coagulation, and fibrinogenolytic activities have been successfully developed and effectively showed a marked difference between positive and negative controls. Two assays for proteolytic and hemagglutination activities were proven to be not suitable for the screening because of the low activity in D. siamensis venom. For in vivo assay, an alternative chick embryo assay for hematotoxic activities of D. siamensis venom was developed. The microanatomy of embryonic vasculature was verified as suitable markers for the hematotoxic effect of snake venom. Subsequently, 10 herbal extracts were screened with the validated in vitro assays. The results showed that Areca catechu�L. is the most effective herb with 45.93% inhibition against the venom. Various concentration of A. catechu extract was used to neutralize D. siamensis venom at LD50 concentration (6.35 ?g/?L), and the mixture applied to the chick embryo. It was found that A. catechu extract reduced the mortality of chick embryo in a dose dependent manner. The median inhibitory dose (ID50) of A. catechu against D. siamensis venom in chick embryo during 4-hour exposure was calculated at 4.42 ?g/?L. With the strong anti-hemotoxic effect and the low toxicity of A. catechu (LD50 of 445.16 ?g/?L), this herb should be further developed into the herbal remedy for snakebit treatment.

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Section: Chapter II Literature Reviewmentioning

confidence: 99%

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom

Sittishevapark

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Humoral response following russell's viper (Daboia russelli siamensis) bites in myanmar

Tun-Pe

Aye-Aye-Myint

May-Mya-Win

1995

Toxicon

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Effect of various Viperidae and Crotalidae snake venoms on endothelial cells in vitro

Borkow

Lomonte

Gutiérrez

et al. 1994

Toxicon

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BALB/cAn B cells and T cells have distinct susceptibilities to cytotoxic effects of snake venom

Cited by 5 publications

References 28 publications

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom

Humoral response following russell's viper (Daboia russelli siamensis) bites in myanmar

Effect of various Viperidae and Crotalidae snake venoms on endothelial cells in vitro

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BALB/cAn B cells and T cells have distinct susceptibilities to cytotoxic effects of snake venom

Cited by 5 publications

References 28 publications

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom

Humoral response following russell's viper (Daboia russelli siamensis) bites in myanmar

Effect of various Viperidae and Crotalidae snake venoms on endothelial cells in vitro

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Product

Resources

About

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom

Screening development for anti-hemotoxic activity of Thai herbs against eastern Russells viper Daboia Siamensis (Smith, 1917) venom