First of all, I want to thank my dear mother Gloria, for her love, for believing in me, her wise advice and unconditional support. To my brother Oscar, grandparents, uncles, family, and friends, to all those people who sent me messages of encouragement and energy. To my adviser teacher, Patricia, for the teachings, the motivation, the suggestions and all the contributions to the work. Thanks for all the trust and patience. To the entire Labsem team