Banded karyotype of Nelore cattle (Bos taurus indicus Linnaeus, 1758)

Abstract

Chromosome banding techniques were applied and standardized to obtain karyotype characteristics for the first time in Brazil of Nelore cattle – Bos taurus indicus Linnaeus, 1758 – (bovine subspecies most prominent in Brazilian livestock). Blood samples were collected from the animals of the School of Agrarian and Biological Sciences of the Pontifical Catholic University of Goiás, two males and two females of pure breed. These samples were submitted to the cell culture method to study metaphase chromosomes. Chromosome banding techniques (C, G and NOR) revealed the karyotype architecture of Nelore cattle common with that of other breeds of zebu cattle formerly karyotyped. The diploid chromosome number was invariably normal, 2n = 60. C-banding revealed C-positive heterochromatin in centromeric regions almost in all chromosomes. G-banding presented the expected band pattern in the respective chromosome pairs in correspondence with the established chromosomal patterns for the species. Ag-staining for nucleolus organizer regions (AgNOR) was identified on the telomeric end of the long arm in 7 autosomal chromosomes. In this study we found more regions in chromosomes with staining than presented in the literature for the Bos indicus group (BIN). These NOR regions were repeated on the same chromosomes for the 4 animals studied.

A rare chromosomal polymorphism in a Kangayam bull (Bos indicus) of south India

“…2). This is like the standard CBG-banding pattern of cattle as reported for Red Danish (Hansen 1973), Bos taurus L. (Iannuzzi and Berardino 1985), Jersey crossbreds (Chauhan et al 2009), Tho-Tho cattle (Longkumer et al 2015), mithun (Ezung 2016) and Nellore cattle (Amancio et al 2019). However, the unpaired acrocentric chromosome with a small p-arm also stained darkly throughout the length showing no centromere banding (Fig.…”

“…Red Kandhari (Katkade 2005), Khillari (Nakod 2013), Malnad Gidda (Suresh et al 2015), Indonesian native bulls (Ciptadi et al 2017) and Nellore cattle (Amancio et al 2019). The GTG-banding results also confirmed that there was no translocation between autosomes and Y-chromosome, as all the autosomes were having the typical G-banding pattern, as those of Iannuzzi and Di Meo (1995) and ISCNDB 2000 (Cribiu et al 2001).…”

Comparative analysis of karyotypes of Вovidae family from the evolutionary aspect

“…In our opinion, a relatively high frequency of centric fusions of chromosomes in agricultural animals may be determined on the cellular level by nucleolus organizers since the chromosome sites, used in the processes of centric fusion, are concentrated in these very sites. DISCUSSION Much information has been accumulated within 40 recent years regarding the comparative analysis of dif-ferentiated banded chromosomes of different species of animals which allowed to determine the main tendencies in the karyotype evolution within many taxa (Robinson TJ et al, 2011;Amancio AP et al, 2019). The chromosomal evolution is understood as the processes related to transformations (reformatting) of mutually located sites of genome, which, on the cytological level, results in a change in the number of chromosomes and chromosomal transformations and in a change in the amount of euchromatin in the chromosomes as well as the modifi cation of the number and localization of nucleolus organizers (Ruiz-Herrera A et al, 2006;Qumsiyeh MB, 1999).…”

Karyotype Symmetry/ Asymmetry and Karyotypic Relationships of Cervidae (Artiodactyla) Taxa

“…The karyotypes of genus Alburnus (Rafinesque, 1820) are symmetric in two species, and between symmetric and asymmetric in five species (Eroğlu, 2016). Pelophylax ridibundus (Pallas, 1771) and Bos taurus indicus (Linnaeus, 1758) have symmetric, and between symmetric and asymmetric karyotypes, respectively (Canpolat et al, 2018;Amancio et al, 2019).…”