Banding in Human Chromosomes treated with Trypsin

Wang, H. C.; Fedoroff, S.

doi:10.1038/newbio235052a0

Cited by 463 publications

(120 citation statements)

References 7 publications

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“…All individuals were karyotyped in the microscope and every chromosome arm was identified by G-banding. The trypsin-Giemsa technique of Wang & Fedoroff (1972) was applied. No.…”

Section: Methodsmentioning

confidence: 99%

Reconstruction of the postglacial colonization of Sorex araneus into northern Scandinavia based on karyotype studies, and the subdivision of the Abisko race into three

Fredga¹

2007

Rus.J.Theriol.

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“…All individuals were karyotyped in the microscope and every chromosome arm was identified by G-banding. The trypsin-Giemsa technique of Wang & Fedoroff (1972) was applied. No.…”

Section: Methodsmentioning

confidence: 99%

Reconstruction of the postglacial colonization of Sorex araneus into northern Scandinavia based on karyotype studies, and the subdivision of the Abisko race into three

Fredga¹

2007

Rus.J.Theriol.

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“…For karyotyping, air-dried preparations were banded using pancreatin (Sigma, St. Louis, USA) as reported [18]. The chromosomal constitutions were described according to the recommendations of the ISCN 1991 [19], and calcu lated on the basis of a triploid DNA content, to stress the over-and underrepresentations of (parts of) chromosomes relative to the triploid situation.…”

Section: Slide Preparation and Karyotypingmentioning

confidence: 99%

Comparative genomic hybridization of germ cell tumors of the adult testis: Confirmation of karyotypic findings and identification of a 12p-amplicon

Mostert

Pol

Weghuis

et al. 1996

Cancer Genetics and Cytogenetics

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Comparative genomic hybridization (CGH) was carried out on 15 primary testicular germ cell tumors (TGCT) o f adolescents and adults and two metastatic residual tumors after chemotherapeu tic treatment. The results were compared with karyotypic data obtained form the same tum or specimens after direct harvesting o f metaphases or short-term in vitro culture. .Both techniques revealed that the m ost consistent abnormality in prim ary TGCT is gain o f 12p-sequences. Although In m o st cases over representation o f the complete short arm was observed, CGH revealed a specific amplification of 1 2 p ll.l-p l2 ,l region in two independent primary tumors. In additiont loss of (parts of) chromosome 13 (always involving q31-qter), and gain o f (parts of) chromosome 7 (mostly involving q ll), (parts of) chro mosome 8, and the X chromosome were detected in more than 25% o f the tumors by this latter tech nique. Loss of 6ql5-q21 in both residual tumors analyzed m a y suggest a role for this anomaly in acquired resistance to chemotherapeutic treatm ent Overall, the CGH analyses confirmed gains and losses o f certain chromosomal regions in TGCT as

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“…Schmoll, Hannover, Germany) and two cell lines reported to show SE-like characteristics (i.e., NCCIT [26] For conventional karyotyping, air-dried preparations were banded with pancreatin (Sigma, St. Louis, MO, U.S.A.) as reported previously [27], The chromosome constitution is described according to the International System for Human Cytogenetic Nomenclature [28], except that it is calculated on the basis of a triploid instead of a diploid DNA content because of the consistent peritriploid DNA content of TGCT [4,7].…”

Section: Cell Linesmentioning

confidence: 99%

Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults

Mostert

Pol

Echten

et al. 1996

Cancer Genetics and Cytogenetics

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Overrepresentation o f the short arm of chromosome '12 is frequentty detected in human testicular germ cell tumors of adolescents and adults (TGCT). This overrepresentation m ostly results from the formation of an isochromosome: i{ 12p). Whether the overrepresentation consistently involves I N T R O D U C T I O NHuman testicular germ cell tumors of adolescents and adults (TGCT) can clinically and histologically be grouped Rotterdam; Department of Medical Genetics University Hospital Groningen (J. v E.}, Groningen; and Department of Human Genetics, University Hos pital Nijmegen, (D. O. W., A. G. v K.j into two entities; the seminomas (SE) and nonseminomatous TGCT (NS) [l, 2], All TGCT originate from a common precursor, carcinoma in situ (CIS) [3]. Despite a hypertriploid DNA content for CIS and SE and a hypotriploid DNA content for NS [4-6], they show a striking similarity in From the Laboratory for Experimental Patho-Oncology Dr. Daniel den Hoed Cancer Center; University Hospital Rotterdam

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Banding in Human Chromosomes treated with Trypsin

Cited by 463 publications

References 7 publications

Reconstruction of the postglacial colonization of Sorex araneus into northern Scandinavia based on karyotype studies, and the subdivision of the Abisko race into three

Reconstruction of the postglacial colonization of Sorex araneus into northern Scandinavia based on karyotype studies, and the subdivision of the Abisko race into three

Comparative genomic hybridization of germ cell tumors of the adult testis: Confirmation of karyotypic findings and identification of a 12p-amplicon

Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults

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