The aim of this study was to determine the physiological effects of the audiological test procedure on individuals and the changes in Galvanic Skin Response (GSR). GSR data from 39 volunteers at rest and during the audiological testing were analyzed and the effects of the audiological testing procedure were evaluated. It was observed that the audiological test showed significant differences according to the resting status in terms of mean value, mean power, Root Mean Square (RMS), Kurtosis, and Skewness. The results obtained in the study show that these differences in GSR can be evaluated according to the physiological effect reflections of the emotional changes created on individuals by the audiological test.