Barbaloin inhibits ventricular arrhythmias in rabbits by modulating voltage-gated ion channels

Cao, Zhijun; Tian, Yan; Jiang, Hao; Zhang, Peihua; Liu, Zhi-Pei; Jiang, Wentao; Zeng, Meng-Liu; Zhang, Peipei; Ma, Jing

doi:10.1038/aps.2017.93

Cited by 22 publications

(14 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Barbaloin (Bar), the primary active ingredient in aloe has gained increasing attention (21). A recent study has demonstrated that Bar controls ventricular arrhythmia by regulating voltage-gated ion channels (22). Bar pretreatment inhibits myocardial oxidative stress by activating the AMP-activated protein kinase (AMPK) signaling pathway, thereby alleviating MIRI (23).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

2019

View full text Add to dashboard Cite

Barbaloin (Bar) has a myocardial protective effect, but its mechanism of action is uncertain. The endoplasmic reticulum stress (ERS)-mediated apoptosis pathway serves an important role in the pathogenesis of myocardial ischemia-reperfusion injury (MIRI). Inhibiting ERS may significantly improve the progression of MIRI and serve a role in its prevention. Therefore, based on current knowledge of ERS-mediated cardiomyocyte apoptosis and the cardioprotective effect of Bar, the purpose of the present study was to further evaluate the myocardial protective effect and potential mechanisms of Bar pretreatment in MIRI. The present study established a MIR rat model and randomly divided these rats into four groups. Prior to myocardial ischemia, Bar (20 mg/kg) was administered to rats once daily for 1 week. Myocardial blood serum lactate dehydrogenase and creatine kinase were subsequently measured. A terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assay was used to evaluate the myocardial protective effect of Bar pretreatment on MIRI. To assess whether the ERS signaling pathway was involved in the myocardial protection mechanism of Bar pretreatment, the expression levels of ERS-associated proteins, protein canopy homolog 2 (CNPY2), glucose regulatory protein 78, transcriptional activator 4, C/EBP-homologous protein (CHOP), PKR endoplasmic reticulum kinase (PERK), caspase-12 and caspase-3 were detected by western blot analysis, immunohistochemistry or reverse transcription-quantitative polymerase chain reaction. The results confirmed that Bar pretreatment significantly reduced the damage and the level of apoptosis caused by MIR. Bar pretreatment significantly inhibited the expression of ERS-associated proteins in cardiomyocytes. In addition, the immunohistochemistry results demonstrated that Bar pretreatment significantly inhibited the CNPY2-positive cell apoptosis ratio of cardiomyocytes. Therefore, the results of the current study suggested that CNPY2 is present in cardiomyocytes and participates in the development of MIRI by initiating the PERK-CHOP signaling pathway. Bar pretreatment may attenuate MIRI by inhibiting the CNPY2-PERK apoptotic pathway.

show abstract

Section: Introductionmentioning

confidence: 99%

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

2019

View full text Add to dashboard Cite

show abstract

“…The meanings of all variables were identical to the previous descriptions [14]. As is shown in Figure 2, SH exerted various effects on I Na.P steady state inactivation and steady state activation (Fig.…”

Section: Effects Of Sh On Steady State Activation and Inactivation Ofmentioning

confidence: 85%

“…85-23). Rabbit ventricular cardiomyocytes were isolated enzymatically, as described previously [14]. In brief, medium-sized adult New Zealand white rabbits of either sex were anesthetized with ketamine (Fujian Gutian Pharmaceutical Co., Ltd., Gutian, Fujian, China, 30 mg/kg i.v.)…”

Section: Isolation Of Ventricular Myocytesmentioning

confidence: 99%

See 1 more Smart Citation

Sodium Houttuyfonate Inhibits Voltage-Gated Peak Sodium Current and Anemonia Sulcata Toxin II-Increased Late Sodium Current in Rabbit Ventricular Myocytes

et al. 2018

Self Cite

View full text Add to dashboard Cite

Aim: Sodium houttuyfonate (SH), a chemical compound originating from Houttuynia cordata, has been reported to have anti-inflammatory, antibacterial, and antifungal effects, as well as cardioprotective effects. In this study, we investigated the effects of SH on cardiac electrophysiology, because to the best of our knowledge, this issue has not been previously investigated. Methods: We used the whole-cell patch-clamp technique to explore the effects of SH on peak sodium current (I_Na.P) and late sodium current (I_Na.L) in isolated rabbit ventricular myocytes. To test the drug safety of SH, we also investigated the effect of SH on rapidly activated delayed rectifier potassium current (I_Kr). Results: SH (1, 10, 50, and 100 μmol/L) inhibited I_Na.P in a concentration-dependent manner with an IC₅₀ of 78.89 μmol/L. In addition, SH (100 μmol/L) accelerated the steady state inactivation of I_Na.P. Moreover, 50 and 100 μmol/L SH inhibited Anemonia sulcata toxin II (ATX II)-increased I_Na.L by 30.1 and 57.1%, respectively. However, SH (50 and 100 μmol/L) only slightly affected I_Kr. Conclusions: The inhibitory effects of SH on ATX II-increased I_Na.L may underlie the electrophysiological mechanisms of the cardioprotective effects of SH; SH has the potential to be an effective and safe antiarrhythmic drug.

show abstract

“…Again, barbaloin dose-dependently blocked I Ca-L and in Langendorff mounted hearts significantly inhibited aconitine-induced ventricular arrhythmias. 59 It is reported that Aloe vera may increase the chance of hypokalemia and causing digitalis toxicity and arrhythmia. 23 …”

Section: Plants and Their Ingredients With Antiarhythmic Propertiesmentioning

confidence: 99%

A review on plants and herbal components with antiarrhythmic activities and their interaction with current cardiac drugs

Beik

Joukar

Najafipour

2020

Journal of Traditional and Complementary Medicine

View full text Add to dashboard Cite

This paper aimed to compile information on plants or their compounds which have experimentally shown antiarrhythmic effect and to scrutinize the efficacy and potency of them and their potential interaction with conventional cardiac drugs. Literature searches were accomplished by using numerous electronic databases, and the available knowledge on different parts of herbs and their ingredients with antiarrhythmic effects up to 2019 were identified and collected. The results indicate that 36 herbs or their derivatives can be effective in the treatment of arrhythmias, especially in animal and cellular models. They affect various ionic channels in different action potential phases. The alterations in ionic currents lead to changing in the amplitude and duration of the action potential, effective refractory period, maximum velocity, resting membrane potential, channel trafficking, or intracellular calcium concentration. The agents that prolong action potential duration and effective refractory period such as dauricine and sophocarpine seem to be more beneficial if more comprehensive studies confirm their efficacy and safety. It is noteworthy that the consumption of some herbal agents for cardiovascular (e.g. Hawthorn and Ginseng) or other (e.g. Ginseng and Licorice) therapeutic purposes may boost the pro-arrhythmogenic effect of current cardiovascular drugs such as cardiac glycosides. This study accentuates known plants or their derivatives with anti-arrhythmic effects, potential interaction with other cardiac drugs, and the possible mechanisms involved. It can assist clinicians and scientists in research and therapeutic approaches to the management of cardiac arrhythmias.

show abstract

Barbaloin inhibits ventricular arrhythmias in rabbits by modulating voltage-gated ion channels

Cited by 22 publications

References 45 publications

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

Sodium Houttuyfonate Inhibits Voltage-Gated Peak Sodium Current and Anemonia Sulcata Toxin II-Increased Late Sodium Current in Rabbit Ventricular Myocytes

A review on plants and herbal components with antiarrhythmic activities and their interaction with current cardiac drugs

Contact Info

Product

Resources

About