Barcode DNA Tumbuhan Pangi (Pangium edule R.) Berdasarkan Gen matK

Tuntun angin is one of important floodplain plants in and around Kajuik Lake located in Riau Province, Indonesia. Morphological identification shows that the scientific name of this plant is Elaeocarpus floribundus. The study aimed to confirm the taxonomic status of tuntun angin using matK and nuclear intergenic spacer (ITS) sequences. The methods included fresh leaf DNA isolation, polymerase chain reaction, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.06 programs. The results showed that the matK sequence (519 bp) of tuntun angin had highest similarity to E. floribundus matK sequence that was available in GenBank. It was supported by the high max score (937), low E-value (0.0), high identity value (100%), and high query cover (100%). However, the ITS sequence of tuntun angin did not show similarity to E. floribundus ITS sequence because there was no database of the sequence in GenBank. This study was able to confirm the taxonomic status of tuntun angin as E. floribundus using matK sequence and also showed that morphological and molecular identification techniques were complementary to each other. Moreover, this study enriched the DNA sequence database of E. floribundus in GenBank which will be useful for this species’ molecular identification.How to CiteRoslim, D. I., Khumairoh, S. & Herman, H. (2016). Confirmation of Tuntun Angin (Elaeocarpus floribundus) Taxonomic Status Using matK and ITS Sequences. Biosaintifika: Journal of Biology & Biology Education, 8(3), 393-400.

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“…The result showed that matK sequence is more capable to be a DNA barcode for payus limondok orchid than rbcL sequence. Bangol et al (2014) also…”

Section: Analysis Of Matk and Its Sequences Of Tuntun Anginmentioning

confidence: 97%

Confirmation of Tuntun Angin (Elaeocarpus floribundus) Taxonomic Status Using matK and ITS Sequences

2016

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“…Therefore, DNA barcoding is carried out to be able to identify quickly and accurately. DNA barcoding is a technique used to speed up and simplify the process of identifying organisms using certain gene fragments (Bangola et al, 2014). DNA barcoding techniques can provide a "biological bar code" from short DNA sequences that are standardized to identify a species (Hajibabaei et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

DNA Barcoding and Morphological Characters of Two Trevally Fish species (Caranx Spp.) Collected from Youtefa Bay, Papua, Indonesia

et al. 2023

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Trevally fish (Caranx spp,) or Kuwe fish (local name) are small pelagic fish that are mostly caught in the waters of Youtefa Bay. There are two types of Kuwe fish that are difficult to distinguish due to morphological similarities. These Kuwe fish have no scientific species identification so they have several different local names. Accordingly, it is necessary to identify and find out the proper naming of the two Kuwe fish. DNA barcoding is a technique used to speed up and simplify the process of identifying organisms. Hence, the purpose of this research was to identify the proper scientific name of the two Kuwe species in Youtefa Bay waters, as well as the kinship between these two types by applying a DNA barcoding technique. Nineteen Kuwe fish were sampled at Youtefa Bay, Jayapura, Papua in July 2022. Samples were studied morphologically, then carried directly to the Integrated Laboratory of Diponegoro University for molecular studies. DNA extraction, amplification, and DNA sequencing were carried out to further validate the species’ identification. The results showed that in the initial assessment of the specimens using taxonomic keys, 10 specimens were identified as Caranx sexfasciatus and 9 specimens were identified as Caranx tille. The relationship between length and weight of C. sexfasciatus and C. tille have resulted in b = 2.5754 with R2 = 0.8564 and = 1.7119 with R2 = 0.6705, respectively. All identifications were confirmed as correct based on BLASTn results. The BLAST analysis demonstrated that 10 samples were closely related to C. sexfasciatus (99%-100% in similarity) and 9 samples were similar to C. tille (97%- 99%). The genetic distance between individuals in C. sexfasciatus and C. tille has a value ranging from 0% - 0.2%, and the phylogenetic tree exposed that the two species are included in different clades. This study revealed that there are two species of Kuwe fish (C. sexfasciatus and C. tille) in Youtefa Bay, Papua, Indonesia.

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“…The first flowering time of V. tricolor orchids is relatively long, about five years since seed sowing, therefore, it is difficult to find these orchids in a flowering state in nature [6]. Hence, an alternative identification method is needed other than based on morphological characters, namely DNA barcoding [7].…”

Section: Introductionmentioning

confidence: 99%

“…The matK gene provides high resolution in comparing plant species, but the gene is difficult to amplify. In contrast, the rbcL gene is easy to amplify but has a low resolution so that it is difficult to distinguish species in close relatives [7]. The Internal Transcribed Spacer (ITS) region is a potential barcode originating from the nuclei.…”

Section: Introductionmentioning

confidence: 99%

DNA Barcoding of Vanda tricolor Lindl. Based on matK, rbcL and ITS2 Sequences

Su’udi¹,

Rosida²,

Ravitamala³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Vanda tricolor Lindl. is one of the important ornamental plants favored by many people in the world. However, this plant required many years for vegetative growth before the first flowers emerge. Since the flower is one of the most important organs in morphological identification, an alternative approach through DNA barcoding is one of the best breaking approaches for fast and accurate identification regardless of floral organs availabilty. In this study, three different primer sets (matK, rbcL, ITS2) were used for molecular identification. DNA isolation was carried out using plant genomic isolation kit and was applied as template in PCR reaction. After confirmation using agarose gel electrophoresis and visualized under UV transilluminator, the PCR products were then purified and subjected for sequencing analysis. The sequences length obtained were 408 bp, 317 bp and 461 bp for matK, rbcL, and ITS2, respectively. Further analysis using BLAST and phylogenetic tree construction revealed that all three primer sets are applicable for identification of Vanda orchids. Notably, the ITS2 sequence showed specificity and able to discriminate up to the species level, thus recommended to be used in molecular identification of Vanda orchids.

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Barcode DNA Tumbuhan Pangi (Pangium edule R.) Berdasarkan Gen matK

Cited by 10 publications

References 7 publications

Confirmation of Tuntun Angin (Elaeocarpus floribundus) Taxonomic Status Using matK and ITS Sequences

Confirmation of Tuntun Angin (Elaeocarpus floribundus) Taxonomic Status Using matK and ITS Sequences

DNA Barcoding and Morphological Characters of Two Trevally Fish species (Caranx Spp.) Collected from Youtefa Bay, Papua, Indonesia

DNA Barcoding of Vanda tricolor Lindl. Based on matK, rbcL and ITS2 Sequences

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