Bare surface of gold nanoparticle induces inflammation through unfolding of plasma fibrinogen

Kharazian, Bahar; Lohse, Samuel E.; Ghasemi, Forough; Raoufi, Mohamad; Saei, Amir Ata; Hashemi, Fatemeh; Farvadi, Fakhrossadat; Alimohamadi, Reza; Jalali, Seyed Amir; Shokrgozar, Mohammad Ali; Hadipour, Nasser L.; Ejtehadi, Mohammad Reza; Mahmoudi, Morteza

doi:10.1038/s41598-018-30915-7

Cited by 45 publications

(32 citation statements)

References 39 publications

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“…Thus, this suggests that the cleavage site would be readily available to thrombin and would support our spectrometric data highlighting a conformational change in fibrinogen, rather than its aggregation. This is also supported by literature demonstrating that nanoparticles indeed can affect fibrinogen conformation, [45][46][47][48][49][50] including iron oxide nanoparticles. [31] Accordingly, Zhang et al reported that UV-vis spectroscopy of bovine fibrinogen incubated with 10 nm γ-Fe 2 O 3 indicated a decrease in absorbance intensity in the peaks at 218 and 275 nm when compared to fibrinogen.…”

Section: Discussionsupporting

confidence: 81%

Anti‐Platelet Effect Induced by Iron Oxide Nanoparticles: Correlation with Conformational Change in Fibrinogen

Kottana

Maurizi

Schnoor

et al. 2020

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Iron oxide nanoparticles are developed for various biomedical applications, however, there is limited understanding regarding their effects and toxicity on blood components. The particles traveling in circulation inevitably interact with blood cells and plasma proteins and may interfere with hemostasis. Specifically, this study focuses on the influence of superparamagnetic iron oxide nanoparticles (SPIONs) coated with a biocompatible polymer, polyvinyl alcohol (PVA), on platelet function. Here, engineered SPIONs that are functionalized with various PVA coatings to provide these particles with different surface charges and polymer packing are described. These formulations are assessed for any interference with human platelet functions and coagulation, ex vivo. Positively charged SPIONs induce a significant change in platelet GPIIb‐IIIa conformation, indicative of platelet activation at the dose of 500 µg mL−1. Remarkably, engineered PVA(polyvinyl alcohol)‐SPIONs all display a robust dose‐dependent anti‐platelet effect on platelet aggregation, regardless of the PVA charge and molecular weight. After assessing hypotheses involving SPION‐induced steric hindrance in platelet–platelet bridging, as well as protein corona involvement in the antiplatelet effect, the study concludes that the presence of PVA‐SPIONs induces fibrinogen conformational change, which correlates with the observed dose‐dependent anti‐platelet effect.

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Section: Discussionsupporting

confidence: 81%

Anti‐Platelet Effect Induced by Iron Oxide Nanoparticles: Correlation with Conformational Change in Fibrinogen

Kottana

Maurizi

Schnoor

et al. 2020

Small

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“…Citrate‐stabilized GNPs do not activate inflammatory pathway in THP‐1 cells, and instead, negatively regulate the inflammatory process, by selectively targeting IL‐1‐dependent pathway (Kharazian et al, ). GNPs downregulate the IL‐1‐induced responses in vivo.…”

Section: Discussionmentioning

confidence: 99%

Effects of treatment with gold nanoparticles in a model of acute pulmonary inflammation induced by lipopolysaccharide

Haupenthal

Mendes

Silveira

et al. 2019

J Biomedical Materials Res

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The bacterial lipopolysaccharide (LPS) is a highly toxic molecule derived from the outer membrane of gram-negative bacteria. LPS endotoxin affects the lungs and is used as a model of acute pulmonary inflammation affecting the cellular morphology of the organ. Previously, gold nanoparticles (GNPs) have been shown to demonstrate anti-inflammatory and antioxidative activity in muscle and epithelial injury models.The objective of this study was to investigate the effect of the intraperitoneal treatment using GNPs on the inflammatory response and pulmonary oxidative stress induced by LPS. Wistar rats were divided into four groups (N = 10): Sham; Sham + GNPs 2.5 mg/kg; LPS; and LPS + GNPs 2.5 mg/kg. Treatment with LPS upregulated the levels of markers of cellular and hepatic damage (CK, LDH, AST, and alanine aminotransferase); however, the group treated with only GNPs exhibited no toxicity.Treatment with GNPs reversed LPS-induced changes with respect to total peritoneal leukocyte count and the pulmonary levels of pro-inflammatory cytokines (IFN-γ and IL-6). Histological analysis revealed that treatment with GNPs reversed the increase in alveolar septum thickness due to LPS-induced fibrosis. In addition, treatment with GNPs decreased production of oxidants (nitrite and DCFH), reduced oxidative damage (carbonyl and sulfhydryl), and downregulated activities of superoxide dismutase and catalase. Treatment with GNPs did not showed toxicity; however, it exhibited anti-inflammatory and antioxidative activity that reversed morphological alterations induced by LPS. K E Y W O R D S gold nanoparticles, inflammation, lipopolysaccharide, lung, oxidative stress

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“…However, due to the large surface of curvature, despite surface coating with moieties like PEG, ultrasmall GNPs can have gaps that can be filled with blood proteins such as fibrinogen. As a result, smaller GNPs can contribute to an inflammatory response, due to their interactions with these proteins, highlighting the necessity for proper functionalization [91]. Another factor that impacts biodistribution is the surface charge, which can be controlled by various surface conjugations, such as with PEG [92,93].…”

Section: Intracellular Fate Of Gold Nanoparticles Based On Their Physmentioning

confidence: 99%

Advances in Gold Nanoparticle-Based Combined Cancer Therapy

Bromma

Chithrani

2020

Nanomaterials

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According to the global cancer observatory (GLOBOCAN), there are approximately 18 million new cancer cases per year worldwide. Cancer therapies are largely limited to surgery, radiotherapy, and chemotherapy. In radiotherapy and chemotherapy, the maximum tolerated dose is presently being used to treat cancer patients. The integrated development of innovative nanoparticle (NP) based approaches will be a key to address one of the main issues in both radiotherapy and chemotherapy: normal tissue toxicity. Among other inorganic NP systems, gold nanoparticle (GNP) based systems offer the means to further improve chemotherapy through controlled delivery of chemotherapeutics, while local radiotherapy dose can be enhanced by targeting the GNPs to the tumor. There have been over 20 nanotechnology-based therapeutic products approved for clinical use in the past two decades. Hence, the goal of this review is to understand what we have achieved so far and what else we can do to accelerate clinical use of GNP-based therapeutic platforms to minimize normal tissue toxicity while increasing the efficacy of the treatment. Nanomedicine will revolutionize future cancer treatment options and our ultimate goal should be to develop treatments that have minimum side effects, for improving the quality of life of all cancer patients.

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Bare surface of gold nanoparticle induces inflammation through unfolding of plasma fibrinogen

Cited by 45 publications

References 39 publications

Anti‐Platelet Effect Induced by Iron Oxide Nanoparticles: Correlation with Conformational Change in Fibrinogen

Anti‐Platelet Effect Induced by Iron Oxide Nanoparticles: Correlation with Conformational Change in Fibrinogen

Effects of treatment with gold nanoparticles in a model of acute pulmonary inflammation induced by lipopolysaccharide

Advances in Gold Nanoparticle-Based Combined Cancer Therapy

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