Barley

Ganeshan, S.; Chibbar, Ravindra N.; Dahleen, Lynn S.; Tranberg, Janice; Lemaux, Peggy G.

doi:10.1002/9781405181099.k0104

Cited by 3 publications

(1 citation statement)

References 189 publications

(178 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rapid progress in tissue culture and transformation technologies has allowed successful production of transgenic plants in most cereal crops (Lemaux et al 1999 ; Vasil 2007 ; Ganeshan et al 2008 ). For practical application of these technologies, such as trait improvement and gene flow studies, it is essential that introduced genes of interest be stably expressed and transmitted intact during generation advance and following outcrossing.…”

Section: Introductionmentioning

confidence: 99%

Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants

Choi

Lemaux

et al. 2009

Plant Cell Rep

Self Cite

View full text Add to dashboard Cite

To study stability and inheritance of two different transgenes in barley, we crossed a homozygous T 8 plant, having uidA (or gus) driven by the barley endosperm-specific B 1 -hordein promoter (localized in the near centromeric region of chromosome 7H) with a second homozygous T 4 plant, having sgfp(S65T) driven by the barley endosperm-specific D-hordein promoter (localized on the subtelomeric region of chromosome 2H). Both lines stably expressed the two transgenes in the generations prior to the cross. Three independently crossed F 1 progeny were analyzed by PCR for both uidA and sgfp(S65T) in each plant and functional expression of GUS and GFP in F 2 seeds followed a 3:1 Mendelian segregation ratio and transgenes were localized by FISH to the same location as in the parental plants. FISH was used to screen F 2 plants for homozygosity of both transgenes; four homozygous plants were identified from the two crossed lines tested. FISH results showing presence of transgenes were consistent with segregation ratios of expression of both transgenes, indicating that the two transgenes were expressed without transgene silencing in homozygous progeny advanced to the F 3 and F 4 generations. Thus, even after crossing independently transformed, homozygous parental plants containing a single, stably expressed transgene, progeny were obtained that continued to express multiple transgenes through generation advance. Such stability of transgenes, following outcrossing, is an important attribute for trait modification and for gene flow studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants

Choi

Lemaux

et al. 2009

Plant Cell Rep

Self Cite

View full text Add to dashboard Cite

show abstract

Application of Molecular Genetics and Transformation to Barley Improvement

Thomas¹,

Hayes

Dahleen³

2010

Barley

View full text Add to dashboard Cite

In Vitro Assessment of Salinity Stress Impact on Early Growth in Ten Certified Palestinian Barley Cultivars (Hordeum vulgare L.) Potentially Suitable for Cultivation on Former Quarry Substrates

Al-Tardeh

Alqam

Kuhn

et al. 2023

Water

View full text Add to dashboard Cite

Salinity is a major constraint for crop health and productivity, particularly on arid, semiarid, and otherwise marginal soils, such as quarry residue. Quarries are a main pillar of national income in Palestine but have a long-lasting toll on the environment. We examined barley (Hordeum vulgare L.), another pillar of the Palestinian economy and one of the most important crops in the world, in this regard for its tolerance to salinity stress. This study is the first to evaluate the impact of salinity (50, 85, 120, and 175 mM NaCl) on seed germination, early growth stage, and morpho-anatomy on ten pre-selected certified Palestinian barley cultivars (Baladi, Improved Baladi, Rihan, ICARDA 1, ICARDA 15, ACSAD 68, ACSAD 176, ACSAD 1417, ACSAD 1732, and ACSAD 1744) to assess their potential for a successful growth start under adverse saline conditions. In addition, soil samples from quarries in Hebron governorate were randomly selected and tested for salinity level, electrical conductivity, and total of soluble salts for a first rough overview of options for applying our results, since local data are often scarce or outdated. The examined soil samples reached electrical conductivity (EC) ranges of 1.81 × 10−4–9.071 × 10−4 dS m−1, which are below the normal EC (11–57 × 10−4 dS m−1). This result may contraindicate the hypothesis that quarry lands always suffer from salinity stress. Cultivars such as ACSAD 68 and Icarda 15 proved very sensitive to higher salinity stress with high G50 (time point when 50% of seeds have germinated) at 4.4 d, with 120 mM NaCl (ACSAD 68) or incalculable amounts (Icarda 15) and just 50 and 20% total germination, respectively. Concentrations of 175 mM NaCl were found in ACSAD 176 and Improved Baladi (no G50, 37 and 30% germination, respectively). Some cultivars showed a moderate to high resilience to salinity, such as ICARDA I, ACSAD 1417, and ACSAD 1744, which reached > 80% seed germination at 120 mM NaCl and >60% at 175 mM NaCl, and G50 within 1.5–2.2 days; the most resilient was ACSAD 1732 with G50 < 2 days and germination still >80% at 175 mM NaCl. This is strongly supported by the monitored growth parameters. In conclusion, ACSAD1732 and Icarda 1 cultivars are highly recommended for cultivation in areas of low precipitation and high salt accumulation. In addition, the land and/or soil of quarries, their landfills, and nearby areas in Palestine may be fit for barley cultivation with recommended cultivars regarding salinity stress.

show abstract

Barley

Cited by 3 publications

References 189 publications

Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants

Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants

Application of Molecular Genetics and Transformation to Barley Improvement

In Vitro Assessment of Salinity Stress Impact on Early Growth in Ten Certified Palestinian Barley Cultivars (Hordeum vulgare L.) Potentially Suitable for Cultivation on Former Quarry Substrates

Contact Info

Product

Resources

About