Barley Dhn13 encodes a KS-type dehydrin with constitutive and stress responsive expression

Rodriguez, Edmundo; Svensson, Jan T.; Malatrasi, Marina; Choi, Dong-Woog; Close, Timothy J.

doi:10.1007/s00122-004-1877-4

Cited by 74 publications

(46 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Microarray data showed a considerable increase of Dhn13 transcripts when plants were subjected to chilling and freezing temperatures. Rodriguez et al (2005), supporting our results, also found that there were somewhat elevated transcript accumulation of the Dhn13 gene in barley under drought stress. Compared with Dhn6 and Dhn11, lower accumulation of Dhn13 during dehydration may be due to less efficient signaling pathways and transcriptional activators.…”

Section: Differential Expression Of Dhn Genessupporting

confidence: 91%

“…The Dhn gene family encodes for several proteins that are typically accumulated during the maturation-drying phase of seed development, in seedlings, or more mature plants in response to environmental stresses (Shinozaki and YamaguchiShinozaki 1997;Zhu et al 2000). In the barley genome, recent investigations into the dehydrin multigene family have identified 13 Dhn genes, which encode four subclasses of DHNs, Y n SK n , SK n , K n and KS, based on permutations in the arrangement of characterized domains [reviewed in Campbell and Close (1997), Werner-Fraczek and Close (1998), Choi et al (1999Choi et al ( , 2000, Svensson et al (2002), Rodriguez et al (2005)]. Transcriptome analyses based on microarrays have provided powerful tools for the discovery of stressresponsive genes, not only in Arabidopsis, but also in various crop plants and tree species (Shinozaki and Yamaguchi-Shinozaki 2007).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress

Qian¹,

Liu²,

Ao³

et al. 2008

Can. J. Plant Sci.

View full text Add to dashboard Cite

M. 2008. Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress. Can. J. Plant Sci. 88: 899Á906. Dehydrins (DHN) are water-soluble lipid-associating proteins that accumulate during low-temperature and water-stress conditions, and are thought to play a protective role in freezing and drought tolerance in plants. The synthesis of the dehydrins, representing a link between environment and nuclear activity through dehydrin genes (Dhn) expression, was known to be a common response to drought in plants. The association of mRNA accumulations of Dhn genes (Dhn6, Dhn11 and Dhn13) with phenotypically diverse drought-tolerant genotypes was investigated in hull-less barley (Hordeum vulgare subsp. vulgare). Notably, differences between genotypes were observed, mainly in Dhn6 and Dhn13 gene, depending on the duration of the dehydration stress. Compared with drought-sensitive genotypes, the relative expression levels of Dhn6 gene were significantly higher in drought-tolerant lines subjected to 8 h water deficit. By control with continuative ascending accumulations in sensitive plants during drought, the highest transcript level of Dhn13 was observed in tolerant genotypes after 8 h of dehydration, and tended to descend the same levels as sensitive lines subjected to 12 h of dehydration. Based on decreased accumulations of Dhn11 during drought stress, it was concluded that Dhn11 was not up-regulated by water stress between genotypes. The observed differences in the present study may indicate an association of physiological response to water stress with differential accumulations of Dhn genes between drought-contrasting genotypes. The authors suggest different DHNs may play variable functional roles in structural protection in plants subjected to progressive water stress, associated with drought-contrasting genotypes.Key words: Dhydrin genes, quantitative reverse transcription polymerase chain reaction, water deficit, Tibetan hull-less barley Qian, G., Liu, Y., Ao, D., Yang, F. et Yu, M. 2008. Variation de l'expression des ge`nes de la de´hydrine dans une orge nue (Hordeum vulgare ssp. vulgare) selon la dure´e de la de´shydratation. Can. J. Plant Sci. 88: 899Á906. La de´hydrine (DHN) est une prote´ine lipophile hydrosoluble qui s'accumule quand la tempe´rature descend et qu'il y a stress hydrique. On pense qu'elle prote`ge la plante contre le gel et lui permet de tole´rer la se´cheresse. La synthe`se des de´hydrines, qui repre´sente un lien entre l'environnement et l'activite´du noyau par le biais des ge`nes qui la codent (Dhn), est une re´action commune a`la se´cheresse chez les ve´ge´taux. Les auteurs ont e´tudie´la relation entre l'accumulation de l'ARNm des ge`nes Dhn (Dhn6, Dhn11 et Dhn13) et des varie´te´s de phe´notype variable tole´rant la se´cheresse chez l'orge nue (Hordeum vulgare subsp. vulgare). Ils ont ainsi observe´des variations entre les ge´notypes, principalement au niveau des ge`nes Dhn6 et Dhn13, selon la dure´e du stress engend...

show abstract

Section: Differential Expression Of Dhn Genessupporting

confidence: 91%

mentioning

confidence: 99%

Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress

Qian¹,

Liu²,

Ao³

et al. 2008

Can. J. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Changes in membrane composition , organic acids (Timpa et al, 1986), accumulation of osmolytes (Hitz et al, 1982;Timpa et al, 1986), and synthesis of stress protection proteins (hsps, Late Embryogenesis Abundant proteins, etc. ; Burke et al, 1985b;Rodriguez et al, 2005) all contribute to the rate of tissue decline in the high respiratory environment used in the bioassay. Further study is needed to determine the overall contribution of individual metabolic pathways to the observed water-deficit stress responses of this bioassay.…”

Section: Impact Of Sampling Time On the Stress Test Determinationmentioning

confidence: 99%

Evaluation of Source Leaf Responses to Water-Deficit Stresses in Cotton Using a Novel Stress Bioassay

Burke

2006

Plant Physiology

View full text Add to dashboard Cite

Water-deficit stresses preferentially reduce shoot growth, thereby disrupting the flow of carbohydrates from source leaves to the developing sinks. Here, we use a novel stress bioassay to dissect responses of field and greenhouse-grown cotton (Gossypium hirsutum) source leaves to water-deficit stresses. Fifth main stem leaf samples were harvested at sunrise and subjected to a prolonged elevated respiratory demand in the dark. Sucrose levels are lower in nonstressed cotton at sunrise compared to waterdeficit stressed cotton, potentially predisposing the nonstressed tissue to succumb more rapidly. Tissue death was determined initially using the cell viability stain 2,3,5-triphenyltetrazolium chloride, but was determined in subsequent experiments by monitoring the decline in chlorophyll fluorescence yield. Fluorescence yield measurements were obtained within minutes of harvesting and individual samples were monitored over the time course of the treatment. Analyses of the time course and magnitude of chlorophyll fluorescence yield decline in samples from irrigated and dryland plots permitted the detection of stress responses within 24 h of the cessation of irrigation. The rate of fluorescence yield decline during the elevated respiratory demand treatment slowed as the water-deficit stress increased. Upon irrigation, the source leaves of the water-stressed plants recovered to prestress values within 4 d. Well-watered cotton overexpressing heat shock protein 101 had identical rates of fluorescence yield decline as nontransgenic cotton. These results suggest that the delayed decline in fluorescence yield of water-stressed tissue exposed to prolonged elevated respiratory demand can be used as a sensitive indicator of water-deficit stress responses.

show abstract

“…Some DHNs are expressed constitutively during normal growth (Nylander et al, 2001;Rorat et al, 2004Rorat et al, , 2006Rodriguez et al, 2005). DHNs exist in a wide range of photosynthetic organisms, including angiosperms, gymnosperms, algae, and mosses (Svensson et al, 2002).…”

mentioning

confidence: 99%

The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural Changes

et al. 2009

View full text Add to dashboard Cite

Dehydrins (DHNs; late embryogenesis abundant D11 family) are a family of intrinsically unstructured plant proteins that accumulate in the late stages of seed development and in vegetative tissues subjected to water deficit, salinity, low temperature, or abscisic acid treatment. We demonstrated previously that maize (Zea mays) DHNs bind preferentially to anionic phospholipid vesicles; this binding is accompanied by an increase in α-helicity of the protein, and adoption of α-helicity can be induced by sodium dodecyl sulfate. All DHNs contain at least one “K-segment,” a lysine-rich 15-amino acid consensus sequence. The K-segment is predicted to form a class A2 amphipathic α-helix, a structural element known to interact with membranes and proteins. Here, three K-segment deletion proteins of maize DHN1 were produced. Lipid vesicle-binding assays revealed that the K-segment is required for binding to anionic phospholipid vesicles, and adoption of α-helicity of the K-segment accounts for most of the conformational change of DHNs upon binding to anionic phospholipid vesicles or sodium dodecyl sulfate. The adoption of structure may help stabilize cellular components, including membranes, under stress conditions.

show abstract

Barley Dhn13 encodes a KS-type dehydrin with constitutive and stress responsive expression

Cited by 74 publications

References 34 publications

Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress

Differential expression of dehydrin genes in hull-less barley (Hordeum vulgare ssp. vulgare) depending on duration of dehydration stress

Evaluation of Source Leaf Responses to Water-Deficit Stresses in Cotton Using a Novel Stress Bioassay

The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural Changes

Contact Info

Product

Resources

About