Barley mutants with enhanced level of resistance to Swedish isolates of <i>Bipolaris sorokiniana</i>, casual agent of spot blotch

Persson, Mattias; Rasmussen, Morten; Falk, Anders; Dixelius, Christina

doi:10.1111/j.1439-0523.2008.01527.x

Cited by 12 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our attempts to further characterize the bst1 mutant, it became clear that B. sorokiniana has a very slow growth on this genotype compared with the wild‐type. This observation is in accordance with a more extensive study in which lesion formation on leaves caused by a larger set of fungal isolates was compared on 22 different barley accessions (Persson et al. , 2008).…”

Section: Discussionsupporting

confidence: 91%

“…The bst1 mutant was identified in a population composed of 5000 M 2 sown spikes derived from fast neutron‐irradiated seeds of the wild‐type cultivar Bowman (Persson et al. , 2008).…”

Section: Resultsmentioning

confidence: 99%

“…The Bipolaris sorokiniana tolerant 1 ( bst1 ) barley mutant has previously been identified as a genetic source for resistance against fungal spot blotch disease (Persson et al. , 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The Bipolaris sorokiniana tolerant 1 (bst1) barley mutant has previously been identified as a genetic source for resistance against fungal spot blotch disease (Persson et al, 2008). bst1 exhibits conspicuous black or brown spots that appear on all above-ground parts of the plant, including the bristles, with the lesion phenotype most prominent on the leaves.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Studies on the mechanism of resistance to Bipolaris sorokiniana in the barley lesion mimic mutant bst1

Persson¹,

Falk

Dixelius

2009

Molecular Plant Pathology

View full text Add to dashboard Cite

SUMMARY The Bipolaris sorokiniana tolerant 1 (bst1) barley mutant is derived from fast neutron-irradiated seeds of wild-type Bowman(Rph3). The induced mutation was genetically localized to a position on chromosome 5HL distal to the centromere using amplified fragment length polymorphism markers. In addition, the defence responses and related gene expression in the bst1 mutant after fungal challenge were compared with those occurring in wild-type plants. Hydrogen peroxide generation, determined by 3,3-diaminobenzidine staining, revealed a clearly reduced level of bst1, compared with the wild-type, during the entire experimental time: 8-120 h post-inoculation (hpi). At 48 hpi, the wild-type samples displayed twice as much fungal mass and three times greater H(2)O(2) production than bst1. At the same time, staining of B. sorokiniana showed less fungal growth in the spontaneous lesions of bst1 compared with the wild-type. Monitoring of defence-related genes at 48 hpi demonstrated strong expression of PR-1a, PR-2, PR-5 and PR-10 in bst1. A gene coding for a unique oxidoreductase enzyme, designated as HCP1, was expressed at much higher levels in inoculated leaves of the bst1 mutant than in those of the wild-type plant. Taken together, the results suggest that the defence to B. sorokiniana largely relies on salicylic acid-responsive pathogenesis-related (PR) genes, as well as selected reactive oxygen species and unknown HCP1-associated factors.

show abstract

Section: Discussionsupporting

confidence: 91%

“…The bst1 mutant was identified in a population composed of 5000 M 2 sown spikes derived from fast neutron‐irradiated seeds of the wild‐type cultivar Bowman (Persson et al. , 2008).…”

Section: Resultsmentioning

confidence: 99%

“…The Bipolaris sorokiniana tolerant 1 ( bst1 ) barley mutant has previously been identified as a genetic source for resistance against fungal spot blotch disease (Persson et al. , 2008).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Studies on the mechanism of resistance to Bipolaris sorokiniana in the barley lesion mimic mutant bst1

Persson¹,

Falk

Dixelius

2009

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Mutant necrotic leaf spot 8.3550 ( nec8 . 3550 ) has previously been demonstrated to confer an increased level of resistance to the barley spot blotch fungus (Persson et al ., ). The objective of the present work was to test the level of resistance of this mutant to other important fungal pathogens of barley.…”

Section: Methodsmentioning

confidence: 99%

Barley lesion mimics, supersusceptible or highly resistant to leaf rust and net blotch

Wright

Azarang²,

Falk

2012

Plant Pathology

View full text Add to dashboard Cite

Lesion mimic mutants of plants have the feature of spontaneously displaying necrotic spots or bands on their leaves. Lesion mimics have often displayed enhanced resistance to biotrophic pathogens whilst showing increased susceptibility to necrotrophs. This paper identifies three novel, non-allelic mutants of barley (Hordeum vulgare), which spontaneously form necrotic leaf lesions: Necrotic leaf spot 9.3091 (nec9.3091), Mottled leaf 8.1661 (mtt8.1661) and Mottled leaf 9.2721 (mtt9.2721). The Necrotic leaf spot 8.3550 mutant (nec8.3550), formerly known as bst1, was included in the study because it is a lesion mimic mutant belonging to the same original pool. The reactions of the mutants to the biotroph Puccinia hordei and the necrotroph Pyrenophora teres f. sp. teres were investigated. Mutants nec8.3550 and mtt8.1661 were more resistant than the parental Bowman near-isogenic line with the Rph3.c gene (Bowman Rph3.c, NGB 22452) to leaf rust, caused by P. hordei. Mutants nec8.3550, mtt8.1661 and mtt9.2721 were more susceptible than Bowman Rph3.c to net blotch, caused by P. teres f. sp. teres. Autofluorescence was detected in leaf tissues of all mutants. Based on the high expression of the PR1 and Hv-HIR genes, combined with the low susceptibility to P. hordei, nec8.3550 appears to have entered a state of systemic acquired resistance, which is quite distinct from the resistance expressed in mtt8.1661. The latter mutant has low or no expression of PR1 and Hv-HIR genes, yet it is highly resistant to rust. It is also extremely susceptible to net blotch. These mutants can serve as genetic sources of novel disease resistance for barley improvement.

show abstract

Characterization and mapping of a spotted-leaf genotype, spl Y181 that confers blast susceptibility in rice

et al. 2014

View full text Add to dashboard Cite

Barley mutants with enhanced level of resistance to Swedish isolates of Bipolaris sorokiniana, casual agent of spot blotch

Abstract: Spot blotch, caused by Bipolaris sorokiniana,

Cited by 12 publications

References 26 publications

Studies on the mechanism of resistance to Bipolaris sorokiniana in the barley lesion mimic mutant bst1

Studies on the mechanism of resistance to Bipolaris sorokiniana in the barley lesion mimic mutant bst1

Barley lesion mimics, supersusceptible or highly resistant to leaf rust and net blotch

Characterization and mapping of a spotted-leaf genotype, spl Y181 that confers blast susceptibility in rice

Contact Info

Product

Resources

About