Barley seed sensitivity to water stress at germination stage

Abstract

Barley seed sensitivity to water and anoxia was tested. Standard germination, mean time of germination (MTG), germination in sand wetted by water to 100% water capacity (anoxia) or by hydrogen peroxide (wet conditions without anoxia), germination in 0.75% hydrogen peroxide and laboratory emergence (15 and 20°C) were evaluated. Barley seed responds sensitively to stress conditions during germination. Significant germination decrease was found in abundance of water. Percentage of reduction depends on the variety and on the year of seed production. Extreme values of water sensitivity are in interval 490%. At wetted sand by 0.75%, solution of H 2 O 2 the germination was significantly less reduced. That means that barley seed is very sensitive to oxygen deficiency above all and is less injured by quick imbibition. Heterogeneity in seed vigour was demonstrated in laboratory emergence tests. Quick test of germination in 0.75% hydrogen peroxide deserves attention for its high correlation coefficient with the seed laboratory emergence. The results significantly demonstrate a higher sensitivity of deteriorated seed to germination in abiotic stresses conditions. Variability in speed of germination is increasing, which unfavourably extends the mean time of germination.

Estimation of Barley Seed Vigour with Respect to Variety and Provenance Effects

“…Although MODARRESI and VAN DAMME (2003) suggested the treatment period of 48 h as optimal for wheat, it showed to be too severe for barley, deterioration for 48 h decreased the germination rate to 4.2% at the moisture content of 22%; and almost all the seeds were killed at the moisture content of 24%. The high sensitivity of barley seeds to environmental stresses was described by HOSNEDL and HONSOVÁ (2002). Further investigations will be done on a larger number of samples to test this method.…”

“…The deterioration technique is in principle similar to the AA test with the exception that the seed moisture is artificially raised to the same level for all samples prior to the period of deterioration at high temperatures. Using AA tests on barley was reported by HOSNEDL and HONSOVÁ (2002). Although we have not found any references to the CD test for barley seeds, the applications of this test to other grain crops were reported, e.g.…”

Testování vitality semen polních plodin

“…Kromě vysokého procenta klíčivosti osiva je pro pěstitele důležitá uniformita a rychlost klíčení semen, dávající předpoklad vitálního klíčence, rovnoměrně vzcházejícího a brzy zapojeného porostu bez mezer s vysokým potenciálem konkurenceschopnosti vůči plevelům. K tomu může přispět vyšší odolnost vitálních semen vůči půdní hypoxii, než je tomu u semen s nízkou vitalitou, jak uvádí Hosnedl a Honsová (2002). Rostliny ze semen s dobrou vitalitou potom rychleji zastíní povrch půdy a tím sníží ztráty vody evaporací na počátku vegetace (Spielmeyer et al, 2007).…”

Response of hydrogenase enzymes (ALDH and ADH) in <i>Trapa natans</i> under physiological stress

“…Oxygen requirement in the seeds of higher plants is well understood for the natural growth. Although there are many examples of stress responsive growth and germination of seeds under have already been noticed by different group of researchers [1][2][3][4]. Most plants can tolerate anoxia for only short periods of time before irreparable damage occurs [5] but in the case of Trapa natans, as it mainly found in water, these conditions are more frequent and most obvious.…”