Baroinactivation of Staphylococcus epidermidis - mathematical model and its verification using human and cow milk

Schlemmerova, Ljuba; Houška, Milan; Špelina, Vladimír; Strohalm, Jan; Landfeld, A.; Šmuhařová, H.; I, Nĕmcová; Kýhos, Karel; Průchová, Jiřina; Novotná, Pavla; Měřička, Pavel

doi:10.17221/1051-cjfs

Cited by 2 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Changing technology to the innovative pasteurization procedures recently described [ 20 , 22 ] might improve discard rates, it would require a long period of experimental verification. High-pressure inactivation, which Demazeau described as being effective against spores [ 23 ], is regarded by us to be a promising method for the future; as our group also found the technique effective on bacteria present in human and cow milk in the past [ 33 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience

Jandová

Měřička

Fišerová

et al. 2021

Foods

View full text Add to dashboard Cite

A systematic study, performed from 2017–2020 looked at the rate of positive post-pasteurization B. cereus findings, the quantity of B. cereus in pasteurized banked human milk (PBM), and the rate of B. cereus toxicogenic isolates from PBM. During the study period, 6815.71 L (30,943 tested bottles) of PBM were tested, with an average amount per year of 1703.93 L (7736 tested bottles). The PBM discard rate per year due to bacterial contamination varied between 8.7–10.0% and contamination with B. cereus was the most frequent reason. The total number of B. cereus positive tests was 2739 and the proportion of its positivity from all positive tests was between 56.7–66.6%. The prevalence of B. cereus positive tests rose significantly in the summer months. The production of enterotoxin was found in 3 of the 20 tested samples (15.0%). The B. cereus CFU-quantities in the PBM were below 10 CFU/mL in 80% of cases (16 of 20 samples tested). The quantitative data can be used in the risk assessment of cold storage of PBM at temperatures above zero and manipulation of PBM prior to its administration.

show abstract

Section: Discussionmentioning

confidence: 99%

“…These characteristics of B. cereus must be considered during risk assessment of cold storage of PBM as well as storage and use of PMB on the pediatric wards. There are growth models available for many microbes surviving pasteurization [ 25 , 35 , 37 ], which are suitable tools for properly assessing this risk.…”

Section: Discussionmentioning

confidence: 99%

Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience

Jandová

Měřička

Fišerová

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…The methods of predictive microbiology are generally regarded to be a standard tool for assessing the risk of food, the properties of which are changing over time because of the presence of viable bacteria. We used this method in the past for predicting the growth of several microbes, including B. cereus, during the processing of human milk [ 35 ] and for evaluating thermo- and baro-inactivation of E. faecium and St. epidermidis in human and cow milk [ 36 , 37 ]. This paper applies this method to simulate manipulations with thawed PBM in neonatal hospital wards.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Risk Assessment of Bacillus cereus Growth during the Warming of Thawed Pasteurized Human Banked Milk Using a Predictive Mathematical Model

Jandová

Měřička

Fišerová

et al. 2022

Foods

View full text Add to dashboard Cite

Bacillus cereus is relatively resistant to pasteurization. We assessed the risk of B. cereus growth during warming and subsequent storage of pasteurized banked milk (PBM) in the warmed state using a predictive mathematical model. Holder pasteurization followed by storage below −18 °C was used. Temperature maps, water activity values, and B. cereus growth in artificially inoculated PBM were obtained during a simulation of manipulation of PBM after its release from a Human Milk Bank. As a real risk level, we chose a B. cereus concentration of 100 CFU/mL; the risk was assessed for three cases: 1. For an immediate post-pasteurization B. cereus concentration below 1 CFU/mL (level of detection); 2. For a B. cereus concentration of 10 CFU/mL, which is allowed in some countries; 3. For a B. cereus concentration of 50 CFU/mL, which is approved for milk formulas. In the first and second cases, no risk was detected after 1 h of storage in the warmed state, while after 2 h of storage, B. cereus concentrations of 102 CFU/mL were occasionally encountered. In the third case, exceeding the B. cereus concentration of 102 CFU/mL could be regularly expected after 2 h of storage. Based on these results, we recommend that post-pasteurization bacteriological analysis be performed as recommended by the European Milk Bank Association (EMBA) and using warmed PBM within 1 h after warming (no exceptions).

show abstract

Baroinactivation of Staphylococcus epidermidis - mathematical model and its verification using human and cow milk

Abstract: Schlemmerová L., Houška M., Špelina V., Strohalm J., Landfeld A., Šmuhařová H., Němcová I., Kýhos K., Průchová J., Novotná P., Měřička P.

Cited by 2 publications

References 6 publications

Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience

Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience

Quantitative Risk Assessment of Bacillus cereus Growth during the Warming of Thawed Pasteurized Human Banked Milk Using a Predictive Mathematical Model

Contact Info

Product

Resources

About