Barrier properties of ex vivo porcine intestinal mucus are highly independent of isolation and storage conditions

Characterizing interregional differences in the rheological properties and composition of rat small intestinal mucus

“…Similar to the rheological ow curves, the G′ of rat intestinal mucus (G′ at ~ 10 3 -10 5 Pa; Figure 3A) was generally higher than what has been reported in pigs (G′ at ~ 10 1 -10 2 Pa [6, [22][23][24]) and dogs (G′ ~ 10 1 -10 2 Pa [8]). While the tan δ of porcine duodenal mucus was lower than the rat duodenal mucus (0.15-0.16 [11,24] vs. 0.3), studies have observed an increase in tan δ to 0.3-0.6 for porcine small intestinal mucus [10,23,24], which is similar to what was observed in the current study (Figure 3C).…”

Development and validation of a porcine artificial colonic mucus model reflecting the properties of native colonic mucus in pigs

“…In conclusion, the storage conditions did not alter the physicochemical interaction characteristics or steric hindrance of PAC-M II samples. Similar observations have been published by Støvring Mortensen et al (Støvring Mortensen et al, 2022), suggesting that permeation of FITC dextrans in stored porcine jejunal mucus is similar to permeation through freshly isolated porcine jejunal mucus.…”

“…Støvring Mortensen et al made similar conclusions in their assessment of various storage conditions (fresh, − 20 • C, or at − 80 • C directly or after snap freezing in liquid nitrogen, or freeze-dried and reconstituted with distilled water). They found that the various storage conditions have negligible effect on the barrier properties of porcine native jejunal mucus compared to fresh secretion (Støvring Mortensen et al, 2022). In the present study, we confirmed that the same applies to PACM II , demonstrating the flexibility and applicability of the porcine artificial colonic mucus in routine testing.…”

“…A recent study of FITC-dextran (4K, 40K, and 150K) diffusion in porcine jejunal mucus also reports decreasing apparent permeability values with increasing molecular weight (Støvring Mortensen et al, 2022). The decrease in diffusivity values is not proportional to the increase in molecular weight, as shown in studies on porcine gastric mucus (Desai, Mutlu, and Vadgama, 1992), porcine jejunal mucus (Støvring Mortensen et al, 2022), and human cystic fibrosis sputum (Braeckmans et al, 2003). Thus, for molecules with molecular weights above the cut-off of the mucus mesh, diffusion is substantially delayed.…”

“…The authors observed consistent retardation of solute flux in the mucus with increasing molecular weight, although no absolute molecular weight cut-off for the phenomenon. A recent study of FITC-dextran (4K, 40K, and 150K) diffusion in porcine jejunal mucus also reports decreasing apparent permeability values with increasing molecular weight (Støvring Mortensen et al, 2022). The decrease in diffusivity values is not proportional to the increase in molecular weight, as shown in studies on porcine gastric mucus (Desai, Mutlu, and Vadgama, 1992), porcine jejunal mucus (Støvring Mortensen et al, 2022), and human cystic fibrosis sputum (Braeckmans et al, 2003).…”