Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol

Gu, Min; Song, Sang Woo; Lee, In Kyu; Ko, Seongyeol; Han, Seung Eun; Bae, Sung-Hwan; Ji, Sang Yun; Park, Byung‐Chul; Song, Ki-Duk; Lee, Hak-Kyo; Han, Seung Hyun; Yun, Cheol‐Heui

doi:10.1186/s13567-016-0309-1

Cited by 47 publications

(37 citation statements)

References 38 publications

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Section: Discussionmentioning

confidence: 99%

“…Regarding the influence of DON on TJ proteins of IPEC-J2, literature reports exist with respect to ZO-1, claudin-3 and occludin [ 10 , 45 , 82 , 83 ]. Gu et al [ 83 ] reported ZO-1 reductions after 72 h basolateral exposure to DON (6.73 µM). Others demonstrated—in accordance with our finding—no [ 45 ] or only slight [ 10 , 82 ] DON-induced (6.73 µM) ZO-1 reductions in apically treated IPEC-J2, but strong effects in cells exposed basolaterally for 72 h. While our finding of a DON-induced claudin-3 decrease is in accordance with Gu et al [ 83 ], others report no effect of DON on claudin-3 in apically treated IPEC-J2 [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Gu et al [ 83 ] reported ZO-1 reductions after 72 h basolateral exposure to DON (6.73 µM). Others demonstrated—in accordance with our finding—no [ 45 ] or only slight [ 10 , 82 ] DON-induced (6.73 µM) ZO-1 reductions in apically treated IPEC-J2, but strong effects in cells exposed basolaterally for 72 h. While our finding of a DON-induced claudin-3 decrease is in accordance with Gu et al [ 83 ], others report no effect of DON on claudin-3 in apically treated IPEC-J2 [ 10 ]. Finally, the effect of occludin was studied in DON-treated IPEC-J2, showing—in contrast to our finding—a decrease of the TJ component at 6.73 µM after 48 h [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

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Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network

Springler¹,

Hessenberger²,

Schatzmayr³

et al. 2016

Toxins

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Deoxynivalenol (DON), produced by the plant pathogens Fusarium graminearum and Fusarium culmorum, is one of the most common mycotoxins, contaminating cereal and cereal-derived products. Although worldwide contamination of food and feed poses health threats to humans and animals, pigs are particularly susceptible to this mycotoxin. DON derivatives, such as deepoxy-deoxynivalenol (DOM-1), are produced by bacterial transformation of certain intestinal bacteria, which are naturally occurring or applied as feed additives. Intestinal epithelial cells are the initial barrier against these food- and feed-borne toxins. The present study confirms DON-induced activation of MAPK p44/42 and inhibition of p44/42 by MAPK-inhibitor U0126 monoethanolate. Influence of DON and DOM-1 on transepithelial electrical resistance (TEER), viability and expression of seven tight junction proteins (TJ), as well as the potential of U0126 to counteract DON-induced effects, was assessed. While DOM-1 showed no effect, DON significantly reduced TEER of differentiated IPEC-J2 and decreased expression of claudin-1 and -3, while leaving claudin-4; ZO-1, -2, and -3 and occludin unaffected. Inhibition of p44/42 counteracted DON-induced TEER decrease and restored claudin-3, but not claudin-1 expression. Therefore, effects of DON on TEER and claudin-3 are at least partially p44/42 mediated, while effects on viability and claudin-1 are likely mediated via alternative pathways.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network

Springler¹,

Hessenberger²,

Schatzmayr³

et al. 2016

Toxins

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show abstract

“…4c). These effects could notably be explained by the ability of A. muciniphila to activate TLR2 via Amuc_1100, as this receptor can regulate various tight-junction proteins including Occludin and Claudin 3 31,32 . In the colon, untreated HFD-fed mice had a higher Ocln expression as compared to the other groups,…”

mentioning

confidence: 99%

A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice

Plovier

Everard

Druart

et al. 2016

Nat Med

1,611

1,381

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Obesity and type 2 diabetes are associated with low-grade inflammation and specific 34 changes in gut microbiota composition [1][2][3][4][5][6][7] . We previously demonstrated that administration 35 of Akkermansia muciniphila prevents the development of obesity and associated 36 complications 8 . However, its mechanisms of action remain unclear, whilst the sensitivity of 37 A. muciniphila to oxygen and the presence of animal-derived compounds in its growth 38 medium currently limit the development of translational approaches for human medicine 9 . 39Here we addressed these issues by showing that A. muciniphila retains its efficacy when Akkermansia muciniphila is one of the most abundant members of the human gut 53 microbiota, representing between 1 and 5% of our intestinal microbes 10,11 to improve glucose intolerance and insulin resistance regardless of the growth medium used and 71 independently of food intake ( Fig. 1a-g). 72 We previously showed that autoclaving A. muciniphila abolished its beneficial effects 8 . (Fig. 1a-c and Supplemental Fig. 1a-c). In both sets of 81 experiments, we found that mice treated with pasteurized A. muciniphila displayed a much lower 82 glucose intolerance and insulin concentration when compared to the HFD group, resulting in a 83 lower insulin resistance (IR) index (Fig. 1d-g and Supplemental Fig. 1d-g). Treatment with 84 pasteurized A. muciniphila also led to greater goblet cell density in the ileum when compared to 85 ND-fed mice (Fig. 1h), suggesting a higher mucus production, while normalizing the mean 86 adipocyte diameter (Fig. 2a-b) and significantly lowering plasma leptin when compared to HFD-87 fed mice (Fig. 2c). These effects were not observed in mice treated with live A. muciniphila. A 88 similar trend could be observed for plasma resistin (Supplemental Fig. 1h), thereby suggesting 89 improved insulin sensitivity, while plasma adiponectin remained unaffected in all conditions 90 (Supplemental Fig. 1i). We found that mice treated with pasteurized A. muciniphila had a higher 91 fecal caloric content when compared to all other groups (Fig. 2d), suggesting a lower energy (Fig. 2e-g). This resulted in a normalization of the HFD-induced shift of 37% with the 104 pasteurized bacterium, and 17% with the live bacterium ( Fig. 2f). 105By comparing the metabolic profiles of the different groups, we found that the shift 106 induced by pasteurized A. muciniphila was mainly associated with trimethylamine (TMA) and TMA to TMAO, a metabolite associated with atherosclerosis 19,20 . While exposure to a HFD led 114 to a two-fold higher Fmo3 expression when compared to ND-fed mice, treatment with 115 pasteurized A. muciniphila reversed this effect (Fig. 2j) Fmo3 expression were not associated with a modification of plasma TMA and TMAO, as all 121 HFD-fed group displayed similar concentrations for both metabolites (Fig. 2k,l) (Fig. 3a), but not cells expressing TLR5, TLR9 or the NOD2 receptor (Fig. 3b-131 d). 132Genomic and proteomic analyses of A. muciniphila identified p...

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“…Toll-like receptors 4 (TLR4) is a trans-membrane proteins, an important member of the toll-like receptors family, which detect the PRRs and activate the NF-κB (intracellular signaling pathway), which ultimately activate immune response by producing pro-inflammatory cytokines (Fig. 2) (Lebeer et al, 2010;Gu et al, 2016). Some studies exposed that IgA is the dominant immunoglobulin in the intestine and plays a key role in immunity (Mahfuz et al, 2017).…”

Section: Associated Immune Responsesmentioning

confidence: 99%

The Potential Role of Probiotics (nutraceuticals) in Gut Health of Domestic Animals; an Alternative to Antibiotic Growth Promoters

Nawab

Liu

et al. 2019

J Hellenic Vet Med Soc

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The term gut health is currently becoming more important for domestic animals including poultry. Gut health refers to the fundamental organ system which covers multiple positive functions like effective digestion, stabilizing intestinal microbiota, gut pH and modulation of effective immune response. Gut health depends on proper balance of microbial population. A wide range of feed and pathogen associated factors influence this balance, and adversely affect the animal health status and production performance. Antibiotic stimulators have been used in farm animals to achieve maximum production. But drug resistance and residual effects of antibiotics in animal products (milk, meat and egg etc.) have raised serious issues in human life. Therefore, The European Union (EU) has strictly banned the application of antibiotic stimulators in livestock nutrition in several others countries including China. As a result, an alternative to antibiotic growth promoters are required to support the profitable and sustainable animal production system. Probiotics as nutraceuticals has been categorized as an alternative natural feed supplement for commercial utilization. Such products have been recognized as safe feed additives in animal industry. Very few studies have comparatively described the effect of probiotics on gut health of domestic animals. Therefore, the aim of this review is not only to explore the beneficial effects of probiotics in improving gut health of domestic animals as an alternative to antibiotic growth promoters, but also to evaluate the probiotics associated health and risk factors, and to provide comprehensive scientific information for researchers, scientists and commercial producers.

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Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol

Cited by 47 publications

References 38 publications

Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network

Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network

A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice

The Potential Role of Probiotics (nutraceuticals) in Gut Health of Domestic Animals; an Alternative to Antibiotic Growth Promoters

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