Baru Pulp (Dipteryx alata Vogel): Fruit from the Brazilian Savanna Protects against Oxidative Stress and Increases the Life Expectancy of Caenorhabditis elegans via SOD-3 and DAF-16

Leite, Natasha Rios; Araújo, Leandro Coelho de; Rocha, Paola dos Santos da; Agarrayua, Danielle Araújo; Ávila, Daiana Silva; Carollo, Carlos Alexandre; Silva, Denise Brentan; Estevinho, Letí­cia M.; Souza, Kely de Picoli; Santos, Edson Lucas dos

doi:10.3390/biom10081106

Cited by 26 publications

(4 citation statements)

References 41 publications

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“…In addition, the TJ375 strain was exposed or not to 37 °C for 2 h and recovered for 2–3 h at 20 °C. Subsequently, 10 nematodes in each experimental and control group were randomly selected to be anesthetized with 1mM hydrochloride levamisole [ 68 ] and were fixed on a 2% agarose pad. All the GFP fluorescence assays were performed with the filter (excitation wavelength 485 nm, emission wavelength 535 nm) and an Axio Scrope A1 fluorescence microscope (Carl Zeiss AG, Jena, Germany) connected to Zen Blue Software’s AxioCam 503 color digital camera for image acquisition and analysis, and the relative fluorescence intensity of the entire body was determined using ImageJ software.…”

Section: Methodsmentioning

confidence: 99%

Planococcus maritimu ML1206 Strain Enhances Stress Resistance and Extends the Lifespan in Caenorhabditis elegans via FOXO/DAF-16

Lin

Jin

et al. 2022

Marine Drugs

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The antioxidant effect of probiotics has been widely recognized across the world, which is of great significance in food, medicine, and aquaculture. There are abundant marine microbial resources in the ocean, which provide a new space for humans to explore new probiotics. Previously, we reported on the anti-infective effects of Planococcus maritimu ML1206, a potential marine probiotic. The antioxidant activity of ML1206 in C. elegans was studied in this paper. The study showed that ML1206 could improve the ability of nematodes to resist oxidative stress and effectively prolong their lifespan. The results confirmed that ML1206 could significantly increase the activities of CAT and GSH-PX, and reduce the accumulation of reactive oxygen species (ROS) in nematodes under oxidative stress conditions. In addition, ML1206 promoted DAF-16 transfer to the nucleus and upregulated the expression of sod-3, hsp-16.2, and ctl-2, which are downstream antioxidant-related genes of DAF-16. Furthermore, the expression of the SOD-3::GFP and HSP-16.2::GFP was significantly higher in the transgenic strains fed with ML1206 than that in the control group fed with OP50, with or without stress. In summary, these findings suggest that ML1206 is a novel marine probiotic with an antioxidant function that stimulates nematodes to improve their defense abilities against oxidative stress and prolong the lifespan by regulating the translocation of FOXO/DAF-16. Therefore, ML1206 may be explored as a potential dietary supplement in aquaculture and for anti-aging and antioxidant purposes.

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Section: Methodsmentioning

confidence: 99%

Planococcus maritimu ML1206 Strain Enhances Stress Resistance and Extends the Lifespan in Caenorhabditis elegans via FOXO/DAF-16

Lin

Jin

et al. 2022

Marine Drugs

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“…Antioxidant activity [70] Phenolic compounds (total quantification) In vivo antioxidant activity [71] Phenols and terpenes Antioxidant activity and Caenorhabditis elegans life expectancy increase [72] Eschweilera nanat Rutin and hyperoside Antioxidant activity [73] Eugenia dysenterica…”

Section: Caryocar Brasiliensementioning

confidence: 99%

New Therapeutic Strategies for Obesity and Its Metabolic Sequelae: Brazilian Cerrado as a Unique Biome

Monteiro-Alfredo,

Macedo,

de Picoli Souza

et al. 2023

IJMS

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Brazil has several important biomes holding impressive fauna and flora biodiversity. Cerrado being one of the richest ones and a significant area in the search for new plant-based products, such as foods, cosmetics, and medicines. The therapeutic potential of Cerrado plants has been described by several studies associating ethnopharmacological knowledge with phytochemical compounds and therapeutic effects. Based on this wide range of options, the Brazilian population has been using these medicinal plants (MP) for centuries for the treatment of various health conditions. Among these, we highlight metabolic diseases, namely obesity and its metabolic alterations from metabolic syndrome to later stages such as type 2 diabetes (T2D). Several studies have shown that adipose tissue (AT) dysfunction leads to proinflammatory cytokine secretion and impaired free fatty acid (FFA) oxidation and oxidative status, creating the basis for insulin resistance and glucose dysmetabolism. In this scenario, the great Brazilian biodiversity and a wide variety of phytochemical compounds make it an important candidate for the identification of pharmacological strategies for the treatment of these conditions. This review aimed to analyze and summarize the current literature on plants from the Brazilian Cerrado that have therapeutic activity against obesity and its metabolic conditions, reducing inflammation and oxidative stress.

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“…Увеличение здоровой продолжительности жизни тесно связано с поздним возникновением хронических заболеваний. Ученые предполагают, что одной из причин, стимулирующей процессы старения, а именно развитие рака, атеросклероза, сердечно-сосудистых, нейродегенеративных заболеваний и т.п., является окислительный стресс (свободнорадикальная теория старения) [3,11]. Следовательно, профилактика старения возможна за счет приема препаратов, подавляющих/уменьшающих негативное воздействие хронических заболеваний, подавляющих развитие окислительного стресса.…”

Section: Introductionunclassified

Evaluation of the Geroprotective Effect of Squalene on the Caenorhabditis Elegans Model

Vesnina

Dolganyuk

Dmitrieva

et al. 2022

SJLSA

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Background. Increasing healthy life expectancy is a priority in modern healthcare. Squalene, a triterpene exhibiting antioxidant, anti-inflammatory and other properties, is a promising candidate for geroprotectors. Purpose. Assess the activity of squalene, that is, the possibility of its further use as a geroprotector. Materials and methods. Squalene was purchased from Sigma-Aldrich (USA) for research. Squalene solutions were prepared with concentrations of 10, 50, 100, 200, 400, 800, and 1600 µmol/L (solvent dimethyl sulfoxide—DMSO). The work was carried out on the basis of the Laboratory for Biotesting of Natural Nutraceuticals (KemGU, Russia). Antioxidant activity was assessed by capturing ABTS radical cations in comparison with the activity of ascorbic acid. The model organism Caenorhabditis elegans N2 Bristol was purchased from the Laboratory for the Development of Innovative Medicines and Agrobiotechnologies of the Moscow Institute of Physics and Technology. To assess the stress resistance of nematodes during cultivation, conditions of thermal (33°C) and oxidative (adding 15 μl of 1 M paraquat, 20°C) stress were created for 24 and 48 h. conditions (20 °C) for 61 days. The statistical significance of the results was assessed using Student’s t-test (in Microsoft Office Excel 2007). Lifespan data were assessed using a Kaplan-Meier survival plot combined with a log-rank test (Mantel-Cox). Data processing was carried out using the OASIS online application (https://sbi.postech.ac.kr/oasis/). Results. The results showed that squalene solutions did not exhibit antioxidant properties; did not trap radical cations with ABTS. A solution of squalene at a concentration of 10 μM contributed to an increase in the stress resistance of nematodes. Under conditions of oxidative stress, the survival of nematodes during cultivation for 24 hours is 3.1% higher than in the control. Under conditions of heat stress during cultivation for 48 hours, the survival rate is higher by 11.3%. Squalene solutions did not affect the lifespan of nematodes. Conclusion. Presumably, the antioxidant activity of squalene is expressed not in the capture of free radicals, but in the ability to act on biomolecules that lead to oxidative stress. A squalene solution with a concentration of 10 μM can reduce the negative impact of one of the factors causing premature aging. In order to assess the possibility of using squalene as a geroprotector without relying on cation-radical capture, further studies are needed to study the impact of the substance on other targets, mechanisms of action related to the occurrence of age-associated diseases. For example, analysis of the effect of squalene solutions on the growth rate of C. elegans larvae, the ability to influence the expression of antioxidant defense genes, the presence of anti-inflammatory and other properties.

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Baru Pulp (Dipteryx alata Vogel): Fruit from the Brazilian Savanna Protects against Oxidative Stress and Increases the Life Expectancy of Caenorhabditis elegans via SOD-3 and DAF-16

Cited by 26 publications

References 41 publications

Planococcus maritimu ML1206 Strain Enhances Stress Resistance and Extends the Lifespan in Caenorhabditis elegans via FOXO/DAF-16

Planococcus maritimu ML1206 Strain Enhances Stress Resistance and Extends the Lifespan in Caenorhabditis elegans via FOXO/DAF-16

New Therapeutic Strategies for Obesity and Its Metabolic Sequelae: Brazilian Cerrado as a Unique Biome

Evaluation of the Geroprotective Effect of Squalene on the Caenorhabditis Elegans Model

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