Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore

Small‐Molecule Quenchers for Förster Resonance Energy Transfer: Structure, Mechanism, and Applications

“…Detect caspase-3 and survivin mRNA, [26] Uracil DNA glycosylases (Ung) [35] /MMP-3, [30] /MMPs [39] /MMP-3, [31] hypoxic tissues [41] Detect miR-21, [44] Zika-specific DNA in human blood serum, [45] the activity of anti-digoxigenin (Dig) antibodies, [46] intracellular target mRNA [47] /caspase-3 [48] IQ 4 587 520-706 56 000 -/--/-Eclipse 522 460-570 -/-FAM/aminoacridine Detect Guignardia citricarpa, [50] human immunodeficiency virus type 1 (HIV-1) in plasma [51] /single base pair mutations [52] TAMRA 544 470-560 85 000 FAM Detect AFB1 in beer, wine samples, [58] the mutation level of Kisten rat sarcoma (KRAS) viral gene [59] QSY7 560 500-600 92 000 Cy3/BODIPY/fluorescein (92 300; 518) Detect Cathepsin B (CaB), [67] caspase-3 [75] /E. coli outer membrane protease (OmpT) [72] /protein/ribosome conformation [76] QSY9 562 500-600 88 000…”

“…The TaqMan probe was modified at 5′ end with FAM and labeled at the 3′ end with the Eclipse dark quencher, which is able to effectively detect fungus Guignardia citricarpa from imported diseased citrus fruit [50] . Eclipse dark quencher has also been used in the detection of human immunodeficiency virus type 1 (HIV‐1) in plasma [51] and DNA single base pair mutations [52] …”

“…[50] Eclipse dark quencher has also been used in the detection of human immunodeficiency virus type 1 (HIV-1) in plasma [51] and DNA single base pair mutations. [52]…”

Acridinyl methyl esters as photoactive precursors in the release of neurotransmitteramino acids

“…31,32 In strongly hydrogen bonding solvents, such as water, lifetimes over 10 ns have been reported. 20,2130,[33][34] The photocleavage is expected to proceed via the formation of an ion pair, which can undergo either a recombination to the initial ester or complete the cleavage with formation of photoproducts (Scheme 1). 35 These processes should influence the fluorescence decay of the Acm moiety and as the different species can be spectrally different, the ability to obtain time-resolved emission spectra is a powerful technique by which to characterise and elucidate the processes occurring in this type of system.…”

Bio-nanogate controlled enzymatic reaction for virus sensing

“…In recent years, a considerable amount of effort has been devoted to the design and fabrication of artificial nanoswitches/nanogates with various synthesized or natural molecules for diverse applications (Browne and Feringa, 2006;Beissenhirtz and Willner, 2006;Liedl et al, 2007;Wu et al, 2008;Ferapontova et al, 2008;Liu and Liu, 2009;McGuinness et al, 2010aMcGuinness et al, , 2010bGuerra et al, 2011;Chen et al, 2011;Wang et al, 2012). The recent developments in DNA-based nanotechnology have shown unprecedented potential towards constructing functional nanodevices due to the unique molecular recognition capabilities and structural features (Seeman, 2003;Aldaye et al, 2008;Sharma et al, 2009;He et al, 2008;Nykypanchuk et al, 2008;Yin et al, 2008;Schlossbauer et al, 2010).…”