Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine

Abstract

According to French Paradox, red wine was famous for the potential effects on coronary heart disease (CHD), but the specific compounds against CHD were unclear. Therefore, screening and characterization of bioactive compounds from red wine was extremely necessary. In this paper, the multi-activity integrated strategy was developed and validated to screen, identify and quantify active compounds from red wine by using ultra high performance liquid chromatography-fraction collector (UHPLC-FC), ultra fast liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UFLC-Q-TOF/MS) and bioactive analysis. UHPLC-FC was employed to separate and collect the components from red wine, which was further identified by UFLC-Q-TOF/MS to acquire their structural information. Furthermore, the active fractions were tested for antioxidant activity, inhibitory activity against thrombin and lipase activities in vitro by the activity screening kit. As the results, there were 37 fractions had antioxidant activity, 22 fractions had thrombin inhibitory activity and 28 fractions had lipase inhibitory activity. Finally, 77 active components from red wine were screened and 12 ingredients out of them were selected for quantification based on the integration of multi-activity. Collectively, the multi-activity integrated strategy was helpful for the rapid and effective discovery of bioactive components, which provided reference for exploring the health care function of food.

Cytotoxic and Antioxidant Activities of Imine Analogs of Trans-Resveratrol towards Murine Neuronal N2a Cells

“…RSV is implied in the French paradox [ 2 , 3 ] and in the Mediterranean diet [ 4 , 5 ]. The interest aroused by RSV since the 1990s [ 6 ] is especially due to its pleiotropic antifungal and therapeutic activities in the treatment of inflammatory diseases and some cancer.…”

A SARS-CoV-2 Mprofluorescent sensor for exploring pharmacodynamic substances from traditional Chinese medicine

“…Structures of 61 compounds in Xuebijing injection were qualitatively identified through SCIEX OS data processing software by searching the chemistry database (https://massbank.eu/ MassBank/, https://pubchem.ncbi.nlm.nih.gov/), standards and related literature, as shown in Table S3, † where the peak number, retention time, MS/MS fragment ion information and identification results of each component of Xuebijing injection are listed. [53][54][55][56][57][58][59][60][61] The activities of protocatechualdehyde, chlorogenic acid, hydroxysafflower yellow A, caffeic acid, isoquercetin and pentagalloylglucose were further verified according to the activity of the existing reference products and active compounds (Fig. S20 †).…”