In a baseline study, we investigated microplastic contamination in fish from the Pinang and Kerian Rivers in Northwest Peninsular Malaysia. In recognition of the growing concern over microplastic pollution in aquatic environments, we aimed to assess the abundance and characteristics of microplastics ingested by various fish species. Fish samples were collected from local fishermen, followed by a digestion process using 10% potassium hydroxide (KOH). Microplastics were isolated and analyzed through visual examination and attenuated total reflectance Fourier transform infrared spectroscopy. The results revealed a high prevalence of microplastics, with Johnius borneensis and Oreochromis sp. exhibiting the highest abundance, averaging 48.6 and 42.8 microplastics/g, respectively. The predominant shapes were fibers (55.6%) and fragments (25.9%), with colors primarily transparent (48.19%) and black (30.12%). Our results indicate significant contamination levels in freshwater fish, emphasizing the need for further research and effective mitigation strategies. These findings provide crucial baseline data on microplastics in Malaysian freshwater ecosystems. Environ Toxicol Chem 2024;00:1–10. © 2024 SETAC