Baseline sensitivity and toxic actions of boscalid against Sclerotinia sclerotiorum

Hu, Simin; Zhang, Jun; Zhang, Yuchao; He, Shun; Zhu, Fuxing

doi:10.1016/j.cropro.2018.04.004

Cited by 33 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, fungicides have been used as the auxiliary method for controlling SSR in practice [5]. The benzimidazole and dicarboximide fungicides were the most efficient fungicides in controlling SSR [6]. However, the continuous use of these fungicides with high concentration can amplify the resistant level of phytopathogens [7,8,9].…”

Section: Introductionmentioning

confidence: 99%

Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean

Liu

Zhuang

et al. 2019

Microorganisms

View full text Add to dashboard Cite

The present study was conducted to examine the influence of a pathogen Sclerotinia sclerotiorum (Lib.) de Bary on the actinobacterial community associated with the soybean roots. A total of 70 endophytic actinobacteria were isolated from the surface-sterilized roots of either healthy or diseased soybeans, and they were distributed under 14 genera. Some rare genera, including Rhodococcus, Kribbella, Glycomyces, Saccharothrix, Streptosporangium and Cellulosimicrobium, were endemic to the diseased samples, and the actinobacterial community was more diverse in the diseased samples compared with that in the heathy samples. Culture-independent analysis of root-associated actinobacterial community using the high-throughput sequencing approach also showed similar results. Four Streptomyces strains that were significantly abundant in the diseased samples exhibited strong antagonistic activity with the inhibition percentage of 54.1–87.6%. A bioactivity-guided approach was then employed to isolate and determine the chemical identity of antifungal constituents derived from the four strains. One new maremycin analogue, together with eight known compounds, were detected. All compounds showed significantly antifungal activity against S. sclerotiorum with the 50% inhibition (EC50) values of 49.14–0.21 mg/L. The higher actinobacterial diversity and more antifungal strains associated with roots of diseased plants indicate a possible role of the root-associated actinobacteria in natural defense against phytopathogens. Furthermore, these results also suggest that the root of diseased plant may be a potential reservoir of actinobacteria producing new agroactive compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean

Liu

Zhuang

et al. 2019

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Owing to its unique mode of action, boscalid is highly efficacious for control of a variety of plant diseases such as powdery mildew, gray mold, leaf spot, and stem rot. Baseline sensitivity and control efficacy of boscalid against S. sclerotiorum have been reported recently (Hu et al 2018;Liu et al 2018). In order to judiciously apply boscalid for control of SSR, the stimulatory effects of this fungicide on mycelial growth and virulence of S. sclerotiorum need to be investigated.…”

mentioning

confidence: 99%

Stimulatory Effects of Boscalid on Virulence of Sclerotinia sclerotiorum Indicate Hormesis May Be Masked by Inhibitions

Zhang

et al. 2020

Plant Disease

Self Cite

View full text Add to dashboard Cite

Hormetic effects of fungicides on phytopathogens are of great importance for proper application of fungicides. The aim of the present study was to investigate the stimulatory effects of the fungicide boscalid on mycelial growth and virulence of the devastating plant pathogen Sclerotinia sclerotiorum. Boscalid in potato dextrose agar (PDA) at a dosage range from 0.0005 to 0.002 μg/ml exerted statistically significant (P ≤ 0.015) stimulations on mycelial growth of S. sclerotiorum, and the maximum stimulation magnitudes were 5.55 ± 0.73% (mean ± SD) for the four isolates tested. Boscalid in PDA at 0.02 μg/ml inhibited mycelial growth of isolates HLJ3H and HLJ4H by 15.0 and 8.9%, respectively. However, after the growth-inhibited mycelia were inoculated on rapeseed leaves, isolates HLJ3H and HLJ4H exhibited virulence stimulations of 8.7 and 17.8%, respectively, indicating that hormesis may be masked by inhibitions. Boscalid sprayed at 0.0001 to 0.1 μg/ml on detached rapeseed leaves had significant (P ≤ 0.041) stimulations on virulence of S. sclerotiorum, and the maximum stimulation magnitudes were 17.90 ± 5.94% (mean ± SD) for the four isolates tested. Experiments on 12 isolates with different levels of virulence showed there was a negative correlation (R = –0.663, P = 0.019) between the maximum virulence stimulation magnitude and virulence of S. sclerotiorum in the absence of fungicide. Boscalid at stimulatory concentrations had no significant effect on the expression levels of three virulence-associated genes that encode cutinase (SsCut), polygalacturonase (SsPG1), and oxaloacetate acetylhydrolase (SsOah1). The molecular mechanisms for hormetic effects of boscalid on S. sclerotiorum remain to be studied in the future.

show abstract

“…Efficient chemical control of SSR relies on prophylactic application of fungicides, since curative spraying does not revert yield losses despite being effective in reducing the inoculum potential for subsequent crops. The intensive long-term fungicide-based management strategies for the control of this disease resulted in the development of resistant S. sclerotiorum strains toward many active ingredients (such as carbendazim, dimetachlone, and thiophanate-methyl), demanding constant baseline sensitivity studies to monitor the field efficacy of chemicals [23][24][25][26][27][28][29]. The need to introduce alternative molecules to control this devastating pathogen, allied to environmental and food securities, opened the market for bioproducts.…”

Section: Situation Of Sclerotinia Stem Rot In Brazilmentioning

confidence: 99%

Trichodermaas a Biocontrol Agent againstSclerotiniaStem Rot or White Mold on Soybeans in Brazil: Usage and Technology

Juliatti¹,

Rezende²,

Juliatti³

et al. 2019

Trichoderma - The Most Widely Used Fungicide

View full text Add to dashboard Cite

Biological control agents are alternatives to chemical pesticides in the management of plant diseases. Currently, hundreds of bioproducts are commercially available in international market varying mainly in antagonistic microorganisms and formulation. We screened four Trichoderma-based products as to their efficacy in controlling Sclerotinia stem rot (SSR) under protected and field environments and their effect on soybean seeds' sanity and physiological qualities. We also tested application technologies through seed microbiolization and foliar spraying to deliver the microorganisms, and their compatibility with chemical fungicides. In vitro assays showed that all Trichoderma strains were antagonistic to S. sclerotiorum evidencing hyperparasitic activity. Moreover, the bioproducts reduced fungi incidence on soybean seeds, promoted faster seedling emergence and did not hamper seeds' vigor. Increases of 14 and 37% were registered for root length and shoot fresh weight over that of the untreated control indicating potential application of the bioproducts as soybean growth promoters. Thiophanate-methyl and procymidone were the most compatible, without drastically affecting spore germination or mycelium growth. Under field conditions, all Trichoderma strains reduced SSR incidence and increased soybean grain yield. Formulation interferes on bioproducts' viability and efficacy deserving special attention upon development.

show abstract

Baseline sensitivity and toxic actions of boscalid against Sclerotinia sclerotiorum

Cited by 33 publications

References 23 publications

Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean

Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean

Stimulatory Effects of Boscalid on Virulence of Sclerotinia sclerotiorum Indicate Hormesis May Be Masked by Inhibitions

Trichodermaas a Biocontrol Agent againstSclerotiniaStem Rot or White Mold on Soybeans in Brazil: Usage and Technology

Contact Info

Product

Resources

About