Basement membrane carbohydrate as a target for bacterial adhesion: binding of type I fimbriae of Salmonella enterica and Escherichia coli to laminin

Abstract

Adherence of type-1-fimbriate Salmonella enterica and Escherichia coli to immobilized proteins of the extracellular matrix and reconstituted basement membranes was studied. The type-1-fimbriate strain SH401 of S. enterica serovar Enteritidis showed good adherence to laminin, whereas the adherence to fibronectin, type I, type III, type IV or type V collagens was poor. Only minimal adherence to the matrix proteins was seen with a non-fimbriate strain of S. enterica serovar Typhimurium. A specific and mannoside-inhibitable adhesion to laminin was exhibited by the recombinant E. coli strain HB101(pISF101) possessing fim genes of Typhimurium. Adherence to laminin of strain SH401 was inhibited by Fab fragments against purified SH401 fimbriae, and a specific binding to laminin, of the purified fimbriae, was demonstrated using fimbriae-coated fluorescent microparticles. Periodate treatment of laminin abolished the bacterial adhesion as well as the fimbrial binding. Specific adhesion to immobilized laminin was also shown by the type-1-fimbriate E. coli strain 2131 and the recombinant strain E. coli HB101(pPKL4) expressing the cloned type-1-fimbriae genes of E. coli. Adhesion to laminin of strain HB101(pPKL4) was inhibited by mannoside, and no adherence was seen with the fimH mutant E. coli HB101(pPKL5/pPKL53) lacking the fimbrial lectin subunit. The type-1 fimbriate strains also adhered to reconstituted basement membranes from mouse sarcoma cells and human placenta. Adhesion of strains HB101(pISF101) and HB101(pPKL4) to both basement membrane preparations was inhibited by mannoside. We conclude that type-1 fimbriae of S. enterica and E. coli bind to oligomannoside chains of the laminin network in basement membranes.

Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria

“…These finding is in agreement with earlier reports by Huguette et al (1996), Alaa and Adnan (2011) which both found that the fimA gene contains sequences unique to Salmonella isolates and demonstrated that this gene is suitable for PCR target for the detection of Salmonella strains. Bacterial adherence (Kurkkonen et al, 1993) is considered to be a baseline requirement for infection, and there is evidence that many bacteria have surface appendages, such as pili or fimbriae (Clegg et al, 1985;Aslanzadeh and Paulissen, 1992), that facilitate their binding to specific receptors on the epithelial cell surface, or intestinal mucus. Although certain types of Escherichia coli fimbriae are known to be important for virulence, only type 1 fimbriae (fimA) have been implicated in Salmonella pathogenicity.…”

“…Others play pivotal roles in the survival of organism in the host system-mgtC (Magnesium transport C) (Blanc-Potard and Groisman 1997) or in the actual manifestation of pathogenic processes for example stn (Salmonella toxin), pip A, B, D (Marcus et al, 2000). Bacterial adherence (Kurkkonen et al, 1993) is considered to be a prerequisite for infection, and there are evidences that many bacteria have surface processes, such as fimbriae (Clegg et al, 1985;Aslanzadeh and Paulissen, 1992) or pili, that facilitate attachment to particular receptors on the epithelial cell surface, to urinary tract or intestinal mucus. Although certain strains of E. coli fimbriae are known to be important for virulence, only type 1 fimbriae (fimA) have been implicated in Salmonella pathogenicity.…”

CEACAM6 acts as a receptor for adherent-invasive E. coli, supporting ileal mucosa colonization in Crohn disease

“…Several glycosylated receptors for type 1 pili have been documented, such as glycosylphosphatidylinositol-anchored (GPI-anchored) protein CD48, Tamm-Horsfall protein (THP), and various family members of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) (16)(17)(18)(19)(20). Western blot analysis of total protein extract from ileal specimens of 9 CD patients, taken in both involved and uninvolved areas, and of 9 controls showed a strong expression of CEACAM5 (also called CEA or CD66e) and CEACAM6 (also called CD66c or non-crossreacting antigen [NCA]) in the ileal mucosa of CD patients in both involved and uninvolved areas ( Figure 3A).…”

Distribution, gene sequence and expressionin vivoof the plasmid encoded fimbrial antigen ofSalmonellaserotype Enteritidis

“…However [19]. The SEF17 fimbrial structure, which was demonstrated to share 70°% identity with the 'curli' fimbrial structure of E. coli [20], generated an aggregative phenotype and bound fibronectin [21][22][23].…”