Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen

Kuhnert, Peter; Scholten, Edzard; Haefner, Stefan; Mayor, Désirée; Frey, Joachim

doi:10.1099/ijs.0.011809-0

Cited by 93 publications

(64 citation statements)

References 31 publications

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“…Each of the two subclades has its own distinct USS, the Hin type and Apl type, respectively, and the species of each subclade preferentially take up DNA containing the corresponding USS (33). Gallibacterium is positioned as the deepest branch in Pasteurellaceae phylogenies and thus is a sister group to the rest of the Pasteurellaceae and not a member of either subclade (22,23).…”

Section: Identification Of a Putative Competence Regulon In G Anatismentioning

confidence: 99%

Natural Transformation of Gallibacterium anatis

Kristensen

Sinha

Boyce

et al. 2012

Appl Environ Microbiol

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ABSTRACTGallibacterium anatisis a pathogen of poultry. Very little is known about its genetics and pathogenesis. To enable the study of gene function inG. anatis, we have established methods for transformation and targeted mutagenesis. The genusGallibacteriumbelongs to thePasteurellaceae, a group with several naturally transformable members, includingHaemophilus influenzae. Bioinformatics analysis identifiedG. anatishomologs of theH. influenzaecompetence genes, and natural competence was induced inG. anatisby the procedure established forH. influenzae: transfer from rich medium to the starvation medium M-IV. This procedure gave reproducibly high transformation frequencies withG. anatischromosomal DNA and with linearized plasmid DNA carryingG. anatissequences. Both DNA types integrated into theG. anatischromosome by homologous recombination. Targeted mutagenesis gave transformation frequencies of >2 × 10−4transformants CFU−1. Transformation was also efficient with circular plasmid containing noG. anatisDNA; this resulted in the establishment of a self-replicating plasmid. Nine diverseG. anatisstrains were found to be naturally transformable by this procedure, suggesting that natural competence is common and the M-IV transformation procedure widely applicable for this species. TheG. anatisgenome is only slightly enriched for the uptake signal sequences identified in other pasteurellaceaen genomes, butG. anatisdid preferentially take up its own DNA over that ofEscherichia coli. Transformation by electroporation was not effective for chromosomal integration but could be used to introduce self-replicating plasmids. The findings described here provide important tools for the genetic manipulation ofG. anatis.

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Section: Identification Of a Putative Competence Regulon In G Anatismentioning

confidence: 99%

Natural Transformation of Gallibacterium anatis

Kristensen

Sinha

Boyce

et al. 2012

Appl Environ Microbiol

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“…4) (73, 116). G. anatis represents an outgroup of the two main subclades (117,118), suggesting that this species would be an ideal system for investigation of the origins of uptake bias and uptake sequence accumulation. In contrast, Haemophilus ducreyi (Apl-USS subclade) has a substantially lower density of USS than other Pasteurellaceae, suggesting either that it has lost competence or that its uptake specificity is changing (Fig.…”

Section: Figmentioning

confidence: 99%

Natural Competence and the Evolution of DNA Uptake Specificity

2014

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Many bacteria are naturally competent, able to actively transport environmental DNA fragments across their cell envelope and into their cytoplasm. Because incoming DNA fragments can recombine with and replace homologous segments of the chromosome, competence provides cells with a potent mechanism of horizontal gene transfer as well as access to the nutrients in extracellular DNA. This review starts with an introductory overview of competence and continues with a detailed consideration of the DNA uptake specificity of competent proteobacteria in the Pasteurellaceae and Neisseriaceae. Species in these distantly related families exhibit strong preferences for genomic DNA from close relatives, a self-specificity arising from the combined effects of biases in the uptake machinery and genomic overrepresentation of the sequences this machinery prefers. Other competent species tested lack obvious uptake bias or uptake sequences, suggesting that strong convergent evolutionary forces have acted on these two families. Recent results show that uptake sequences have multiple "dialects," with clades within each family preferring distinct sequence variants and having corresponding variants enriched in their genomes. Although the genomic consensus uptake sequences are 12 and 29 to 34 bp, uptake assays have found that only central cores of 3 to 4 bp, conserved across dialects, are crucial for uptake. The other bases, which differ between dialects, make weaker individual contributions but have important cooperative interactions. Together, these results make predictions about the mechanism of DNA uptake across the outer membrane, supporting a model for the evolutionary accumulation and stability of uptake sequences and suggesting that uptake biases may be more widespread than currently thought. N aturally competent bacteria actively pull DNA fragments from their environment into their cells. These fragments provide nucleotides, but high similarity with the chromosome also allows them to change the cell's genotype by homologous recombination, a process called natural transformation ( Fig. 1; reviewed in references 1, 2, 3, 4, and 5). Most competent bacteria that have been tested can take up DNA from any source, but species in two distantly related families of Gram-negative bacteria, the Pasteurellaceae and the Neisseriaceae, show strong preferences for DNAs containing short sequences that are highly overrepresented in their own genomes, leading to preferential uptake of conspecific DNA (6). This self-specificity both raises questions about and provides a tool for investigating the evolution of competence and the mechanism of DNA uptake.We begin with a general overview of competence, emphasizing the evolutionary issues. We then describe the two components that together create self-specificity, sequence biases in the DNA uptake machinery and overrepresentation of uptake sequences in the genomes, highlighting recent work on the mechanism and evolution of uptake biases in the two families and an evolutionary model that accounts for upt...

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“…Comparative genomic and phylogenetic analyses of the Pasteurellaceae have revealed that many members of this highly diverse family were poorly classified (54, 56). Indeed, a number of the Pasteurellaceae have already been renamed: Histophilus somni (formerly Haemophilus somnus, H. agni, and H. ovis) (57), Mannheimia (formerly Pasteurella) haemolytica (58), Bibersteinia (formerly Pasteurella) trehalosi (59), Actinobacillus (formerly Haemophilus) pleuropneumoniae (60), Actinobacillus (formerly Pasteurella) ureae (61), Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans (62), Aggregatibacter aphrophilus (formerly Haemophilus aphrophilus and H. paraphrophilus) (62), Aggregatibacter (formerly Haemophilus) segnis (62), Avibacterium (formerly Haemophilus) paragallinarum (63), Avibacterium (formerly Pasteurella) gallinarum (63), Avibacterium (formerly Pasteurella) volantium (63), Avibacterium (formerly Pasteurella) avium (63), Basfia (formerly Mannheimia) succiniciproducens (64), and Gallibacterium (formerly Pasteurella) anatis (65). However, as can been seen from the 16S rRNA phylogenetic tree shown in Fig.…”

Section: Pasteurella and The Pasteurellaceae Family Comparative Genommentioning

confidence: 99%

Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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SUMMARY In a world where most emerging and reemerging infectious diseases are zoonotic in nature and our contacts with both domestic and wild animals abound, there is growing awareness of the potential for human acquisition of animal diseases. Like other Pasteurellaceae , Pasteurella species are highly prevalent among animal populations, where they are often found as part of the normal microbiota of the oral, nasopharyngeal, and upper respiratory tracts. Many Pasteurella species are opportunistic pathogens that can cause endemic disease and are associated increasingly with epizootic outbreaks. Zoonotic transmission to humans usually occurs through animal bites or contact with nasal secretions, with P. multocida being the most prevalent isolate observed in human infections. Here we review recent comparative genomics and molecular pathogenesis studies that have advanced our understanding of the multiple virulence mechanisms employed by Pasteurella species to establish acute and chronic infections. We also summarize efforts being explored to enhance our ability to rapidly and accurately identify and distinguish among clinical isolates and to control pasteurellosis by improved development of new vaccines and treatment regimens.

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Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen

Cited by 93 publications

References 31 publications

Natural Transformation of Gallibacterium anatis

Natural Transformation of Gallibacterium anatis

Natural Competence and the Evolution of DNA Uptake Specificity

Pasteurella multocida: from Zoonosis to Cellular Microbiology

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