Summary -It is well known that digestibility decreases during growth in alfalfa (Medicago sativa L), but the effects of genetic variation and the environment on digestibility are less documented. Seven cultivars were studied in five locations in France, and four samplings were taken over two regrowth cycles. Forage yield, stem height, number of stems, plant digestibility, stem digestibility and leaf/stem ratio were recorded. All traits were influenced by location, cycle, cultivar and sampling date effects. Digestibility decreased with yield, as plants grew, and leaf/stem ratio also decreased. The regression between plant digestibility and yield was the same for all the cultivars. However, this regression was quite different depending on the location. Digestibility decreased faster in some locations than in others. Location effects were not related to average temperatures. The seven cultivars showed differences in digestibility, as a result of various combinations of leaf/stem ratio and stem digestibility. At