Basic Biology of Pig Fetal Pancreas and Its Use as an Allograft

Tuch, Bernard E.; Simpson, Ann M.; Smith, Murray; Waugh, P.; Weinhaus, Anthony J.; Tu, Jian; Rose, Michael

doi:10.1007/978-1-4615-1981-2_5

Cited by 11 publications

(10 citation statements)

References 20 publications

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“…Juvenile Large White Landrace pigs were anesthetized, and a catheter inserted into the jugular vein (12,13). Juvenile Large White Landrace pigs were anesthetized, and a catheter inserted into the jugular vein (12,13).…”

Section: Pig Studiesmentioning

confidence: 99%

Secretion of Pancreatic Icosapeptide from Porcine Pancreas

et al. 2001

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The pancreatic polypeptide cell, the only mature endocrine cell in the fetal pig pancreas, produces equimolar amounts of two peptides, pancreatic polypeptide and pancreatic icosapeptide, from the same precursor. The amino acid sequence of pancreatic polypeptide is more homogeneous among species, whereas pancreatic icosapeptide is heterogeneous. We determined the 19-amino acid sequence of porcine pancreatic icosapeptide, which is markedly different from that of known sequences (e.g. 47% homology with human). We developed an ELISA that can measure porcine pancreatic icosapeptide levels in the range of 7.2-480 pmol/liter. Actual levels of pancreatic icosapeptide in pig sera were 9.6-25 pmol/liter. The assay requires relatively small amounts of nonextracted samples, and human and mouse sera do not cross-react. Levels of pancreatic icosapeptide rose in response to hypoglycemia in pigs and to carbachol in fetal porcine pancreatic cells in vitro. When fetal porcine pancreatic tissue was transplanted into nonobese diabetic-severe combined immune deficiency mice, porcine pancreatic icosapeptide (but not C peptide) was detectable in mouse sera for up to 3 wk after transplantation, with levels highest on d 4. Porcine pancreatic icosapeptide and insulin were detectable in grafts removed from the mice. Therefore, porcine pancreatic icosapeptide may be used as a marker of the viability of xenotransplanted fetal pig pancreatic tissue in the immediate posttransplant period.

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Section: Pig Studiesmentioning

confidence: 99%

Secretion of Pancreatic Icosapeptide from Porcine Pancreas

et al. 2001

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“…Several studies in similar models have shown that ICCs have a poor insulin response to glucose [18][19][20][21][22][23][24][25][26]. The main problem with ICCs is the need for maturation (3 months) to achieve in-vivo functionality.…”

Section: Tools and Sources For Translation Into Clinical Practicementioning

confidence: 99%

Pig islets for clinical islet xenotransplantation

Dufrane

Gianello²

2009

Current Opinion in Nephrology and Hypertension

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“…They were transported on ice and arrived in the laboratory for processing within 4 h of removal from the pregnant sow. Previous studies conducted in our laboratory have shown that the fetal pancreatic tissue is viable after this period of cold ischaemia [Tuch et al, 1995]. Following removal, the pancreas was washed and dissected into cubes of approximately 1 mm 3 .…”

Section: Preparation and Culture Of Pancreatic Tissue (Iccs)mentioning

confidence: 99%

“…During the culture period of the ICCs, the tissue type used in this study, the cellular aggregates round up and achieve diameters of between 67 and 113 Ìm, with an insulin content of 5.4 B 1.2 mU/20 ICCs [Tuch et al, 1995]. The percentage of insulin producing (ß) cells observed in the ICCs was 5-6% with lower percentages of glucagon, somatostatin and pancreatic polypeptide containing cells being found [Korsgren et al, 1991;Tuch et al, 1995]. The nature of the remaining cells, most of which have been classified as epithelioid using routine histological identification, has yet to be determined [Korsgren et al, 1988[Korsgren et al, , 1991.…”

Section: Introductionmentioning

confidence: 99%

“…The mid to late gestational fetal pancreatic tissue is commonly transplanted as explants or islet-like cell clusters (ICCs) which are produced by partial collagenase digestion followed by a 4-to 5-day culture period [Korsgren et al, 1988;Tuch et al, 1995]. During the culture period of the ICCs, the tissue type used in this study, the cellular aggregates round up and achieve diameters of between 67 and 113 Ìm, with an insulin content of 5.4 B 1.2 mU/20 ICCs [Tuch et al, 1995].…”

Section: Introductionmentioning

confidence: 99%

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In vitro Dedifferentiation of Fetal Porcine Pancreatic Tissue prior to Transplantation as Islet-Like Cell Clusters

Humphrey

Smith²,

Kwok³

et al. 2001

Cells Tissues Organs

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The fetal porcine pancreas under experimental conditions can be transplanted in the form of explants or islet-like cell clusters (ICCs) to normalize blood glucose levels in diabetic recipients. ICCs are released from the collagenase-digested pancreas and require a 4- to 5-day culture period for their complete formation. In order to maximize insulin producing β cell differentiation following transplantation, an understanding of ICC development is essential to utilize this alternative treatment for type 1 diabetes. In this study a role is proposed for exocrine cells in the generation of the multipotent pancreatic precursor cells during the culture period. Acinar cells undergo dedifferentiation during the initial stages of the cultureperiod into multipotent pancreatic precusor cells, previously called protodifferentiated cells. The progressive loss of exocrine differentiation appears to involve rapid degranulation of zymogen granules by exocytosis and loss of the prominent secretory apparatus. These processes occur in parallel with a significant reduction in the expression of lipase in the period from day 0 to day 5 and simultaneously there is an increase in the epithelioid/ductal cell marker, cytokeratin 20. Using proliferating cell nuclear antigen, cell proliferation during the culture period does not appear to account for the increase in epithelioid/ductal cells. Further the rates of apoptosis and necrosis which were identified using the TUNEL technique and propidium iodide, respectively, do not appear to account for the reduction in exocrine cell numbers. Exocrine cell dedifferentiation appears to increase the pool of protodifferentiated cells which have the potential to develop into the insulin-producing β-cell population following transplantation into the diabetic recipient

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Basic Biology of Pig Fetal Pancreas and Its Use as an Allograft

Cited by 11 publications

References 20 publications

Secretion of Pancreatic Icosapeptide from Porcine Pancreas

Secretion of Pancreatic Icosapeptide from Porcine Pancreas

Pig islets for clinical islet xenotransplantation

In vitro Dedifferentiation of Fetal Porcine Pancreatic Tissue prior to Transplantation as Islet-Like Cell Clusters

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