Basic hydrolysis of in the preparation of 2-[18F]fluoro-2-deoxy-d-glucose

Füchtner, F.; Steinbach, Jörg; Mäding, P.; Johannsen, B.

doi:10.1016/0969-8043(95)00258-8

Cited by 75 publications

(28 citation statements)

References 7 publications

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“…The synthesis of [ 18 F]FDG was performed as described previously (Hamacher et al 1986;Fuchtner et al 1996) using an F-120 automatic system (Sumitomo Heavy Industries, Tokyo, Japan). BrieXy, [ 18 F]Xuoride was produced by the nuclear reaction 18 O(p,n) 18 F with an HM-18 cyclotron (Sumitomo Heavy Industries).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Incorporation and translocation of 2-deoxy-2-[18F]fluoro-d-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system

Hattori

Uchida

Harada

et al. 2008

Planta

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[(18)F]FDG (2-deoxy-2-[(18)F]fluoro-D-glucose) was fed to a sorghum plant [Sorghum bicolor (L.) Moench] from the tip of a leaf and its movement was monitored using a planar positron imaging system (PPIS). [(18)F]FDG was uptaken from the leaf tip and it was translocated to the basal part of the shoots from where it moved to the roots, the tillers and the sheaths. Autoradiographic analysis of the distribution of (18)F, [(18)F]FDG and/or its metabolites showed translocation to the roots, tillers, and to the leaves that were younger than the supplied leaf. Strong labelling was observed in the basal part of the shoots, in the sheaths, the youngest leaf and the root tips. Our results indicate that [(18)F]FDG and/or its metabolites were absorbed from the leaf and translocated to the sites where nutrients are required. This strongly suggests that [(18)F]FDG can be utilised as a tracer to study photoassimilate translocation in the living plant. This is the first report on the use of [(18)F]FDG, which is routinely used as a probe for clinical diagnosis, to study source to sink translocation of metabolites in whole plants in real time.

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Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Incorporation and translocation of 2-deoxy-2-[18F]fluoro-d-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system

Hattori

Uchida

Harada

et al. 2008

Planta

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“…However, detailed investigations of the toxicity of C1DG and a comparison of its possible toxicity with that of FDG have not yet been carried out. By using other mineral acids or sodium hydroxide for the hydrolysis of the precursors formation of C1DG can be avoided [6]. [7].…”

Section: -Chloro-2-deoxy-d-glucose (Cldg)mentioning

confidence: 99%

PET radiopharmaceuticals in Europe: Current use and data relevant for the formulation of summaries of product characteristics (SPCs)

et al. 1995

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“…Briefly, 18 F was produced by the nuclear reaction of 18 O(p,n) 18 F using a cyclotron system (Cypris HM-18; Sumitomo Heavy Industries, Ltd.). According to the method of Füchtner et al (11) with a minor modification, 18 F-FDG was automatically synthesized within 40 min using a radiochemical system (F-200; Sumitomo Heavy Industries, Ltd.).…”

Section: F-fdg Preparationmentioning

confidence: 99%

Establishment of In Vivo Brain Imaging Method in Conscious Mice

Mizuma¹,

Shukuri²,

Hayashi³

et al. 2010

J Nucl Med

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In vivo imaging, such as PET, requires restriction of body movements and is generally conducted under sedation by anesthetic agents in studies using laboratory animals. Because anesthetics reduce neural activity and metabolism, physiologic neural functions are difficult to assess in animal PET studies. Therefore, use of an appropriate method in conscious animals is important and is a practical requirement for physiologic in vivo brain imaging studies. Here, we established an in vivo imaging system for conscious mice to reveal the physiologic regional cerebral glucose metabolic rate (rCMRglu) with 18 F-FDG PET. Methods: We first developed a head holder to enable brain PET of a conscious mouse. To obtain optimal rCMRglu, we examined the effects of physical and psychologic stresses caused by ambient temperature, intravenous injection, and acclimation to the apparatus and immobile state. Finally, quantitative kinetic analysis was performed for rCMRglu based on a 2-tissue-compartment model with an input function of arterial blood sampling under both conscious and anesthetized (1.5% isoflurane) conditions. Results: Increasing the ambient temperature increased uptake of 18 F-FDG in the brain significantly while reducing the uptake in skeletal muscle and brown adipose tissue that was caused by shivering. The reduction of brain 18 F-FDG uptake caused by tail holding and manual injection was significantly ameliorated by the use of an automated slow injection. Although brain uptake of 18 F-FDG varied at the first session of PET, uptake at the second and subsequent sessions was stable, even after long-term acclimation. After these beneficial changes, brain uptake of 18 F-FDG improved significantly, to approximately 260% above the preconditioned state, which is comparable with that obtained in mice that have been allowed to move freely about their home cages. Quantitative kinetic analyses revealed that isoflurane anesthesia lowered rCMRglu in the cerebral cortex, striatum, thalamus, and cerebellum by 66%, 59%, 62%, and 22%, respectively, mainly by reducing the k 3 value, a rate constant for phosphorylation by hexokinase. Conclusion: To our knowledge, this is the first study to report quantitative kinetic analysis of rCMRglu in mice that have been conscious throughout PET. This investigation will open avenues for research into in vivo functional brain molecular imaging in both normal and genetically manipulated mice.

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Basic hydrolysis of in the preparation of 2-[18F]fluoro-2-deoxy-d-glucose

Cited by 75 publications

References 7 publications

Incorporation and translocation of 2-deoxy-2-[18F]fluoro-d-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system

Incorporation and translocation of 2-deoxy-2-[18F]fluoro-d-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system

PET radiopharmaceuticals in Europe: Current use and data relevant for the formulation of summaries of product characteristics (SPCs)

Establishment of In Vivo Brain Imaging Method in Conscious Mice

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