Basic Peroxidases and Rooting in Microcuttings of Malus

Abstract

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Ethylene and rooting of mung bean cuttings. The role of auxin induced ethylene synthesis and phase-dependent effects

“…However, the ethylene level should be measured in the tissue from where the roots regenerate, viz., in the part of the stem that is immersed in the medium. In this portion of the cutting, the ethylene level is expectedly much higher because the auxin concentration in the lower part of the shoot is higher (e.g., De Klerk et al 1990) and because ethylene cannot easily escape from immersed tissues (cf. Voesenek et al 1993).…”

Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots

“…Although the increase in peroxidase activity preceding rooting has generally been noticed, some research workers were unable to demonstrate the peak and subsequent decrease in enzyme activity (Gebhardt, 1985;Pythoud and Buchala, 1986;Patience and Alderson, 1987;de Klerk et al, 1990). The explanation of these discrepancies may stem from the fact that in these studies, peroxidase activity was measured in purified extracts and/or not expressed per unit protein.…”

“…We have further shown (Aghmir et al, 1991) (Nakano et al, 1980;Fouret et al, 1986;Welander and Snygg, 1987;Gaspar and Hofinger, 1988 . A positive correlation also occurred between the activity of (basic) peroxidases in stem of shoots of apple at the time of transfer to rooting medium and the number of roots formed later (Gus'kov et al, 1988;de Klerk et al, 1990 Benzyladenine used instead of kinetin in the multiplication medium of Asparagus provided a shoot population with a higher peroxidase activity and a higher rooting percentage . Continuous darkness instead of a 16-8 day-night cycle during elongation and root initiative phases of apple shoots favoured successive increase and decrease of peroxidase activity, and resulted in higher percentages of rooted plantlets (Druart et al, 1982).…”

Effect of accidental factors on rooting of Malus Golden Delicious

“…The conditions during propagation and rooting were as described by De Klerk et al (1990). Shoots 1-2 cm in length were used for rooting.…”