Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots

Gaspar, Th.; Kevers, Claire; Hausman, Jean-François; Berthon, Jean‐Yves; Ripetti, V.

doi:10.1051/agro:19921003

Cited by 105 publications

(68 citation statements)

References 18 publications

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“…According to Gaspar et al (1992), it is necessary to detect maximum and minimum peroxidase activity to define the end or the beginning of the rooting phases. In this study, significant differences were not detected in the total peroxidase activity peaks, to allow the definition of the rooting phases.…”

Section: Determination Of Rooting Phasesmentioning

confidence: 99%

Peroxidase and polyamine activity variation during thein vitrorooting ofBerberis buxifolia

Arena

Pastur²,

Benavides

et al. 2003

New Zealand Journal of Botany

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Berberis buxifolia is a Patagonian shrub with great economic potential for tinctorial, pharmacological, and food industries. Clonal propagation is possible through in vitro culture and is also useful for metabolite production. However, this species is difficult to root, and to improve this, more knowledge of rhizogenesis processes is needed. Polyamines and peroxidases are useful biochemical markers during analysis of rooting phases for correlation with tissue morphological changes. Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes, peroxidase activity evolution, and morphological development were studied to characterise the in vitro rhizogenesis of microshoots of B. buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase changed significantly during the rooting period, and had opposite behaviours which were directly related to the IBA media content. The lower polyamine concentration and the higher peroxidase activity were found in a treatment with a dark period during the first four days and with IBA in the culture medium. Putrescine was the most abundant polyamine found in B. buxifolia tissues, 14-to 18-fold more than spermidine and spermine, respectively. Therefore, these compounds were used to define the rooting phases: an induction phase (0 to 4-7 days) followed by an expression phase (4-7 to 28 days). The observed changes in the biochemical markers could be correlated with microscopic and macroscopic tissue observations in the microshoots, and the time course of rooting percentage. Successive culture media can be developed including polyamines, or other compounds and environmental conditions, which positively modify the studied biochemical markers behaviour.

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Section: Determination Of Rooting Phasesmentioning

confidence: 99%

Peroxidase and polyamine activity variation during thein vitrorooting ofBerberis buxifolia

Arena

Pastur²,

Benavides

et al. 2003

New Zealand Journal of Botany

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“…POD is a highly active enzyme, can participate in a variety of physiological processes in the plant and the formation of lignin, and adventitious root induction and elongation growth is closely related to oxidize IAA, to eliminate the body too much endogenous IAA, with some higher plants The development process is closely related. The activity of POD is closely related to the rooting of in vitro plants, which is one of the landmarks of plant rooting [7]. In the adventitious root induction and expression period, the increase of POD activity is a sign of rooting ability [8].…”

Section: Results and Analysismentioning

confidence: 99%

Study on the Mechanism of the Regulation of NAA Promoted the Adventitious Root Formation of the Dalbergia Odorifera.T.Chen Hardwood Cuttings

Wang¹,

Li²,

Zhang³

et al. 2017

Proceedings of the 2017 5th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering (ICMMCCE 20

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Abstract. In order to reveal the rooting mechanism of Dalbergia odorifera.T.Chen, the rooting mechanism of Dendrocalamopsis mongolica was 4,600 mg / L NAA rooting (POD), polyphenol oxidase (PPO), superoxide dismutase (SOD), indole (SOD), indoxylate (SOD), indoxidase Acetic acid oxidase (IAAO) and other root-related enzyme activity changes. The results showed that the rooting type of callus was decreased by 600 mg / L NAA, and the rooting rate and rooting quality were obviously improved. The activity of PPO, SOD and IAAO increased gradually after cutting, reached the peak at the time of root formation, and then decreased gradually. The activity of PPO, SOD and IAAO increased gradually after cutting. The activity of POD enzyme increased gradually after cutting, reached the peak at the root formation stage, then gradually decreased, then gradually increased, reached another peak at the root elongation, and finally decreased gradually, showing a bimodal trend.

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“…One of the main achievements in the study of adventitious rooting has been the recognition of interdependent phases (induction, initiation and expression) (Gaspar et al, 1992). These phases have been identified through variation of some biochemical markers.…”

Section: Introductionmentioning

confidence: 99%

“…These phases have been identified through variation of some biochemical markers. At present, peroxidase is asserted to be the best rooting phase marker for rooting of cuttings (Gaspar et al, 1992;Arena et al, 2003;Metaxas et al, 2004;Syros et al, 2004;Naija et al, 2008;). The induction phase of rooting, before any visible morphological and histological events, is characterized by a decline in peroxidase activity, and the minimum of peroxidase activity indicates termination of this phase.…”

Section: Introductionmentioning

confidence: 99%

Can Changes in Starch Content and Peroxidase Activity Be Used as Rooting Phase Markers for Rhododendron Leaf Bud Cuttings?

Megre¹,

Dokane²,

Kondratovics³

2011

Acta Biologica Cracoviensia Series Botanica

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We examined whether peroxidase activity in cutting bases and leaves and starch content in cutting bases can be used as rooting phase markers in the elepidote rhododendron cv. 'Babites Baltais' (Rhododendron L.). Changes in peroxidase activity in cutting leaves and bases, as well as starch content in cutting bases, were determined in relation to anatomical stages of rhizogenesis in leaf bud cuttings treated with 1% indole-3-butyric acid (IBA+) or without IBA (IBA-). The pattern of change of peroxidase activity was similar in cutting bases and leaves of IBAleaf bud cuttings. Three phases of adventitious root formation were identified: induction, initiation and expression. During the induction phase peroxidase activity decreased, but no anatomical changes were observed in the cuttings. Peroxidase activity increased in the initiation phase when adventitious root initials were formed. Peroxidase activity decreased during the expression phase when adventitious root primordia developed. The starch content of IBA-leaf bud cuttings decreased during the first few days and then gradually rose to maximum, followed by a sharp reduction and another increase at the end of the experiment. The changes of starch content did not coincide with rooting phases as peroxidase activity did, and cannot be used as a rooting phase marker in rhododendrons. Adventitious root formation did not occur in IBA+ leaf bud cuttings, so distinct rooting phases could not be observed. There was a significant correlation between peroxidase activity in cutting bases and leaves of IBA-leaf bud cuttings. Peroxidase activity in leaves of rhododendron leaf bud cuttings are potentially useful as a marker for rooting phases, but that requires further anatomical and physiological study of rooting in leaf bud cuttings.K Ke ey y w wo or rd ds s: : Peroxidase, starch, adventitious root, leaf bud cuttings, rhododendron.

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Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots

Abstract: Summary — A peak of specific peroxidase activity (increase in enzyme activity followed by decrease) has generally been found in crude extracts of stem-or shoot-cuttings prior to root formation. Effectors

Cited by 105 publications

References 18 publications

Peroxidase and polyamine activity variation during thein vitrorooting ofBerberis buxifolia

Peroxidase and polyamine activity variation during thein vitrorooting ofBerberis buxifolia

Study on the Mechanism of the Regulation of NAA Promoted the Adventitious Root Formation of the Dalbergia Odorifera.T.Chen Hardwood Cuttings

Can Changes in Starch Content and Peroxidase Activity Be Used as Rooting Phase Markers for Rhododendron Leaf Bud Cuttings?

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