Basic procedure for the in vitro propagation of Brazilian trees for reforestation purposes

Abstract

A R T I C L E I N F O Received 06 Feb 2017 Accepted 18 Apr 2017 Published 02 Mai 2017A B S T R A C T Important strategies can be used to avoid biodiversity loss by deforestation in tropical rainforests. Some use biotechnological techniques to support conservation initiatives. Plant tissue culture techniques are highly accepted biotechnological approaches for conservation of biodiversity. The work aimed to propose a basic operational model for the induction of in vitro germination of trees through plant tissue cultivation techniques. Fruits of 15 tree species, ten woody trees (Couroupita guianensis Aubl., Tabebuia heptaphylla (Vell.) Toledo, Tabebuia impetiginosa (Mart. ex DC.) Standl., Tabebuia roseoalba (Ridl.) Sandwith, Vochysia haenkeana (Spreng.) Mart., Vitex montevidensis Cham., Copaifera coriacea Mart., Spondias tuberosa Arruda, Schinus terebinthifolia Raddi, and Talisia esculenta (A. St.-Hil.) Radlk.) and five palm trees (Syagrus coronate (Mart.) Becc., Attalea oleifera Barb. Rodr., Elaeis guineensis Jacq., Colubrina glandulosa Perk., and Astrocaryum vulgare Mart.) were collected at different locations in the State of Pernambuco, Brazil. The in vitro germination used two different protocols, one designed for palm trees and one designed for woody trees. It was evaluated the parameters microbial contamination, survival, in vitro establishment, germination percentage and percentage of seeds converted to plants. The results showed that the set of methodologies proposed as a basic protocol for the in vitro introduction was able to achieve satisfactory results for 13 of the 15 tested species. The protocol proposed a high potential for use in the rescue of seeds through in vitro plant tissue culture. The described technique is an efficient tool for the propagation of trees used in reforestation programs.

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

“…Our results suggest that the protocol used in this work was adequate for this species and can be used routinely, being a valid procedure for the propagation of other Pinus species as well. The most important initial step in the micropropagation process is an adequate sterilization protocol that minimizes tissue damage to achieve the greatest number of aseptic explants ready to be used in the next phases of micropropagation [ 27 , 29 ].…”

Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

“…Nowadays, redwood shows seed reproduction difficulties, displaying low germination and rooting rates, dormancy of the shoot, and low seedling viability 5,6 . Therefore, biotechnological in vitro techniques of plant tissue culture as micropropagation, organogenesis, adventitious or axillary shoot/bud regeneration, shoot tip culture, micrografting, and somatic embryogenesis emerged as useful tools for the propagation and conservation of germoplasm 7,8,9 .…”

Can our current knowledge and practice allow ecological restoration in the Cerrado?

“…We suggest future studies should map and compute these aspects, fostering a more precise discussion. Besides, the low attention given to some vegetation types, such as campo limpo, campo sujo, campo cerrado, quartzitic rupestrian grassland and ironstone outcrops, could be related to limited or scarce knowledge on natural regeneration (Silveira et al 2016, but see Luz et al 2018) and the lack of knowledge about requirements of native species cultivation (De Souza et al 2017, Fernandes et al 2016c. The difficulties in recognizing and determining each vegetation type, and the fact that restoration has been historically attached to forest ecosystems and tree usage, also hamper attempts to ecologically restore the natural heterogeneity of the Cerrado (see Fernandes et al 2020).…”